Atomic Force Microscopy in Cell Biology Episode 2 Part 4 pdf

... by atomic force microscopy. FEBS Lett. 381, 161–1 64. Mou, J., Sheng, S., Ho, R., and Shao, Z. (1996). Chaperonins GroEL and GroES: views form atomic force microscopy. Biophys. J. 71, 22 13 22 21. Mou, ... 105–111. Perutz, M. F. (19 92) . Protein function below 22 0K. Nature 358, 548 . 12. Cryo-AFM 24 9 Fig. 5 Representative cryo-AFM images of single molecules: (a) C1q, (b) IgM...

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... slips bearing transfected cells in phosphate-buffered saline (PBS) 1 mM CaCl 2 , 3 mM KCl, 1 mM K 2 HPO 4 ; 2 mM MgCl 2 , 140 mM NaCl, 8 mM Na 2 HPO 4 ; pH 7 .4 (2 × 5 min). 2. Depending upon the ... 1 32, 24 3 25 2. 20 . Marsh, D. J., Jensen, P. K., and Spring, K. R. (1985) Computer-based determina- tion of size and shape in living cells. J. Microsc. 137, 28 1 29 2....

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... of the instrument permitting). In the future, we expect an increasing contribution by forced unfolding measurements to the understanding of protein folding as, on the one hand, proteins can be engineered ... of stiff chains. J. Chem. Phys. 56, 15 64 1568. Florin, E L., Moy, V. T., and Gaub, H. E. (19 94) . Adhesive forces between individual ligand receptor pairs. Science 26 4, 41 5 41 7....

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... Imaging of Living Cells 21 5 2. Henderson, E., Haydon, P. G., and Sakaguchi, D. S. (19 92) Actin filament dy- namics in living glial cells imaged by atomic force microscopy. Science 25 7, 944 – 1 946 . 3. ... adsorbed proteins. (A) Fp; (B) cytochrome P450; (C) mixture of Fp and cytochrome P450; (D) mixture of noninteracting proteins. AFM of Protein Complexes 22 1 3 .4. Image Interp...

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... J. 74, 29 83 29 95. 7. Von Nahmen, A., Schenk, M., Seiber, M., and Amrein, M. (1997) The structure of model surfactant as revealed by scanning force microscopy. Biophys. J. 72, 46 3 46 9. 23 4 Nag ... scanning tunneling microscopy. Nature 3 24 , 20 4 20 6. 8. Bustamante, C., Vesenka, J., Tang, C. L., Lees, W., Guthhold, M., and Keller, R. (19 92) Circular DNA molecules imaged in...

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... Atomic force microscope featuring an inte- grated optical microscope. Ultramicroscopy 42 / 44 , 1 549 –15 52. 14. Musio, A., Mariani, T., Frediani, C., Ascoli, C., and Sbrana, I. (1997) Atomic force ... sectioning using quantitative differential interference contrast light microscopy (20 ,21 ). Here, we present an alternative new method using atomic force microscopy (AFM) f...

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... Ultramicroscopy 82, 28 9 29 5. Lehenkari, P. P., and Horton, M. A. (1999). Single integrin molecule adhesion forces in intact cells measured by atomic force microscopy. Biochem. Biophys. Res. Commun. 25 9, 645 –650. Lundberg, ... using an atomic force microscope. J. Histochem. Cytochem. 48 , 719– 7 24 . Henderson, E., Haydon, P. G., and Sakaguchi, D. S. (19 92) . Actin filament d...

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... organelle transport measured in vivo by an infrared laser trap. Nature 42 8 , 346 – 348 . Cherry, R. J. (19 92) . Keeping track of cell surface receptors. Trends Cell Biol. 2, 24 2 24 4. Dabros, T., Warszynski, ... aldehyde groups. Cells for live imaging are washed again in PBS containing 10 mg/ml FSG. The scanning experiments are carried out in culture medium for living cells...

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... than 20 0 nL/min, resulting in volume changes inside the fluid cell of less than ±6 μL in 1 /2 h. A. Principle of Operation Two syringe pumps inject fluid into the AFM fluid cell in an alternating ... observed by atomic force microscopy. Biochemistry 37, 826 2– 826 7. Chen, C. H., and Hansma, H. G. (20 00). Basement Membrane Macromolecules: Insights from Atomic Force Micros...

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... crystal. 13. Atomic Force Microscopy and Spectroscopy of Membrane Proteins 27 9 Fig. 14 Native bacteriorhodopsin assembled into an orthorhombic lattice. (A) In this crystal form (p 22 1 2 1 ) the ... native 13. Atomic Force Microscopy and Spectroscopy of Membrane Proteins 27 1 Fig. 8 Contact mode AFM topograph of α-hemolysin oligomers. The α-hemolysin inserted into the pho...

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