Báo cáo sinh học: "ERK2 mitogen-activated protein kinases affect Ras-dependent cell signaling differentially" pps

... KIAA0247- expressing cells could be crucial in controlling the G2/ M c heckpoint and in decreasing the number of cells in G2/M. At the same time, reduced levels of cyclins woul d negatively control the cell ... p53-null HCT116 cells (HCT116 p53 -/- ) with varying KIA0247 expression stained with propidium iodide for evaluation of nuclei fluorescence. The percentages of cell numbers in the...

Ngày tải lên: 18/06/2014, 19:20

... 14.13 Journal of Biology 2006, 5:14 Research article ERK1 and ERK2 mitogen-activated protein kinases affect Ras-dependent cell signaling differentially Chiara Vantaggiato ¤*† , Ivan Formentini ¤* , ... 41-/43-kDa mitogen-activated protein kinase signaling pathway in human tumors. Oncogene 1999, 18:813-822. 12. Milella M, Kornblau SM, Andreeff M: The mitogen-activated pr...

Ngày tải lên: 06/08/2014, 18:21

... Regulated cellular partitioning of SR protein- specific kinases in mammalian cells. Mol Biol Cell 17, 876–885. 5 Tang Z, Yanagida M & Lin RJ (1998) Fission yeast mitotic regulator Dsk1 is an SR protein- specific ... unable to reenter the cell cycle. Aberrant cell cycle activation provokes neuronal cell death, whereas cell cycle inhibition increases neuronal survival. SRPK2 tri...

Ngày tải lên: 06/03/2014, 01:20

... non-opti- Calnexin interacts with the HPV16 E5 protein in cellular extractsFigure 4 Calnexin interacts with the HPV16 E5 protein in cellular extracts. HEK-293T cells were transfected with AU1-tagged ... known to allow for increased protein expression of the protein in eukaryotic cells [46-48]. HEK-293T cells were transfected with the codon-adapted E5-coding DNA and protein expressio...

Ngày tải lên: 18/06/2014, 18:20

... For the luciferase reporter assays, 293FT cells were cultured in 24-well plates at a cell density of 1.5 × 10 5 cells per well. Twelve hours later, cells were transfected with a control plasmid ... rotavirus infected cells. MA104 cells were infected with rotavirus SA11 at a m.o.i. of 0.1. Cell extracts were prepared at 0, 4, 8, 12, 24 and 36 h post-infection (p.i). RIG-I protein le...

Ngày tải lên: 18/06/2014, 18:20

... Bonini C, Naldini L, Brambilla R: ERK1 and ERK2 mitogen-activated protein kinases differentially affect Ras-dependent signal transduc- tion and cell growth. J Biol 2006, 5:14. 4. KiNET [http://www.kinexus.ca/kinet] 5. ... MAPK-activated protein kinases: a family of protein kinases with diverse biological functions. Microbiol Mol Biol Rev 2004, 68:320-344. 2. Petak I, Hought...

Ngày tải lên: 06/08/2014, 18:21

... heteroge nous popula- tion of hematopoietic and mesenchymal stem cells, endothelial progenitor cells and immature immunologi- cal cells [16,20]. In this study, CBMC transplantation wasexaminedasapotentialtherapyforhereditary ataxia. ... Comi GP, Corti S: Stem cell therapy in stroke. Cell Mol Life Sci 2009, 66:757-772. 9. Liang J, Zhang H, Hua B, Wang H, Wang J, Han Z, Sun LY: Allogeneic m...

Ngày tải lên: 18/06/2014, 19:20

... quantified. Defining Cell Sensitivity An analysis of cell line sensitivity to GSK1070916 was per- formed with the data generated from screening cell lines in cellular proliferation assays and from cell cycle ... incubation. For resistant cell lines, however, poly- ploid cell populations were tolerated over time and sig- nificantly less cell death was observed. To maintain genome...

Ngày tải lên: 18/06/2014, 22:20

... cytotoxicity occurred in mGC8 cells and MCA310 cells to a similar level (Figure 3C). In contrast, LN cells from mGC8 GM- CSF/IFA-treated mice induced sp ecific lysis of mGC8 cells at an E:T ratio of ... FoxP3 + CD25 + cells calculated as a percentage of CD4 + T cells. (B) Absolute number of CD4 + CD25 + Foxp3 + cells calculated from initial splenocyte counts. (C) Ratio of CD8 + T cells to CD4...

Ngày tải lên: 18/06/2014, 22:20

... Stromal Cells TNFalpha BM Stromal Cell Expression of VCAM-1 VLA-4- Dependent Melanoma Cell Adhesion Melanoma Cell Proliferation VEGF Receptor-Expressing Melanoma Cells BMSC and Melanoma Cell ... <0.01ascomparedwithLPS-treated BMSC-CM-treated A375M cells; #P < 0.01 as compared with rhVEGF- treated A375M cells. B16M Cell Proliferation (as1x10 3 B16Mcells/well) 0 10 15 20 25...

Ngày tải lên: 18/06/2014, 22:20