... membrane. The way this contact takes place and the molecular features of the protein involved are yet to be deciphered. The lack of information about the mode of action and the availability of a ... (i.e. the b-hairpin loop and part of the adjoining b-sheet) and Ôresidues in the loop connecting b-strand and a- helix and contiguous residues on the...
Ngày tải lên: 20/02/2014, 11:20
... proteases (Fig. 1). The catalytic triad residues Asp113, His57, and Ser194 in API are placed at an identical location to those of chymotrypsin and bovine trypsin. The catalytic triad residues and ... typically W169V and W16 9A, deviates from the original position. In the struc- tural deviation, the solvent ASA of the side-chain of His210 increased with the decr...
Ngày tải lên: 21/02/2014, 03:20
Báo cáo khoa học: Critical role of the plasma membrane for expression of mammalian mitochondrial side chain cleavage activity in yeast pptx
... (5¢-atagacacgcaaacacaaatacaca cactaaattaataatgaccggatcATGTACTTCCCCTTTTTAGG CAGAT-3¢) and lip2 (5¢-cagtagagacatgggagatcccccgcgg aattcgagctcggtacccgggTCATTCTTTATTTAGAGCATC CAGC-3¢). The sequences in lower-case ... was generated by disrupting the ATF2 gene of CA1 0 with the KanMX4 cassette, which confers G418 resistance. Primers 5¢ATF2-Kan 5¢-AGACTTTCAAACGAATAATAACTT CAGCAATAAAAATTG...
Ngày tải lên: 08/03/2014, 08:20
Báo cáo khoa học: Essential role of the C-terminus in Melanocarpus albomyces laccase for enzyme production, catalytic properties and structure pdf
... efficiency, indicating that the last resi- due, Leu559, has a role in the catalysis of ABTS oxida- tion. The catalytic constants for the phenolic substrates 2,6-DMP and syringaldazine were not greatly affected by ... suggested that the secondary structure of the mutant was not significantly altered. Even a single amino acid change in the C-terminus of MaL (L55 9A...
Ngày tải lên: 16/03/2014, 00:20
Báo cáo khoa học: Dual role of Nbs1 in the ataxia telangiectasia mutateddependent DNA damage response ppt
... contains a forkhead-associated (FHA) domain and a Brca1 COOH-terminus (BRCT) domain in its NH 2 -terminal region, as well as an Mre11-binding domain and an ATM-binding domain in its COOH- terminal ... of DNA strand breakage and in the activation of the ATM-dependent DNA damage signaling path- way [6,34–36]. However, normal activation of the kin- ase activity of A...
Ngày tải lên: 16/03/2014, 13:20
Báo cáo khoa học: Functional role of the linker region in purified human P-glycoprotein pot
... basal and substrate-stimulated ATPase activity than native P-gp (Fig. 2 and Table 2). These results indicate that, as for the native P-gp, the N-half and the C-half fragments of the linker-cleaved ... proteases at 20 °C for 1.5 h. The cleavage was stopped and ATPase activity was measured as indicated in the Materials and methods. All data represent the means ± SD f...
Ngày tải lên: 29/03/2014, 23:20
Báo cáo khoa học: Functional role of Bb-chain N-terminal fragment in the fibrin polymerization process pdf
... in the N-terminal region of the Aa-chain by the splitting off of fibrinopeptide A [6]. Site a is formed by amino acid residues cGln329, cAsp330, cHis340 and cAsp364, situated in the cC-domain ... in the fibrin bC-domain [7]. The ‘B’–‘b’ pairing leads to structural rearrangements in the D-domain and the strengthening of protofibril lateral contacts [9,10]. Many...
Ngày tải lên: 30/03/2014, 03:20
Báo cáo khoa học: "the Role of Non-Timber Forest Products (NTFPs) in Livelihood Strategies and Household Economies in a Remote Upland Village in the Upper Ca River Basin, Nghe An, Vietnam" pdf
... UNIVERSITY OF AGRICULTURE The Role of Non-Timber Forest Products (NTFPs) in Livelihood Strategies and Household Economies in a Remote Upland Village in the Upper Ca River Basin, Nghe An, Vietnam ... gain cash income from timber and agricultural products. Livelihood Strategies of Households in Tha Lang Hamlet In this anal...
Ngày tải lên: 06/08/2014, 19:20
Báo cáo khoa học: Biological role of bacterial inclusion bodies: a model for amyloid aggregation potx
... software such as aggrescan [42] or tango [43]. Protein aggregation can be understood as an anoma- lous type of protein–protein interaction. As for native interactions, the attainment of ordered aggregated structures ... use of these underesti- mated bacterial aggregates as intriguing models for the analysis of protein–protein interactions in the context of amyloid and prio...
Ngày tải lên: 06/03/2014, 00:20
Báo cáo khoa học: Dual role of oxygen during lipoxygenase reactions potx
... 2523 of the hydrocarbon chain, via epoxidation or dimeriza- tion [24]. (b) Escape of the catalytically inactive ferrous LOX from the catalytic cycle. When the catalytically active ferric LOX catalyses ... the absorbance at 275 nm during the time course of the reaction. From Fig. 6Aa it can be seen that there was a linear increase in absorbance at 275 nm and subsequen...
Ngày tải lên: 16/03/2014, 19:20