... each substrate: mutan (a-1,3- and a-1,6-glu- can), nigeran (a-1,3- and a-1,4-glucan), soluble starch (a-1,4- and a-1,6-glucan), pustulan (b-1,6-glucan), laminarin (b-1,3-glucan), carboxymethilcelullose ... a-1,3-glu- cans with a-1,6 branches. The observed optimum pH and thermostability are in the range obtained for the T. harzianum CECT2413 mutanase [25] and other enzymes from T. harzianum CC...
Ngày tải lên: 19/02/2014, 16:20
... Software, San Diego, CA). Acknowledgements This work was supported by grants from the Heart and Stroke Foundation of Canada (grant T4134) and the Canadian Institutes of Health Research (grant MT11270). ... Recombinant iron-regulatory factor functions as an iron-responsive- element-binding protein, a translational repressor and an aconitase. A functional assay for translation repres- sion...
Ngày tải lên: 07/03/2014, 09:20
Báo cáo khoa học: "Experience with an Easily Computed Metric for Ranking Alternative Parses" pptx
... modified and extend- ed to work over entire sentences and to provide for top-down computation. Also, we have done an analysis of 80 sentences with multiple parses from our data base to evaluate ... improvement in performance from June to December can be attributed both to writing additional grammar rules and to relaxing overly restrictive conditions in other rules. It can...
Ngày tải lên: 08/03/2014, 18:20
Báo cáo khoa học: "EXPERIENCES WITH AN ON-LINE TRANSLATING DIALOGUE SYSTEM" docx
... parser, semantic analyzer, and target sentence generator. Introducing a full-fledged semantic analyzer con- flicts with avoiding increases in processing time and memory use. To resolve this ... main role is to separate parsing from semantic analysis, i.e., to make these processes independent of each other. In LTNG, parsing includes no semantic analysis. Any ambiguities in an i...
Ngày tải lên: 08/03/2014, 18:20
Báo cáo khoa học: "Parsing with an Extended Domain of Locality" pot
... potentially substantial benefits to parsing efficiency if all de- pendencies that the parser is checking can be lo- calized with the EDOL. It is tOO early to say how best to make the trade-off, ... apply to other lan- guages is outside the scope of the present work. 5 Acknowledgements This work is supported by UK EPSR.C project GR/K97400 and by an EPSRC Advanced Fellow- shi...
Ngày tải lên: 08/03/2014, 21:20
Báo cáo khoa học: Shewasin A, an active pepsin homolog from the bacterium Shewanella amazonensis pptx
... western blot analysis with an antibody against His tag (not shown). The purified recombinant shewasin A was subjected to analytical size-exclusion chromatography (Fig. 2C) under nondenaturing conditions and ... Purification and analysis of recombinant shewasin A. Wild-type shewasin A was produced in E. coli in soluble form, fused to an N-terminal His-tag. (A) HisTrapHP chromatogram. Rec...
Ngày tải lên: 14/03/2014, 22:20
Báo cáo khoa học: Optimization of an Escherichia coli system for cell-free synthesis of selectively 15N-labelled proteins for rapid analysis by NMR spectroscopy pdf
... I sotope Laboratories (Andover, MA, USA). L -[ 15 N]Alanine, L -[ 15 N]leucine and L -[ 15 N]threonine were obtained from Spectra Stable Isotopes (Columbia, MD, USA) and L -[ 15 N]lysine [aN] .2HCl ... 2004 Acknowledgements We thank Dr S imon Bennett for m easurements o f ESI mass spectra. K.O. and G.O. thank the Australian Research Coun cil for a CSIRO- Australian Postdoctoral and Federa tio...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo khoa học: Interaction of an 40 kDa protein from regenerating rat liver with the )148 to )124 region of c-jun complexed with RLjunRP coincides with enhanced c-jun expression in proliferating rat liver pdf
... lgeachof nRNE-d (lanes 1, 3 and 5) and rRNE-d (lanes 2, 4 and 6) and 1 ng each of labelled Jun-25 (encompassing the )148 to )124 region of c-jun, lanes 1 and 2), J un-25A (encompassing the )139 to )124 ... -d and rRNE-d with Jun-25. EMSA reactions w ere c arried outin the presence of 1 ng of rad iolab elled Jun-25 and 100 lg(lanes1,3and5)and150lg (lanes 2, 4 and 6) o f nuclear extracts. L...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo khoa học: "French TimeBank: An ISO-TimeML Annotated Reference Corpus doc
... hand. Manually elim- inating incoherencies is an arduous task, and per- forming an online coherence check during annota- tion of relations would be extremely useful in a man- ual annotation tool. ... of the total corpus tokens in this sub-genre. References ISO 2008. ISO DIS 24617-1: 2008 Language Resource Management - Semantic Annotation Framework - Part 1: Time and Events. International...
Ngày tải lên: 17/03/2014, 00:20
Báo cáo khoa học: "Redundancy Ratio: An Invariant Property of the Consonant Inventories of the World’s Languages" pdf
... Linguistics Redundancy Ratio: An Invariant Property of the Consonant Inventories of the World’s Languages Animesh Mukherjee, Monojit Choudhury, Anupam Basu, Niloy Ganguly Department of Computer Science and ... used to express the consonants. An interesting observation is that this quantitative feature-based measure of redun- dancy is almost an invariance over the consonant inventories...
Ngày tải lên: 17/03/2014, 04:20