... cyste- ine protease, legumain, because of removal of the key amino acid in the legumain-binding site of cysta- tin C [22]. The amino acid substitutions reside in opposing parts of the cystatin C ... curve. Papain and cathepsin B inhibition The cysteine protease inhibitory capacity in cell lysates was determined by measuring the inhibition of papain as well as cathepsin B. To...
Ngày tải lên: 16/03/2014, 06:20
... of syntactic analysis of Estonian using Constraint Grammar. Constraint Gram- mar framework divides parsing into two different modules: morphological disam- biguation and determination of ... the tags attached during the last stage of the analysis indicate its syntactic functions. The underlying principle in determin- ing both the morphological interpretation and the synt...
Ngày tải lên: 24/03/2014, 03:21
Tài liệu Báo cáo khoa học: Cytokinin oxidase/dehydrogenase genes in barley and wheat docx
... differences in the cytokinin content of 7- and 14-day-old plants. pH optimum and substrate specificity of barley CKX The effect of pH on the activity of recombinant HvCKX2 and CKXs from grain, root and ... chmu ¨ lling, T. (2003) Cytokinin-induced upregulation of cytokinin oxidase activity in tob acco includes changes in enzyme glycosylation and secretion. Physiol. Pl...
Ngày tải lên: 19/02/2014, 16:20
Tài liệu Báo cáo khoa học: Role of Kupffer cells in pathogenesis of sepsis-induced drug metabolizing dysfunction pptx
... con- centrations of tumor necrosis factor-a and interleukin-6, and increased hepatic mRNA levels of tumor necrosis factor-a and interleukin-6 were decreased by depletion of KCs. Our findings suggest ... lead- ing to hepatotoxicity in liver injury, including endotox- emia and ischemia ⁄ reperfusion injury [14]. In our studies, we employed GdCl 3 to inactivate KCs based on the...
Ngày tải lên: 14/02/2014, 18:20
Tài liệu Báo cáo khoa học: Interruption of triacylglycerol synthesis in the endoplasmic reticulum is the initiating event for saturated fatty acid-induced lipotoxicity in liver cells pdf
... Exposure of a variety of cell types, including hepatocytes, to long-chain saturated fatty acids (SFAs) led to increased expression of proin- flammatory cytokines, inhibition of insulin signaling, induction ... 48 h of treatment and subsequent chromatin staining with Hoechst 33342. (B) Quantification of the percentage of apoptotic nuclei (condensed and fragmented) by random cou...
Ngày tải lên: 14/02/2014, 22:20
Tài liệu Báo cáo khoa học: Role of ceramide kinase in peroxisome proliferatoractivated receptor beta-induced cell survival of mouse keratinocytes ppt
... cultured human keratinocytes [14]. In this study, we examined the interaction between CerK and PPARb, and its role in regulating keratino- cyte survival. We report that in a mouse keratinocyte cell line upregulation ... CerK results in decreased levels of intracellular Cer, and the inhibition of stress-induced cell death. The interaction between CerK and PPARb may play an...
Ngày tải lên: 18/02/2014, 18:20
Tài liệu Báo cáo khoa học: Mechanism of dihydroneopterin aldolase NMR, equilibrium and transient kinetic studies of the Staphylococcus aureus and Escherichia coli enzymes docx
... measured the binding of HP in the absence of GA. NP and MP are the oxidized forms of the pterins, with a double bond between C7 and N8 instead of a single bond as in the reduced pterins (DHNP and DHMP). ... MP, and HPO are all DHNA inhibitors. The binding of the inhibitors to the enzymes cause a decrease in their fluorescence intensities. The increasing fluorescence inte...
Ngày tải lên: 19/02/2014, 00:20
Tài liệu Báo cáo khoa học: Inhibition of pneumococcal choline-binding proteins and cell growth by esters of bicyclic amines pptx
... pathol- ogies. Abbreviations CBM, choline-binding module; CBP, choline-binding protein; CBR, choline-binding repeat; DEAE, diethylaminoethanol; MIC, minimal inhibitory concentration; t m , midpoint of thermal transition. 364 ... changes of C-LytA upon binding of these alkaloids are different to those induced by choline, suggesting a different mode of binding. In vitro inhibi- tion a...
Ngày tải lên: 19/02/2014, 05:20
Tài liệu Báo cáo khoa học: Roles of the human Rad51 L1 and L2 loops in DNA binding doc
... within the L1 loop with alanine, a short side chain amino acid, significantly decreased the DNA-binding ability of human Rad51, without affecting the protein fold- ing or the salt-induced, DNA-independent ... significant defects in ssDNA binding and dsDNA binding A B Fig. 3. The DNA binding activities of the Rad51 mutants. (A) The ssDNA binding experiments. The /X174 circular ssDNA (40...
Ngày tải lên: 19/02/2014, 06:20
Báo cáo khoa học: Roles of conserved arginines in ATP-binding domains of AAA+ chaperone ClpB from Thermus thermophilus pptx
... exam- ine the roles of the conserved arginines, Arg322 (P2) and Arg323 (P1) of AAA-1, and Ar g747 (P1) of AAA- 2, and determined that the arginines of TClpB are not involved in nucleotide binding ... these three arginines in TClpB. By combining an arginine mutation with the WalkerA mutation in the other AAA+ module, we tested the roles of the arginines in ATP binding...
Ngày tải lên: 06/03/2014, 00:20