HPLC for Food Analysis phần 6 ppt

... prerequisite for successful food analysis. The broad selection of cleanup andenrichment techniques takes into account the manymatrices and compound classes under study.Sample preparation for HPLC ... automatically. 62 5Characteristics of a good sampleintroduction deviceFigure 46 A typical 6- portinjection valveAfter the sample has been prepared for introductiononto the LC column, analysis ... rarelyused in food analysis. However, the input of modern SFEinstruments can be automated with sampling devices. Thismethod is used primarily for GC, 26, 32although LC couplingalso has been performed...

HPLC for Food Analysis phần 3 ppt

... 60 0700FB1334.4352.4370.5392.5411 .6 722.5723.50200000100000m/z200300400500 60 0700m/z200300400500 60 0700m/z 163 .1170.1220.13 36. 2354.43 76. 6750.5 769 .5728.57 06. 5707.5704.725000015000050000FB3FB23 36. 4354.5512.0553.57 06. 5707.3708.7728.5728.5Figure ... to 100V12345 6 78Time [min]9. 862 6. 2417 .67 5MS EIC m/z 7233.237FB1FB1MS EIC m/z 3353.228MS EIC m/z 707FB3FB2 6. 2487 .68 3FB3FBMS EIC m/z 3372100000 60 000200005000015000025000010000020000030000020000 60 000100000Figure ... µl/minInjection volume 1 µlColumn compartment 40 °CDetector UV-DAD 210 nm /6 nm, ref. 360 /60 nm254 nm /6 nm,ref. 360 /60 nmIonization mode API-ES positiveNebulizer pressure 50 psigDryng gas...

HPLC for Food Analysis phần 8 ppt

... 1000 0.9Furazolidone 4.54 A 16. 0 96 16. 024 16. 028 992 984 1.3Pyrazon 13.72 A 19.024 18.987 19.000 1000 1000 1.7N-Acetylsulfapyidine 14 .66 A 23.307 23.282 23.282 9 76 1000 1.1Ethopabat *up 13.40 ... is a well-establishedtechnique for food analysis, LC/MS is only now emerging asa useful tool in this area. A GC-based analysis is appropriateonly for those food compounds that are volatile ... can be monitored (figure 63 ).97Multisignal1 excitation WL at 260 nm4 emission WL at 350, 420, 440 and 500 nmEx= 260 , Em=350Ex= 260 , Em=420Ex= 260 , Em=440Ex= 260 , Em=500Ex=275, Em=350,...

HPLC for Food Analysis phần 1 potx

... . 76 Gradient formation at high pressure . . . . . . . . . . . . . . . 77Gradient formation at low pressure . . . . . . . . . . . . . . . 77VIIPart TwoThe Equipment Basics HPLC for Food Analysis A ... used for various purposes in modern food processing. For example, citric acid adds a fresh, acidic flavor, whereassuccinic acid gives food a more salty, bitter taste. Inaddition to rendering foods ... for peak identification, make gaschromatography (GC) the most popular chromatographicmethod. HPLC separates and detects at ambient temperatures. For this reason, agencies such as the U.S. Food...

HPLC for Food Analysis phần 2 ppsx

... Note 5 964 -3559E, 1995.024 6 8101214mAU24 6 81012 465 nm/30 nm 60 0 nm/40 nmPatent blueChinolin yellowTime [min]Woodruff lemonadeSpectra of different colors300 400 500 60 0 700 ... FD&C Yellow No. 6; Official Method AOAC 980.24: Sulfanilic acid in FD&C Yellow No. 6. 6. A.G. Huesgen, R.Schuster, “Sensitive analysis of synthetic colorsusing HPLC and diode-array ... µlDetector UV-DADsignal A: 254/50 nm (for optimization ofseparation)signal B: 350/20 nmsignal C: 465 /30 nmsignal D: 60 0/40 nm4. Official Methods of Analysis, Food Compositions; Additives, NaturalContaminants,...

HPLC for Food Analysis phần 4 ppsx

... amperometryChromographic conditions for electrochemical detectionThe HPLC method presented here was used for the analysis of iodide in table salt.1734 HPLC method performanceLimit of detection for ECD 40 µg/lRepeatability ... refractive index HPLC method performanceLimit of detection for ECD 50 µg/l with S/N = 2Repeatability ofRT over 10 runs < 0.3 %areas over 10 runs 5 %24 6 8mV40 60 80100120140 160 180200StandardOlive ... µlDetector UV-VWDdetection wavelength 266 nmChromatographic conditionsThe HPLC method presented here was used for the analysis of anions in drinking water.Figure 23 Analysis of anions in drinking...

HPLC for Food Analysis phần 5 docx

... have set maximumtolerance levels for these compounds in foodstuffs. HPLC isnow preferred for the analysis of amines in food matricesbecause of its shorter analysis time and relatively simplesample ... softwarecomponents needed for successful HPLC, and an introduction to the analytical techniques that have become routine in food analysis Part TwoChromatographic conditions for UV detectionThe HPLC method ... two mass spectra.543Time [min]2000040000 60 00080000100000120000140000 160 000Abundance4050 60 70 8090100110T 46 T92T15T12T14T58T60 -61 T8T42Figure 42Capillary LC-MS of a phytochrome...

HPLC for Food Analysis phần 7 pot

... performaccording to food analytical requirements. The former has asingle dual-piston mechanism for low-pressure gradientformation, whereas the latter has a double dual-pistonmechanism for ... gradientformation), and that designed for flow rates between 0.05and 5 ml/min (high-pressure gradient formation).In separating the multiple constituents of a typical food sample, HPLC column ... used in food analysis. Conductivity detectors, on the other hand, arewell-suited for the sensitive and selective analysis of cationsand anions, and thermal energy detectors are used for high-sensitivity...

HPLC for Food Analysis phần 9 ppsx

... they are used prima-rily in the analysis of carbohydrates and nonaromatic acids.104<- [M + NH4]+Abundance10000080000 60 0002000040000 60 3 63 9987400 60 01000800m/zFigure 74Mass ... provide for automated drawing of the baselinesduring postrun replotting and for the plotting of calibrationcurves showing detector response. For unattended analysesin which several runs are performed ... arewell-suited for the modification of calibration tables and for the reanalysis of integration events and data. The softwaremust record such recalculation procedures so that the analysis can...

HPLC for Food Analysis phần 10 docx

... 28methylamine, 48meticlorpindol, 16 metronidazol, 16 microbial growth, 6 microorganism, 6 microsampling, 70milk, 16, 21, 22mixing noise, 81molecular weight, 66 monochromator, 89morpholine, ... spectral analysis , Agilent Primer 5 968 -9346E, 2000. 16. R. Schuster, “A comparison of pre-and post-column sample treatment for the analysis of glyphosate”,Agilent Application Note5091- 362 1E, ... differ-ent days. Reproducibility applies to an analysis performedin more than one laboratory. Any HPLC method used in food analysis should be tested for both repeatability andreproducibility.The...

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