Atomic Force Microscopy in Cell Biology Episode 2 Part 7 pps

... = k B T L p  1 4(1 − x/L c ) 2 − 1 4 + x L c + i 7  i =2 a i  x L c  i  , with a 2 =−0.516 422 8, a 3 = 2. 73 7418, a 4 = 16. 074 97, a 5 =−38. 876 07, a 6 = 39.499944, and a 7 =−14. 177 18. The application ... by atomic force microscopy. Proc. Natl. Acad. Sci. U.S.A. 95, 12, 283– 12, 288. Gad, M., Itoh, A., and Ikai, A. (19 97) . Mapping cell wall polysaccharides of liv...

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... The AFM Imaging of Living Cells 21 5 2. Henderson, E., Haydon, P. G., and Sakaguchi, D. S. (19 92) Actin filament dy- namics in living glial cells imaged by atomic force microscopy. Science 25 7, 944– 1946. 3. ... adsorption the AFM of Protein Complexes 21 7 21 7 16 Atomic Force Microscopy of Protein Complexes Olga I. Kiselyova and Igor V. Yaminsky 1. Introduction Scann...

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... sectioning using quantitative differential interference contrast light microscopy (20 ,21 ). Here, we present an alternative new method using atomic force microscopy (AFM) for measuring cell volume in ... 24 8 Thalhammer et al. 2. Spin down at 325 g for 10 min. Discharge supernantant and resuspend pellet in 10 mL of hypotonic solution containing 0. 075 M KCl. Incubate at 37 C...

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... microtubules and kinesin decorated microtubulesusing tapping mode atomic force microscopy in fluids. Eur. Biophys. J. 28 , 611– 620 . 12. Lyubchenko, Yu., L., Shlyakhtenko, L. S., Harrington, R. E., ... with atomic force microscopy operating in different modes. Ultramicroscopy 68, 121 – 128 . 15. Vater, W., Fritzsche, W., Schaper, A., Bohm, K. J., Unger, E., and Jovin, T. M....

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... 1 32, 24 3 25 2. 20 . Marsh, D. J., Jensen, P. K., and Spring, K. R. (1985) Computer-based determina- tion of size and shape in living cells. J. Microsc. 1 37, 28 1 29 2. 21 . Timbs, M. M. and Spring, ... 69, 26 4 27 0. Aldosterone-Sensitive Cells Imaged With AFM 27 7 muscle and endothelial cells: subcellular localization of calcium elevations by single cell imaging. Biochem. Bio...

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... Ultramicroscopy 82, 28 9 29 5. Lehenkari, P. P., and Horton, M. A. (1999). Single integrin molecule adhesion forces in intact cells measured by atomic force microscopy. Biochem. Biophys. Res. Commun. 25 9, ... and Yu, L. (20 00). Atomic force microscopy imaging of living cells: A preliminary study of the disruptive effect of the cantilever tip on cell morphology. Ultramicrosc...

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... aldehyde groups. Cells for live imaging are washed again in PBS containing 10 mg/ml FSG. The scanning experiments are carried out in culture medium for living cells and in PBS for fixed cells. In both ... Plate.) Fig. 3 AFM imaging of laminin molecules in air shows submolecular structure in the laminin arms (top row). In the sequential images, a single laminin molecule in aqueo...

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... than 20 0 nL/min, resulting in volume changes inside the fluid cell of less than ±6 μL in 1 /2 h. A. Principle of Operation Two syringe pumps inject fluid into the AFM fluid cell in an alternating ... laminin-1 as observed by atomic force microscopy. Biochemistry 37, 826 2– 826 7. Chen, C. H., and Hansma, H. G. (20 00). Basement Membrane Macromolecules: Insights from Atomic...

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... by atomic force microscopy. FEBS Lett. 381, 161–164. Mou, J., Sheng, S., Ho, R., and Shao, Z. (1996). Chaperonins GroEL and GroES: views form atomic force microscopy. Biophys. J. 71 , 22 13 22 21. Mou, ... Biophys. J. 72 , 1308–1318. Zhang, Y., Sheng, S., and Shao, Z. (1996). Imaging biological structures with the cryo atomic force microscope. Biophys. J. 71 , 21 68 21 76 ....

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... crystal. 13. Atomic Force Microscopy and Spectroscopy of Membrane Proteins 27 9 Fig. 14 Native bacteriorhodopsin assembled into an orthorhombic lattice. (A) In this crystal form (p 22 1 2 1 ) the ... native 13. Atomic Force Microscopy and Spectroscopy of Membrane Proteins 27 1 Fig. 8 Contact mode AFM topograph of α-hemolysin oligomers. The α-hemolysin inserted into the phos...

Ngày tải lên: 06/08/2014, 02:20