Báo cáo sinh học: "Gene expression neighborhoods" ppsx

... almost always show some alteration in expression due to insertion site - and sometimes the effect on expression is dramatic. That even subtle differences in gene expression can have consequences in ... that although expression domains reveal some sort of structural feature, only one or a few genes in the neighborhood are bona fide targets. The bottom line for any would-be gene -expres...

Ngày tải lên: 06/08/2014, 18:20

... investigation. Sequence-dependent side effects of siRNAs on gene expression are expected to be identical in different cell types. Gene expression profiles obtained for 143B, A549 and IMR-90 cells allow ... Representative kinetic profiles of gene expression in 143B cells incubated with DF (circles) or transfected with SREBF1 ⁄ siR- NA1 (squares) or the NT siRNA (triangles). Gene expressio...

Ngày tải lên: 07/03/2014, 05:20

... Biosystems). An expression vector for the putative luminal domain of At SEL-1L, which corresponds to residues 21–621, was con- structed as described below. For cloning into an expression vector, ... LIC digested with NdeI and XhoI. Expression vector pET-30 ⁄ At SEL-1L allows the fusion of the histidine tag LEHHHHHH to the C-terminus of a recombinant protein. Expression and purification...

Ngày tải lên: 07/03/2014, 21:20

... the PCA over the matrix having the expression values rel- ative to 7077 ORF (base 2 logarithm of the ratio Fig. 3. The first three modes (pc1rand1–pc1rand3) of the gene expression dynamics for the elutriation ... dynamics of gene expression that are independent from reproductive cycles, hence contradicting simple ergo- dic assumptions The aspecific character of the observed coordinated ge...

Ngày tải lên: 30/03/2014, 08:20

... division cycle 2 genes and cyclin E. The expression patterns of genes of the centromer/kinetochore complex and genes involved in DNA repair were similar to the expression patterns in SCC (Table 4). Corroboration ... have demonstrated a strong pituitary tumor-transforming gene 1 expression in SCLC, adenocar- cinomas, as well as in SCC, whilst a weak expression was only found in the lum...

Ngày tải lên: 18/06/2014, 15:20

... resistant Huh-7 cells have reduced expression of Tyk2. Reduced expression of Jak-1 was also observed in some resistant Huh-7 cells. The levels of Stat1 and Stat2 expression was found be similar ... developed have reduced expression of Tyk2 as compared to the sensitive replicon cells. In some resistant replicons expression of Jak-1 was also reduced. There were no differences in the...

Ngày tải lên: 18/06/2014, 18:20

... differentiation- dependent expression of the PV L1 proteins in KCs [8]. We next examined how pre-transfection treatment of methylcellulose affected transcriptional and translational expressions of the four PV L1 expression ... L1 protein expression was detected in HPV-infected KCs treated by methylcellulose although L1 mRNA was well transcribed [16]. In this work, we investigated effect...

Ngày tải lên: 18/06/2014, 18:20

... protein expression. The log-rank test was used to calculate p values. Table 1 Protein expression of CDK4 between lung cancer and normal lung tissues Group Protein expression P value Cases High expression ... reduction of endogenous CDK4 expression markedly elevated the expression level of tumor suppressor p21 (≥2 folds). Further, we confirmed the upregulated pro- tein expression...

Ngày tải lên: 18/06/2014, 19:20

... gene expression was unsatisfied [4]. To prevent the toxicity of suicide genes in normal cells, tumor specific promoters including hTERT promoter have been utilized to drive the specific expression ... and human A549 cell lines at indicated MOIs. B, HRP expression of LLC cells in various treatment groups was analyzed by western blot. C, IL-12 expression in the supernatant of LLC cells...

Ngày tải lên: 18/06/2014, 19:20

... cancer patients. Expression of EGFR family members is highly regu- lated, and outside of the bon e marrow, expression is generally low, with increased expression in tumors commonly characterized as over -expression, ... Her2 expression showed uniform membranous form. In primary tumor tissues, high membranous Her2 expression level was found in 104 (26.9%) cases while positive membran...

Ngày tải lên: 18/06/2014, 22:20