... due to a distribution of partially unfolded conformations. At a protein concentration of 5 lm, the monomeric species appears to predominate in the case of Y74W*. The gel filtration results indicate that ... con- centration range 0.625–40 lm, but the Y74W* mutant shows a sharp dependence of emission wavelength on protein concentration. At the lowest concentration examined, 0.625 lm, t...
Ngày tải lên: 18/02/2014, 11:20
... the membrane of cells. Inhibitory effects of VVA1 on the hemolytic and cytotoxic activity of VVA2 The effects of VVA1 on the hemolytic activity of VVA2 were studied by incubation of human red ... Rochat H, Harvey AL, Montenay-Garestier T & Menez A (1990) Delineation of the functional site of a snake venom cardiotoxin: preparation, structure, and function of monoa...
Ngày tải lên: 19/02/2014, 06:20
Báo cáo khoa học: Mutual effects of proton and sodium chloride on oxygenation of liganded human hemoglobin Oxygen affinities of the a and b subunits potx
... conditions were used as follows: 8.5, 7.4, and 6.8. The NaCl effects were carried out at concentrations of 0.1, 0.5, 1.0, and 2.0 m. The solubility of O 2 in water depends strongly on NaCl concentration. ... approach is based on determination of the bimolecular association rate constant of O 2 rebinding and the quantum yield of BR, c. The latter value is defined as the ratio o...
Ngày tải lên: 16/03/2014, 14:20
Báo cáo khoa học: Membrane distribution of epidermal growth factor receptors in cells expressing different gangliosides doc
... cells from clone 2, and that GD3 and EGFr co-localized, to some extent, only in the absence of EGF stimulation, while, upon addition of EGF, a clear separation of these two membrane components was ... high concentration of ErbB2, which is regulated by lipid rafts, strongly influence homoassociation and the ligand-independent activation of ErbB2 [35]. Considering that the orphan ErbB2...
Ngày tải lên: 16/03/2014, 16:20
Báo cáo khoa học: "Dependency Parsing of Japanese Spoken Monologue Based on Clause Boundaries" docx
... accuracy of our method was higher than that of the conventional method. In this section, we first discuss the effect of our method on parsing accuracy, separately for bun- Table 6: Comparison of parsing ... Section 2.3 and confirmed 70.6% (522/751) of the final bunsetsus of clause boundary units depended on the final bunsetsu of other clause boundary units. 6 Here, the specificat...
Ngày tải lên: 17/03/2014, 04:20
Tài liệu Báo cáo khoa học: Antimicrobial effects of H4-(86–100), histogranin and related compounds – possible involvement of DNA gyrase ppt
... His- tone H4 contains two a-helical regions. One region spans amino acids 55–67 [32]. A second region, that spans amino acids 70–90, has been demonstrated to adopt the a-helical conformation when ... D 650 nm of 0.02 absorbance units were plotted against log(C_0) to generate a calibration curve that related the threshold times to cell concentration at incubation time of zero (C_0). The ra...
Ngày tải lên: 18/02/2014, 14:20
Tài liệu Báo cáo khoa học: Differential effects of Mxi1-SRa and Mxi1-SRb in Myc antagonism ppt
... Evolutionary relationships and functional conservation among verte- brate Max-associated proteins: the zebra fish homolog of Mxi1. Oncogene 9, 3167–3177. C. Dugast-Darzacq et al. Distinct functions of Mxi1 ... as transcription factors that control various aspects of cellular behavior, including cell growth, proliferation, differentiation, apoptosis, genomic stability, and tumorigenesis...
Ngày tải lên: 18/02/2014, 16:20
Tài liệu Báo cáo khoa học: Functional effects of deleting the coiled-coil motif in Escherichia coli elongation factor Ts pptx
... ensure that the concentrations of ligands were maintained at a constant level during migration of the elongation factors, thereby favouring equilibrium conditions. The concentration of guanine nucleotides required ... percentage of the corresponding growth rate of the wild-type strain ( 0.32 h )1 ), was only 55%. Fig. 3. Growth rates. Diagram showing the growth rate of stra...
Ngày tải lên: 21/02/2014, 00:20
Báo cáo khoa học: Differential effects of RU486 reveal distinct mechanisms for glucocorticoid repression of prostaglandin E2 release docx
... expression (Figs 1 and 2 ). In ad dition, the ability of RU486 to antagonize the repressive effects of 0.1 l M dexamethasone or budesonide was examined. In each case, a concentration-dependent antagonism ... repression of COX-2 and COX/PGES activities in these cells. By contrast, the inhibition of NF-jB-dependent tran- scription by high c oncentrations of RU486 correlated v ery cl...
Ngày tải lên: 07/03/2014, 16:20
Báo cáo khoa học: Modulatory effects of plant phenols on human multidrug-resistance proteins 1, 4 and 5 (ABCC1, 4 and 5) potx
... than control pcDNA–HEK293 cells. Nontoxic concentra- tions of each polyphenol were used in combination with increasing concentrations of etoposide to deter- mine the effects of the polyphenols on ... accumulation of the fluorescent substrates in both MRP1- and MRP5-expressing cells in a concentration-dependent manner (data not shown), with concentrations nee- ded to achieve 50% of...
Ngày tải lên: 07/03/2014, 21:20