... sensitivity. In this study, the effect of NaCl concentration on the kinetic constants and thermodynamic stability of VsEndA and VcEndA was investigated. In addition, the effects of reciprocal mutations of ... mayreflect the dependence of kcat on the substrate binding and dissociation rate constants, as found in the coldadaptation of cod trypsin [39], or, in the case of VsEndA, may be linked in some ... extremophilicproteins has so far focused on the obvious, i.e. the effects of temperature on thermophilic and psychro-philic proteins, and salinity on halophilic proteins.However, many of the environments in...