Báo cáo khoa học: "Studies of cocktail therapy with multiple cytokines for neoplasia or infectious disease of the dog" pps

... con- version to other forms. In order to investigate the probable reason(s) for the observed difference, the pro- teins were processed for in-column refolding. The absorption spectra of the in-column ... at 37 °C for 3 h led to the formation of light brown inclu- sion bodies. However, induction at 16 °C for 20 h resulted in the expression of  10–20% of each WhiB...

Ngày tải lên: 18/02/2014, 12:20

... that the K d values for these two receptors were similar; however, the density of the D 1 MUT (404AA405) was Table 1. Binding parameters for the dopamine receptors. For dopa- mine D 2 receptor ... despite the presence of both fluoro- phores in the sample. Figure 3C,D shows the emission profiles of cells cotransfected with plasmids encoding genes for the same type...

Ngày tải lên: 07/03/2014, 03:20

... analysed using the method of Bradford [13]. Syntheses of 4-substituted benzoylformates Syntheses were performed according to Hallmann and Ha ¨ gle [14] and Sultanov [15] by oxidation of the corres- ponding ... 4-substituted benzoylformates with a higher affinity than the unsubsti- tuted benzoylformate as is the case in ScPDC. With the exception of 4-methoxybenzoylformate...

Ngày tải lên: 08/03/2014, 02:20

... required for the cofactor inhibition of phosphorylation (data not shown). The molecular basis for the negative modulatory effects of the pterin cofactor and dopamine binding to wt-hPAH and their ... activation. The inhibited forms of the enzyme with bound biopterin cofactor or dopamine The phosphorylation site in PAH (Ser16) is localized in the N-terminal tail (r...

Ngày tải lên: 17/03/2014, 09:20

... lower than we measured for the isolated NBDs of SUR1. We specu- late that the presence of the transmembrane domains in SUR1 induces conformational changes in the NBDs, or in their association, that ... concentrations of around 0.1 mm or greater [6]. This is in agreement with the results we report here for SUR1 and those found previously for the K ATP complex [2]....

Ngày tải lên: 23/03/2014, 09:20

... demonstrate the validity of this last statement, we compared the performance of a simple integrator for the ZDP 1 -reduced PTS system against that of a state -of- the- art integrator for the full ... benefit from the analysis of mathematical models of those systems. This analysis is essential for understanding the basis of the functional properties that the...

Ngày tải lên: 18/02/2014, 06:20

... present in the hemolymph shortly before pupa- tion (Fig. S2), it is possible that this peptide also serves as a growth factor for the developing appendages. Therefore, we tested the effect of the peptide ... measuring BrdU incorporation and cell number. When genital disks were cultured for 24 h with or without 8K-BLP and then pulse-labeled for 2 h with BrdU, many more ce...

Ngày tải lên: 18/02/2014, 13:20

... mediated by global conformational changes in the protein, could be a further factor in preventing the formation of misacyl- ated tRNA. For arginyl-tRNA synthetase, rearrange- ment of the enzyme active ... affinity of the soybean synthetase for canavanine (69 lm) compared with that of the jack bean enzyme (1.3 mm), and the corresponding k cat ⁄ K M values result in dis...

Ngày tải lên: 18/02/2014, 13:20

... cDNA was used for PCR performed with the gene-specific primer pairs described in Table 1. The PCR program consisted of 30 cycles of 94 °C for 30 s, 60 °C for 30 s, and 72 °C for 45 s, with a final ... specific for ERE1 and ERE2 of the HOXC13 promoter. (B) Interac- tion of MLL3 with ERs. JAR cells were treated with 100 n M E2 for 6 h before being harvested for...

Ngày tải lên: 18/02/2014, 14:20

... ATCC12633: preincubation at 94 °C for 1 min and then 30 cycles between 98 °C for 20 s and 68 °C for 3 min and finally at 72 °C for 10 min. The PCR product was digested with NdeI and HindIII and ligated into ... NE) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan, by Grant- in-Aid for Encouragement of Young Scientists 15780070 (to HM) from...

Ngày tải lên: 19/02/2014, 16:20