Báo cáo khoa học: " Characterization of the Recombinant Proteins of Porcine ircovirus Type 2 Field Isolate Expressed in the Baculovirus System" potx
Báo cáo khoa học: " Characterization of the Recombinant Proteins of Porcine ircovirus Type 2 Field Isolate Expressed in the Baculovirus System" potx
... (Fig. 2) . Characterization of the Recombinant Proteins of Porcine Circovirus Type2 Field Isolate Expressed in the Baculovirus System 21 Fig. 1. Cloning of the ORF 1 and 2 of the PCV type2 in pVL ... conditions for the amplification was Characterization of the Recombinant Proteins of Porcine Circovirus Type2 Field Isolate Expres...
Ngày tải lên: 07/08/2014, 15:20
Báo cáo khoa học: NMR and MS evidences for a random assembled O-specific chain structure in the LPS of the bacterium Xanthomonas campestris pv. Vitians A case of unsystematic biosynthetic polymerization potx
... Mangoni on the occasion of his 70th birthday. (Received 1 March 20 02, revised 26 June 20 02, accepted 25 July 20 02) Eur. J. Biochem. 26 9, 4185–4193 (20 02) Ó FEBS 20 02 doi:10.1046/j.14 32- 1033 .20 02. 03138.x Therefore, ... showing the lacking of fragment ions corresponding to the presence of a reducing end Rha or a nonreducing end Rha. 41 92 A. Molinaro et al.(Eur...
Ngày tải lên: 31/03/2014, 09:20
Báo cáo khoa học: Etoposide upregulates Bax-enhancing tumour necrosis factor-related apoptosis inducing ligand-mediated apoptosis in the human hepatocellular carcinoma cell line QGY-7703 pdf
... function of TRAIL containing the extracellular domain (amino acids 114 28 1) of the human liver cancer cell line, QGY-7703 [27 ], we treated the cells with increasing concentrations of TRAIL and then analysed ... procaspase-9 and caspase-3, by binding to the inhibitor of apoptosis proteins and thus disrupts linkage of the caspase–inhibitor of apoptosis proteins compl...
Ngày tải lên: 23/03/2014, 17:22
Báo cáo khoa học: Ca2+-binding allergens from olive pollen exhibit biochemical and immunological activity when expressed in stable transgenic Arabidopsis pdf
... & Rodriguez R (20 02) Are Ca 2+ -binding motifs involved in the immunoglobulin E-binding of allergens? Olive pollen allergens as model of study. Clin Exp Allergy 32, 1476–1483. 25 Tacket CO, Mason ... in- gel digestion of the protein with trypsin. The resulting peptides were analysed by MALDI-TOF. The molecular mass of one of these peptides fits well with the N-t...
Ngày tải lên: 30/03/2014, 10:20
Báo cáo khóa học: Volkensin from Adenia volkensii Harms (kilyambiti plant), a type 2 ribosome-inactivating protein pptx
... carbohydrate binding site 2 of the volkensin B-chain is weakly functional, further studies on binding affinity and docking of galactose and GalNAc in both binding sites 1 and 2 of the volkensin B-chain are required. ... [20 ]. Interestingly, in spite of the high degree of conservation of amino acid residues involved both in the A-chain active sites and in the...
Ngày tải lên: 30/03/2014, 13:20
Báo cáo khoa học: Characterization of the tRNA and ribosome-dependent pppGpp-synthesis by recombinant stringent factor from Escherichia coli pot
... set of recombinant small subunit ribo- somal proteins. RNA 5, 8 32 843. 26 Nirenberg M & Leder P (1964) RNA code words and protein synthesis. The effect of trinucleotides upon the binding of ... 27 2 (20 05) 685–695 ª 20 05 FEBS 685 A-site footprint at G530 in 16S rRNA (Fig. 6D, red line [28 ]) and A-site footprints in domain IV in 23 S rRNA at nucleotides C1941 and...
Ngày tải lên: 07/03/2014, 16:20
Báo cáo khoa học: Characterization of recombinant forms of the yeast Gas1 protein and identification of residues essential for glucanosyltransferase activity and folding pot
... of products from the incubation of the recombinant sGas1 523 p, sGas1E161Q-H and sGas1E262Q- H mutant proteins with reduced laminari- oligosaccharides. The recombinant purified proteins were incubated ... multidomain proteins [28 ], or for the sequential and independent fold ing of the single domains – assuming a vectorial mode of folding for Gas1p. In any case,...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo khoa học: Characterization of native and recombinant A4 glyceraldehyde 3-phosphate dehydrogenase Kinetic evidence for conformation changes upon association with the small protein CP12 pptx
... BPGA concentration. All the experimental points were fitted to a sigmoid (Eqn 2 in the main text). Detailed fitting of the first points is given in the inset. (B) Recombinant (3 · 10 )9 M , d) and ... volume of 1.5 mL at 30 °C for 20 min. The concentration of BPGA inthepresenceof0 .25 m M NADH was determined using excess rabbit muscle GAPDH and 10 lL of the previous...
Ngày tải lên: 23/03/2014, 20:22
Báo cáo khoa học: Characterization of recombinant prolidase from Lactococcus lactis – changes in substrate specificity by metal cations, and allosteric behavior of the peptidase pdf
... and T. Tanaka 27 4 FEBS Journal 27 5 (20 08) 27 1 28 0 ª 20 07 The Authors Journal compilation ª 20 07 FEBS the catalytic properties of porcine pepsin A. Protein Pept Lett 5, 19 26 . 22 Kato H, Tanaka ... little information on the expression of recombinant prolidases, the functionality of each residue or protein engineering of this enzyme. In the present study,...
Ngày tải lên: 30/03/2014, 04:20
Tài liệu Báo cáo khoa học: Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas docx
... sequences of the catalytic domain of members of the GH18 family resulted in the amplification of an expected 147 bp sequence. Cloning and sequencing of this fragment revealed an ORF showing amino acid ... pattern of expres- sion of the gene encoding Cg-Clp2 during oyster devel- opment were established by real-time PCR and in situ hybridization. In addition, the in...
Ngày tải lên: 19/02/2014, 00:20