Báo cáo khoa học: "Tissue distribution of bovine viral diarrhea virus antigens in persistently infected cattle Taekyun Shin* and Helen Acland1" ppt

... enrichment in iNOS activity within the particulate fraction of epithelial cells. Apical distribution of iNOS in intestinal epithelial cells To get more insight into the localization of iNOS in intestinal ... [18]. Results Subcellular distribution of iNOS protein and activity in vitro The distribution of iNOS protein in the cytosol and particulate fraction was exa...

Ngày tải lên: 19/02/2014, 16:20

... tropomyosin and actin, the IANBD probe retained its typical responses to changes in Ca 2+ and S1 binding (Fig. 6). Fitting binding curves to obtain binding parameters is difficult in the case of Ca 2+ because ... the measured effects of Ca 2+ on binding in the presence of ATP [35,36], equilibrium binding in the presence of ADP [16], binding kinetics [15,37] and the genera...

Ngày tải lên: 07/03/2014, 09:20

... (pseudo-coloured green). C, G andKaremergedimagesfromAandB,EandFandIandJ,respectively.AnenlargementoftheboxedareasinC,GandKareshowninD,Hand L, respectively. Images shown in this figure are single xy confocal ... microdomains (GEM) of the plasma membrane and to be involved in the regulation of epidermal growth factor receptor (EGFr or ErbB1) activity. To gain further insight into the...

Ngày tải lên: 16/03/2014, 16:20

... by a machine and they are of limited variety, so that their listing in a dictionary is practicable. From the practical point of view, the distri- bution is useful in planning input and storage ... not shared by the others, of being concentrated at relatively low values of length, and of having no elements exceeding a certain length (Fig. 1). Words, defined in this...

Ngày tải lên: 16/03/2014, 19:20

... 1). Results Binding of AMA and odorants in solution The dissociation c onstants of the AMA–OBP c omplexes fo r the bovine and porcine OBP isoforms were, respectively, 1 .0 and 1.5 l M and the corresponding ... onformational changes involving protein loops or backbone. T hus, the role of OBP in signal triggering remains unresolved. 3 Keywords: crystal str ucture; domain swa...

Ngày tải lên: 30/03/2014, 15:20

... strains of Japanese encephalitis virus, 28 strains of St Louis encephalitis virus, 172 strains of West Nile virus and 24 strains of yellow fever virus using BLASTn [22]. The genome sequences of the flaviviruses ... to dengue virus when computationally tested against 291 strains of other nondengue flaviviruses, including strains of Japanese encephalitis virus, St. Louis...

Ngày tải lên: 14/02/2014, 19:20

... counterstaining. Haematoxylin–eosin staining Haematoxylin–eosin staining was carried out following the routine histological method described by Kayser and Bubenzer [38]. The haematoxylin–eosin staining technique employs ... KH 2 PO 4 and 8 mm Na 2 HPO 4 , and kept at 37 °C for 15 min in a beaker containing NaCl ⁄ P i . The intestine was then filled with a solution containing 1.5 mm EDT...

Ngày tải lên: 18/02/2014, 16:20

... determine the calcium-binding domain of the protein. (A) Schematic drawing of the recombinant proteins. Six GST-fused recombinant proteins containing the three distinctive domains of tandem repeat 1 ... Of these recombinant proteins, rOMM-64-I, III, IV and V, which include the Glu-rich domain, were found to have calcium-binding activity. rOMM-64-II and -C and GST were stained re...

Ngày tải lên: 18/02/2014, 17:20

... DNA and AdoHcy(AdoMet) [44], M.HhaI consists of two domains, the large domain containing the S-adenosyl-l-methionine (AdoMet) binding site and the catalytic center, and the small domain contain- ing ... CGM and G CX + ⁄ CGM), 5-methylcytosine was introduced into one of the strands of the recognition site instead of the target cytosine. The melting curves of the hemi- methyla...

Ngày tải lên: 19/02/2014, 00:20

... staining of 293-VnR and ATDC5 cells expressing the Y373C mutant with anti- FGFR3 and anti-phosphotyrosine sera showed both intracellular and cell-surface phosphotyrosine staining (Figs 2Eb,c and ... Conversion of Lys650 into methionine in the TK2 domain induces hyperphosphorylation and marked intracellular retent- ion of the mutant receptor leading to phosphorylation of ta...

Ngày tải lên: 19/02/2014, 00:20