Báo cáo khoa học: "Detection of canine distemper virus (CDV) through one step RT-PCR combined with nested PCR" ppt

... and PCR. We established a one- step RT-PCR. Moreover, a nested PCR was developed from the product of one- step RT-PCR. Sensitivity of the one- step RT-PCR combined with nested PCR increased hundredfold ... of one- step RT-PCR and nested PCR. Lanes 1- 4 of panel A: amplified products with one- step RT-PCR using virus titier 10 3 to 10 0 PFU/ml an...

Ngày tải lên: 07/08/2014, 14:23

... canine UCB-MSCs may have enhanced new bone formation. Analyses of bone marrow MSCs with real-time polymerase chain reaction and of bone marrow MSC-conditioned medium by antibody-based protein ... the implantation of canine UCB-MSCs mixed with β-TCP in bone defect model dogs, and the successful repair of a non-union fracture case by allografting and injection of canine...

Ngày tải lên: 07/08/2014, 23:22

... recommendations. Briefly, 250 μL of the homogenated samples was mixed with 750 μL of TRIzol and incubated for 15 min at room temperature. Following the addition of 200 μL of chloroform, the mixture was ... chain reaction assays for the diagnosis of infection with the porcine endogenous retrovirus and the detection of pig cells in human and nonhuman recipients of pig xe...

Ngày tải lên: 07/08/2014, 23:22

... suffer from ho- mophone errors caused by incorrect choices. Care- lessness of choice alone is not the cause of homo- phone errors; Ignorance of the difference among homophone words is serious. ... problem of choosing the correct word from the homophone set the homo- phone problem. In order to detect homophone errors, we make a list of homophone sets in ad- vance, find a ho...

Ngày tải lên: 22/02/2014, 03:20

... (%), recall (%), and F1 (%) of (dis)agreement detection using manual annotations with- out and with prosodic features. Agreement P R F1 w/o prosodic 81.5 43.2 56.5 with prosodic 81.8 44.0 57.2 Disagreement P ... In Proceedings of HLT/NAACL. D. Hillard, M. Ostendorf, and E. Shriberg. 2003. De- tection of agreement vs. disagreement in meetings: Training with unlabeled data. In Pro...

Ngày tải lên: 07/03/2014, 22:20

... for chunking Tag Explanation of tag B Beginning of a clause E End of a clause I Interior of a clause (except B and E) O Exterior of a clause S Clause consisting of one bunsetsu boundaries can ... includes tran- scriptions of the speeches as well as audio record- ings of them. Approximately one tenth of the speeches in the CSJ were manually annotated with various kinds...

Ngày tải lên: 23/03/2014, 18:20

... index h [17,20], which is defined as the variance of GC content of the region divided by that of the whole genome. If h ( 1, the variance of GC content of the region may be small enough to be considered ... the range of 0.06–0.67, which are higher than those of the isochores. As can be seen, the difference of GC content between two adjacent regions are relatively small, usu- al...

Ngày tải lên: 30/03/2014, 20:20

... template; Lane 3 : 1 ng of template; Lane 4 : 100 pg of template; Lane 5 : 10 pg of template. Table 1. Comparison of PCR assay with conventional methods for the detection of PPE caused by L. ... cycles. Detection of Lawsonia intracellularis in diagnostic specimens by one- step PCR 37 examination for the thick intestines of slaughter pigs was sensitive, but not specific...

Ngày tải lên: 07/08/2014, 14:22

Ngày tải lên: 07/08/2014, 15:20

... specialist participation with practical objectives [13, 14]. With this in mind, the presence of heavy metals and pollutants in mineral mixtures is quite important today where epidemics of animal-related ... agricultural industry of many countries. Animal feed has become a concern in animal health, and the safety of products is the main objective of this study. Due to the high nu...

Ngày tải lên: 07/08/2014, 15:20