Báo cáo sinh học: " Regulators of kinesin involved in polarized trafficking and axon outgrowth" potx

... the defects of Drosophila kinesin mutants in axonal transport, suggesting that UNC-76 coordinates with kinesin to regulate cargo trafficking in axons [23]. The similarity of the unc-69 and unc-76 ... is involved in normal axon development. By interacting with UNC-76, UNC-69 is likely to be tethered to kinesin complexes, and it may coordinate with UNC-76 and kinesin...

Ngày tải lên: 06/08/2014, 18:21

... Notch [16]. In ofut1 mutant cells, the Notch protein accumulates in intracellular compartments marked by ER markers, and knockdown of Ofut1 using double- stranded RNA in cultured cells inhibits ... the endo- plasmic reticulum (ER), in Notch-ligand binding and in endocytic trafficking of Notch. These different activities have been incorporated into two distinct models (Figu...

Ngày tải lên: 06/08/2014, 18:21

... approved for in- service training in the ministry by the training coordinators. They believed that favouritism seemed also to exist with regard to both continuous education and in- service training. An ... impact on infant and maternal mortality. A range of factors, including worsening socioeconomic conditions in much of sub-Saharan Africa, increasing mobility and migration o...

Ngày tải lên: 18/06/2014, 17:20

... For infection of WR, BSC-40 cells were maintained in infection media (EMEM) supple- mented with 5% FCS, 2 mM glutamine, and 10 mM gen- tamicin sulfate and were infected at a multiplicity of infection ... by stain- ing with Coomassie R-250 and de-staining until protein bands could be easily visualized. Protein bands of interest were excised in as small of piece of gel as po...

Ngày tải lên: 18/06/2014, 18:20

... combination of both methods greatly speeds up ascer- taining when a NoV outbreak is occurring and which strain is responsible. A SYBR green mastermix containing a proof reading enzyme would again ... obtained. (C) Melting curve of GI standards showing melting point at 90°C in descending order 10 7 – 10 1 molecules. A B C Table 1: Primers and probes used for Lightcycler RT-...

Ngày tải lên: 18/06/2014, 18:20

... labelling The cells were infected for 3 h and maintained in RPMI containing 5% FCS for 21 or 69 h. The cells were washed with PBS and incubated for 30 min in low-methionine DMEM medium (3.0 µg of ... After 3 h incubation, cells were washed and maintained in RPMI containing 10% FCS for 24 h. The cells were washed and RPMI containing 2% FCS was added and incubation continued....

Ngày tải lên: 18/06/2014, 18:20

... quality of the Patlak images and could be further investigated to improve the accuracy of automated tumor delineation. Despite the lack of using such a denoising method, our results were in line ... the data interpretation, implemented some of the tumor delineation methods, and assisted in drafting the manuscript. MY implemented some of the tools to perform tumor delinea...

Ngày tải lên: 18/06/2014, 18:20

... differences in infection to differential virus binding, internalization or gene expres- sion in different PBL cell lineages. In any event, the conse- quences of virus tropism in the pathogenicity of poxviruses ... vaccination are a critical issue in the event of mass vaccination, understand- ing the basis of MVA attenuation may lead to the develop- ment of better vaccine v...

Ngày tải lên: 18/06/2014, 22:20

... depending on their cost. Overall, it appears that selected single chain antibodies are promising candidates for interfering with binding of gD to HVEMs and studies in a guinea pig model of GH suggest ... infection in vitro, inhibits cell-to- cell spread of virus and can be used to prevent infection in a guinea pig model of GH. Results Construction and expression of singl...

Ngày tải lên: 18/06/2014, 22:20

... using random hexadeoxynucleotides. Reverse transcriptions (RT) were performed in a 20 µl volume containing 5 µl of eluted RNA, 4 µl of 5x RT buffer, 0.5 mM of each deoxynucleotide, 0.5 µg of random ... carried out in a 100 µl volume containing 10 µl of the reverse transcription product, 0.2 mM of each dNTP, 400 nM of genomic primer and 400 nM of antigenomic primer and...

Ngày tải lên: 18/06/2014, 22:20