... Line 5, an expanded section of the parts of UL6, UL8 and whole of UL7 open reading flame. Line 6, a schematic diagram of the recombinant virus genome. As a result of RecET mutagenesis, a kanamycin-resistant ... indi-cated that the viral titer of MT102 (ΔUL7) was reducednearly 100-fold compared to that of wild-type YK304 at48 h post-infection. Even at an MOI of 3, the yield of MT102 (ΔUL7) at 24 h post-infection ... post-infection was about 10-foldless than that of wild-type YK304 (Figure 2A). The growthcurves of MT103 (repair) at MoI of 0.01 and 3 were almostthe same as those of parental virus YK304, indicating...