Atomic Force Microscopy in Cell Biology Episode 2 Part 10 doc

... (1998). Stretching, tearing, and dissecting single molecules of DNA. Angew. Chem. Int. Ed. 37(16), 21 98 22 00, courtesy of Wiley–VCH. to any dissection and it maintained its shape during the entire ... Supercoiling, which originates from a topological constriction (generally from the underwinding of the two DNA chains), translates into geometric effects. The twisting and writhing of the cha...

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... 1 32, 24 3 25 2. 20 . Marsh, D. J., Jensen, P. K., and Spring, K. R. (1985) Computer-based determina- tion of size and shape in living cells. J. Microsc. 137, 28 1 29 2. 21 . Timbs, M. M. and Spring, ... cells in phosphate-buffered saline (PBS) 1 mM CaCl 2 , 3 mM KCl, 1 mM K 2 HPO 4 ; 2 mM MgCl 2 , 140 mM NaCl, 8 mM Na 2 HPO 4 ; pH 7.4 (2 × 5 min). 2. Depending upon the ep...

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... than 20 0 nL/min, resulting in volume changes inside the fluid cell of less than ±6 μL in 1 /2 h. A. Principle of Operation Two syringe pumps inject fluid into the AFM fluid cell in an alternating ... motion of laminin-1 as observed by atomic force microscopy. Biochemistry 37, 826 2– 826 7. Chen, C. H., and Hansma, H. G. (20 00). Basement Membrane Macromolecules: Insights from...

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... The AFM Imaging of Living Cells 21 5 2. Henderson, E., Haydon, P. G., and Sakaguchi, D. S. (19 92) Actin filament dy- namics in living glial cells imaged by atomic force microscopy. Science 25 7, 944– 1946. 3. ... Methods in Molecular Biology, vol. 24 2: Atomic Force Microscopy: Biomedical Methods and Applications Edited by: P. C. Braga and D. Ricci © Humana Press Inc., To...

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... decorated microtubulesusing tapping mode atomic force microscopy in fluids. Eur. Biophys. J. 28 , 611– 620 . 12. Lyubchenko, Yu., L., Shlyakhtenko, L. S., Harrington, R. E., Oden, P. I., and Lindsay, S. M. (1993) Atomic ... with atomic force microscopy operating in different modes. Ultramicroscopy 68, 121 – 128 . 15. Vater, W., Fritzsche, W., Schaper, A., Bohm, K. J., Unge...

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... sectioning using quantitative differential interference contrast light microscopy (20 ,21 ). Here, we present an alternative new method using atomic force microscopy (AFM) for measuring cell volume in ... in 10 mL of ice-cold fixation mix. 5. Spin down at 325 g for 10 min and remove supernantant, and resuspend pellet in 10 mL of ice-cold fixative, repeating twice. 6. Spi...

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... Ultramicroscopy 82, 28 9 29 5. Lehenkari, P. P., and Horton, M. A. (1999). Single integrin molecule adhesion forces in intact cells measured by atomic force microscopy. Biochem. Biophys. Res. Commun. 25 9, ... (1986). Atomic force microscope. Phys. Rev. Lett. 56, 930. Charras, G. T., and Horton, M. A. (20 02 A). Determination of cellular strains by combined atomic force mic...

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... Cells for live imaging are washed again in PBS containing 10 mg/ml FSG. The scanning experiments are carried out in culture medium for living cells and in PBS for fixed cells. In both cases, 10 ... reserved. 0091-679X/ 02 $35.00 21 3 22 2 Kindt et al. Fig. 5 Enzymatic degradation of single DNA molecules in the AFM. A field of DNA molecules (0.5 μg/ mL of BlueScript plasmid DNA)...

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... by atomic force microscopy. FEBS Lett. 381, 161–164. Mou, J., Sheng, S., Ho, R., and Shao, Z. (1996). Chaperonins GroEL and GroES: views form atomic force microscopy. Biophys. J. 71, 22 13 22 21. Mou, ... microtiter wells by scanning force microscopy. Langmuir 11, 1 822 –1 826 . Rogers, W., and Glaser, M. (1993). Distributions of proteins and lipids in erythrocyte membrane. Bi...

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... native 13. Atomic Force Microscopy and Spectroscopy of Membrane Proteins 27 1 Fig. 8 Contact mode AFM topograph of α-hemolysin oligomers. The α-hemolysin inserted into the phos- pholipid bilayer in 10 ... crystal. 13. Atomic Force Microscopy and Spectroscopy of Membrane Proteins 27 9 Fig. 14 Native bacteriorhodopsin assembled into an orthorhombic lattice. (A) In this cryst...

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