... frag-ments containing a complete x -5 gliadin gene, oligonucleo-tides, 5 ¢-AAGTGAGCAATAGTAAACACAAATCAAAC-3¢and 5 -CGTTACATTATGCTCCATTGACTAACAACGATG-3¢, were constructed based on fragment DNA sequencesof ... Foster City, CA, USA).Expression and purification of recombinantproteinSense (5 -ATTTCATATGCAACAACAATTCCCCCAGCAACAATCA-3¢) and antisense (5 -TCTCGGATCCTCATAGGCCACTGATACTTATAACGTCGCTCCC-3¢) ... residue in x- 5a gliadin are compatible with thestructural features of x -5 gliadin. Kasarda et al. repor-ted that the N-terminal amino acid sequence ofx -5 gliadin from wheat (T. aestivum ‘Justin’)...