HPLC A Praactical User''''''''S Guide Part 8 ppt

HPLC A Praactical User''''S Guide Part 8 ppt

HPLC A Praactical User''''S Guide Part 8 ppt

... necessary, we can increase to the next size larger loop without affecting resolution. 1 38 PREPARATIVE CHROMATOGRAPHY Table 11.1 A Guide to Preparative Scale-up Analytical Semipreparative Preparative Size ... important. Here we are gathering grams of material for biological testing and structural analysis, and as reaction intermediates. HPLC: A Practical User’s Guide, Second Edition,...

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HPLC A Praactical User''''S Guide Part 1 ppt

HPLC A Praactical User''''S Guide Part 1 ppt

... is easier, faster, and cheaper to change the character of the solvent. 8 ADVANTAGES AND DISADVANTAGES OF HPLC CONTENTS PREFACE xi I HPLC PRIMER 1 1 Advantages and Disadvantages of HPLC 3 1.1 How ... recover the separated materi- als (preparative mode). It is very important to remember that HPLC is both an analytical and a preparative tool. Often the preparative capabilities of the...

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HPLC A Praactical User''''S Guide Part 2 doc

HPLC A Praactical User''''S Guide Part 2 doc

... of a packing material, sample clean-ups, and broad separations of classes of materials. They are available in almost any type of packing available for HPLC separations: partition, ion exchange, ... Service and support have always been the differentiating factors that really separate and define the best companies. 2.1.4 Brand Names and Clones A single supplier working on an O.E.M. basis h...

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HPLC A Praactical User''''S Guide Part 3 doc

HPLC A Praactical User''''S Guide Part 3 doc

... peak height times the half peak width. The areas of each peak are summed to give a total area. Dividing this into the area of each peak gives a relative area percentage for each peak. Like peak ... 1 to 8 for analytical separations and 4 to 12 for preparative. a s range from 1 to 2; at a = 1, peaks completely overlap, much above a = 2 and the separation can be made in a separator...

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HPLC A Praactical User''''S Guide Part 4 pdf

HPLC A Praactical User''''S Guide Part 4 pdf

... use a technique called sequential analysis (Fig. 5.6). For example, we can make a size separation, then take a size fraction and do a partition separation. This is commonly used in separating a complex ... Anionic packing materials have an affinity for anions (negatively charged ions) and have positively charged functional groups on their surfaces, usually organic amines. Cationic pa...

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HPLC A Praactical User''''S Guide Part 5 pot

HPLC A Praactical User''''S Guide Part 5 pot

... form as a pair of peaks or a badly tailing peak for each compound. Ion-pairing reactions can also be carried out using quaternary amines as counter-charges for organic acid and organic phosphate ... I’m talking about a change that takes place gradually or all at once and remains high. Be aware that gradient mixtures of some solvents like methanol and water go through a pressure maximu...

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HPLC A Praactical User''''S Guide Part 6 potx

HPLC A Praactical User''''S Guide Part 6 potx

... covalently bonded phases available on zirconium that can simulate silica C 18 separations with a- type peak shifting, but bonded phases are available that provide truly unique sep- arations. Many ... monosaccharides and separate alcohol sugars from each other. 8. 1.2 Anionic: Weak and Strong In strong anionic ion exchangers, the quaternary amine is attached to the silica surface by an alk...

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HPLC A Praactical User''''S Guide Part 7 docx

HPLC A Praactical User''''S Guide Part 7 docx

... than the inte- grator to store a single chromatogram, however, it can use this raw data to report tables of peak maxima, areas, and heights versus time and recalculate, redisplay, and compare ... reports each as a percent of the total. The computer handles data differently. It measures the voltage at a preset time interval and stores each displacement as a digital word, usually at a...

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HPLC A Praactical User''''S Guide Part 9 docx

HPLC A Praactical User''''S Guide Part 9 docx

... concentrations of the standards are injected, areas are measured, and a calibration curve is platted. Unknown samples are then injected, chromatograms run, and areas are calculated and compared ... response factors are calculated relative to the internal standards, and amounts of each unknown present are calculated from the standards calibration factors. Internal standards are usually used...

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HPLC A Praactical User''''S Guide Part 10 pot

HPLC A Praactical User''''S Guide Part 10 pot

... addresses, and macro programs. 14.6.3 Databases: .DB2 Format To export data files into a database program, a database file format called .DB2 was developed in an early PC database, dBase II. Databases ... computer and have been retained as a standard format. .WK files are Lotus-1,2,3 formats and .XLS are Microsoft Excel formats that have become spreadsheet standards, allowing transfer of d...

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