Báo cáo khoa học: " Angiogenesis effects of nerve growth factor (NGF) on rat corneas" docx

Tài liệu Báo cáo khoa học: Structural effects of a dimer interface mutation on catalytic activity of triosephosphate isomerase The role of conserved residues and complementary mutations pptx

... due to a distribution of partiallyunfolded conformations. At a protein concentration of 5 lm, the monomeric species appears to predominate inthe case of Y74W*. The gel ﬁltration results indicatethat ... con-centration range 0.625–40 lm, but the Y74W* mutantshows a sharp dependence of emission wavelength on protein concentration. At the lowest concentrationexamined, 0.625 lm, the emission maximum ... enhancement of activity of 21.9-foldover the concentration range 2.5–40 lm, strongly sug-gesting that the loss of activity at low concentration maybe attributed to subunit dissociation. In contrast,...

Tài liệu Báo cáo khoa học: Inhibitory effects of nontoxic protein volvatoxin A1 on pore-forming cardiotoxic protein volvatoxin A2 by interaction with amphipathic a-helix doc

... themembrane of cells.Inhibitory effects of VVA1 on the hemolytic andcytotoxic activity of VVA2The effects of VVA1 on the hemolytic activity of VVA2 were studied by incubation of human red ... RochatH, Harvey AL, Montenay-Garestier T & Menez A(1990) Delineation of the functional site of a snakevenom cardiotoxin: preparation, structure, and function of monoacetylated derivatives. ... the inhibition of VVA2 cytotoxic pore formation by VVA1 can onlyoccur at the ideal ratio of 2 : 1 or lower, due to theability of one molecule of VVA1 to interact with twomolecules of VVA2. At...

Báo cáo khoa học: Mutual effects of proton and sodium chloride on oxygenation of liganded human hemoglobin Oxygen afﬁnities of the a and b subunits potx

... conditionswere used as follows: 8.5, 7.4, and 6.8. The NaCl effects were carried out at concentrations of 0.1, 0.5, 1.0, and2.0 m. The solubility of O2in water depends strongly on NaCl concentration. ... approach isbased on determination of the bimolecular associationrate constant of O2rebinding and the quantum yield of BR, c. The latter value is deﬁned as the ratio of thenumber of O2, which ... at four NaCl concentrations of 0.1, 0.5, 1.0 and 2.0 m. The rate constant of BR andthe quantum yield of BR, in the presence of NaCl,gave a direct evidence of signiﬁcant functional hetero-geneity...

Báo cáo khoa học: Membrane distribution of epidermal growth factor receptors in cells expressing different gangliosides doc

... cells fromclone 2, and that GD3 and EGFr co-localized, to someextent, only in the absence of EGF stimulation, while, uponaddition of EGF, a clear separation of these two membranecomponents was ... high concentration of ErbB2,which is regulated by lipid rafts, strongly inﬂuencehomoassociation and the ligand-independent activation of ErbB2 [35]. Considering that the orphan ErbB2 is theonly ... notion of ahomogeneous membrane distribution of the growth factor receptor.Our initial evidence demonstrated the behaviour of different gangliosides expressed in CHO-K1 cells to extrac-tion...

Báo cáo khoa học: "Dependency Parsing of Japanese Spoken Monologue Based on Clause Boundaries" docx

... accuracy of ourmethod was higher than that of the conventionalmethod.In this section, we ﬁrst discuss the effect of ourmethod on parsing accuracy, separately for bun-Table 6: Comparison of parsing ... Section 2.3and conﬁrmed 70.6% (522/751) of the ﬁnal bunsetsus of clause boundary units depended on the ﬁnal bunsetsu of otherclause boundary units.6Here, the speciﬁcations of these annotations ... In Proc. of 6th CoNLL, pages 63–69.S. Kurohashi and M. Nagao. 1994. A syntactic analy-sis method of long Japanese sentences based on thedetection of conjunctive structures. ComputationalLinguistics,...

Tài liệu Báo cáo khoa học: Antimicrobial effects of H4-(86–100), histogranin and related compounds – possible involvement of DNA gyrase ppt

... His-tone H4 contains two a-helical regions. One regionspans amino acids 55–67 [32]. A second region, thatspans amino acids 70–90, has been demonstrated toadopt the a-helical conformation when ... D650nm of 0.02 absorbanceunits were plotted against log(C_0) to generate a calibrationcurve that related the threshold times to cell concentrationat incubation time of zero (C_0). The rate of ... thatmerits our attention. The site of action of quinoloneantibiotics has been determined to be on the gyrA sub-unit of the enzyme, due to the ability of the mutation of Ser83 to Trp to induce...

Tài liệu Báo cáo khoa học: Differential effects of Mxi1-SRa and Mxi1-SRb in Myc antagonism ppt

... Evolutionaryrelationships and functional conservation among verte-brate Max-associated proteins: the zebra ﬁsh homolog of Mxi1. Oncogene 9, 3167–3177.C. Dugast-Darzacq et al. Distinct functions of Mxi1 ... astranscription factors that control various aspects of cellular behavior, including cell growth, proliferation,differentiation, apoptosis, genomic stability, andtumorigenesis [1–4]. Deregulation of ... the onset of foci formation. Assuch, we generated several expression constructs forMxi1-SRa containing different lengths of 5¢-UTR, andtested these in in vitro transcription ⁄ translation assaysfollowed...

Tài liệu Báo cáo khoa học: Functional effects of deleting the coiled-coil motif in Escherichia coli elongation factor Ts pptx

... ensure that the concentrations of ligandswere maintained at a constant level during migration of the elongation factors, thereby favouring equilibriumconditions. The concentration of guanine nucleotidesrequired ... percentage of the corresponding growth rate of the wild-type strain ( 0.32 h)1), was only55%.Fig. 3. Growth rates. Diagram showing the growth rate of strainGRd.tsf as a function of the growth rate ... studied often consist of ÔrestrictedÕ media, in which the concentration of growth substrates is limited, rather than investigating the ability of cells to take up and utilize an unlimited concentration...

Báo cáo khoa học: Differential effects of RU486 reveal distinct mechanisms for glucocorticoid repression of prostaglandin E2 release docx

... expression (Figs 1 and 2 ). In ad dition, the ability of RU486 to antagonize the repressive effects of 0.1 lMdexamethasone or budesonide was examined. In each case,a concentration-dependent antagonism ... repression of COX-2and COX/PGES activities in these cells.By contrast, the inhibition of NF-jB-dependent tran-scription by high c oncentrations of RU486 correlated v eryclosely, in terms of both ... dexamethasone or budesonide. I n fact,RU486 resulted in the progressive repression of PGE2release at increasing concentrations. Analysis of RU486alone on IL-1b-induced PGE2release revealed a concentra-tion-dependent...

Báo cáo khoa học: Modulatory effects of plant phenols on human multidrug-resistance proteins 1, 4 and 5 (ABCC1, 4 and 5) potx

... thancontrol pcDNA–HEK293 cells. Nontoxic concentra-tions of each polyphenol were used in combinationwith increasing concentrations of etoposide to deter-mine the effects of the polyphenols on ... accumulation of the ﬂuorescent substrates in both MRP1- andMRP5-expressing cells in a concentration-dependentmanner (data not shown), with concentrations nee-ded to achieve 50% of the maximum ... polyphenol concentrations. Furthermore,recent studies show a correlation between the in vitro effects of ﬂavonoids in the low micromolar range andin vivo work using oral solutions of ﬂavonoids [13,14].Many...

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