Báo cáo khoa học: "The use of crossreactive monoclonal antibodies to characterize the immune system of the water buffalo (Bubalus bubalis)" pdf
... The The use of crossreactive monoclonal antibodies to characterize the immune system of the water buffalo (Bubalus bubalis)105 Table 1. Monoclonal antibodies reactive with bovine and water buffalo ... The use of crossreactive monoclonal antibodies to characterize the immune system of the water buffalo (Bubalus bubali...
Ngày tải lên: 07/08/2014, 15:20
... cf. Luhn (1958)). Then, the weight of each cluster is calculated by dividing the square of the number of significant words in the cluster by the total number of words in the cluster. Sentences ... to compute the weight of a sentence, the weights of all clusters in that sentence are added. The higher the weight of a sentence, the higher is its ranking...
Ngày tải lên: 17/03/2014, 06:20
... fraction of the cost of the clinical trial. But the cheapness of (1) and (2) were partially due to the fact that they were too small to produce sta- tistically solid findings, and the cheapness of (2) and ... interested in theoretical instead of applied hypotheses. But if you want to test an applied hy- pothesis about the effect of an NLG system on hu- man users...
Ngày tải lên: 23/03/2014, 19:20
Báo cáo khoa học: Combined use of selective inhibitors and fluorogenic substrates to study the specificity of somatic wild-type angiotensin-converting enzyme docx
... validates the use of selective inhibitors as convenient tools to study the spe- cificity of the somatic form of ACE. Thus, with this approach, the catalytic property of each domain towards the hydrolysis ... were applied to the atomic position of several sets of atoms. The harmonic constants were set to 100, 5 and 0.5 kcalÆ mol )1 ÆA ˚ )2 for the ions and th...
Ngày tải lên: 16/03/2014, 13:20
Tài liệu Báo cáo khoa học: High-affinity ligand binding by wild-type/mutant heteromeric complexes of the mannose pptx
... mini-receptors was measured to assess whether the presence of the mutant mini-recep- tors affected PMP-BSA binding to the wild-type mini- receptors (Fig. 5C). In these experiments, the null hypothesis ... soluble forms of the receptor [25,26,34], indicates that the ectodomain of the receptor is capable of dimer forma- tion in the absence of ligand. Affinity cross-...
Ngày tải lên: 18/02/2014, 08:20
Tài liệu Báo cáo khoa học: Top-down MS, a powerful complement to the high capabilities of proteolysis proteomics pdf
... been found to be specific and potent HAD inhibitors. To check for covalent modifications of the enzyme, the effect of the inhibitor on the molecular mass value of HAD was measured; instead of an adduct ... substitution; MS ⁄ MS will then show the substituent positions of dif- ferent isomers. A problem for MS ⁄ MS of either the peptides for the bottom-up approach or...
Ngày tải lên: 18/02/2014, 16:20
Tài liệu Báo cáo khoa học: Casein kinase 2 specifically binds to and phosphorylates the carboxy termini of ENaC subunits ppt
... electrophoretic mobility of the phosphorylating kinase corresponds to that of the a subunit of CK2 (Fig. 9). However, other experiments aiming to detect the b subunit of CK2 in the active cytosolic fraction ... corresponded to 10% of the total protein, i.e. incorporation of 32 PintobS631 is not residual. The K m for phosphorylation of bS631 was estimated to...
Ngày tải lên: 21/02/2014, 03:20
Báo cáo khoa học: Metabolomics, modelling and machine learning in systems biology – towards an understanding of the languages of cells potx
... signalling molecules themselves but on the structures that the cell uses to detect them. A forward look By concentrating on a restricted subset of issues within the confines of a single lecture, many topics had to ... individual software elements appear to – and indeed do ) work together transparently [201], such that ‘only’ the software ‘glue’ needs to be written, somewhat...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Copper-containing nitrite reductase fromPseudomonas chlororaphis DSM 50135 Evidence for modulation of the rate of intramolecular electron transfer through nitrite binding to the type 2 copper center pot
... center of the enzyme. These modifications may be attributed to either the presence of the substrate and/or a product in the vicinity of the T2Cu active center rather than to disruption of this ... CoCl 2 ,0.6andNa 2 B 2 O 7 , 0.1. The optical density, the concentrations of nitrate [17] and nitrite [18], the pO 2 and the pH of the medium were monitored, and...
Ngày tải lên: 07/03/2014, 15:20
Báo cáo khoa học: Saccharomyces cerevisiae Ybr004c and its human homologue are required for addition of the second mannose during glycosylphosphatidylinositol precursor assembly ppt
... been implicated in all but one of the predicted reactions in the GPI pathway. The exception is the addition of the second mannose to the GPI core. We show here that depletion of the essential, multispanning ... leads to the bio- chemical defects expected if addition of the second, a-1,6-linked mannose to GPI precursors is prevented. These defects are a block in...
Ngày tải lên: 07/03/2014, 16:20