Atomic Force Microscopy in Cell Biology Episode 1 Part 5 docx

... filaments, or determine the architecture of the network. Prominent examples of actin-binding pro- teins are α-actinin (bundler), gelsolin (severs filaments, binds to monomers), filamin (cross-links filaments), ... reserved. 00 91- 679X/02 $ 35. 00 67 4. Measuring Elastic Properties of Living Cells 85 Fig. 11 (continued ). and therefore it is capable of following biological processes in situ....

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... High 12 8. 410 kHz Low 12 5. 410 kHz Gain 1. 000 Vib. Voltage 1. 51 1 V LPF 1. 0 kHz HPF 1. 0 kHz Time 5 s Vib. Freq. 12 6.800 Amplitude 1. 068 V Peak Freq. 12 6. 918 kHz ∆F 0.3 35 Q 379.273 Phase 56 .52 0° h. ... W. (19 96) Scanning force microscopy. Cosmet. Toilet .11 1, 57 – 65. 5. You, H. and Yu, L. (19 97) Atomic force microscopy as a tool for study of human hair...

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... and Hansma, P. K. (19 90) Imaging cells with the atomic force microscope. J. Struct. Biol. 10 5, 54 – 61. 18 . Kasas, S. and Ikai, A. (19 96) A method for anchoring round shaped cells for atomic force microscope ... Henderson, E. R. (19 97) Cell viability and probe -cell mem- brane interactions of XR1 glial cells imaged by atomic force microscopy. Biophys. J. 73, 12 05 12...

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... dynamics in living glial cells imaged by atomic force microscopy. Science 257 , 19 44 19 46. Hoh, J. H., and Hansma, P. K. (19 92). Atomic force microscopy for high-resolution imaging in cell biology. Trends ... E., and Binnig, G. (19 92). In situ investigation of single living cell infected by viruses. Ultramicroscopy 42–44, 11 61 11 67. Henderson, E., Haydon, P. G.,...

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... Instrum. 71, 4 31 436. 15 . Radmacher, M., Cleveland, J. P., and Hansma, P. K. (19 95) Improvement of ther- mally induced bending of cantilevers used for atomic force microscopy. Scanning 17 , 11 7 12 1. 6 ... C. F. (19 89) Forces in atomic force microscopy in air and water. Appl. Phys. Lett. 54 , 26 51 2 653 . 4. Butt, H J., Siedle, P., Seifert, K., et al. (19 93) Scan s...

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... Microsc. 200, 10 9 11 3. 15 . Barrett, R. C. and Quate, C. F. (19 91) Optical scan-correction system applied to atomic force microscopy. Rev. Sci. Instrum. 62, 13 93 13 99. Artifacts in AFM 35 generate ... Van Haesendonck, C. (19 91) Can atomic force microscopy tips be inspected by atomic force microscopy? J. Vac. Sci. Technol. B. 9, 13 09 13 12. 3. Keller, D. and Chou...

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... skin fibroblasts and 3T3 cells are generally maintained as monolayer cultures in Dulbecco’s modified Eagles’ medium/Ham F12 con- taining 15 mM NaHCO 3 , 50 U/mL penicillin, 50 mg/mL streptomycin, ... needs to be tracked continuously. 3.2. AFM Imaging and F-d Analysis 3.2 .1. Imaging of Cells 3.2 .1. 1. FIXED OR DEHYDRATED CELLS When a cell is fixed, through cross-linking of the plasma...

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... filament dynamics in living glial cells imaged by atomic force microscopy. Science 257 , 19 44 19 46. 3. Hoh, J. H. and Hansma, P. K. (19 92) Atomic force microscopy for high-resolu- tion imaging in cell biology. ... by atomic force microscopy in the imaging and F-d modes. Int. J. Cosm. Sci. 23 ,16 5 17 4. 19 . Wu, H. W., Kuhn, T., and Moy, V. T. (19 98) Mechanica...

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... Swift, J. A. (19 91) Fine details on the surface of human hair. Int. J. Cosmet. Sci. 13 , 14 3 15 9. 17 . Smith, J. R. (19 97) Use of atomic force microscopy for high-resolution non-inva- sive structural ... cells: imaging and force- versus-distance investigations. J. Microscopy 17 6, 254 –2 61. 2. Oberleithner, H., Brinckmann, E., Giebisch, G., and Geibel, J. (19 95) Visualizi...

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... 313 31- 028). 9. Fetal bovine serum (FBS; heat inactivated; Gibco 10 108 - 15 7). 10 . Horse serum (heat inactivated; Gibco 26 050 -070). 11 . Poly-D-Lysine (Sigma P-7280) or Poly-L-Lysine (Sigma P- 9 15 5) . 12 . ... by atomic force microscopy. J. Cell Sci. 10 7, 11 05 11 14. 4. Ricci, D., Tedesco, M., and Grattarola, M. (19 97) Mechanical and morphological properties of living...

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