Warm-up Activity doc

Ngày tải lên: 05/09/2013, 23:10

Ngày tải lên: 06/09/2013, 01:10

... 30 Time (h) Activity (%) 0 2 4 0 5 10 15 20 25 30 Time (h) Ln % activity –2 0 4 8 12 16 20 70 80 90 100 110 120 Temperature (°C) Cp (kcal/mol °C) 130 Fig. 7. (A) Kinetic stability of McoP. The activity ... Winooski, VT, USA). Cu + oxidation activity was measured in terms of oxygen consumption rates by using an oxygraph, as previously described [5]. The optimal temperature for the act...

Ngày tải lên: 18/02/2014, 04:20

... in epimerase activity (Fig. 4B) while the consti- tutive mutarotase activity remained the same. More importantly, with the increase in epimerase activity, the coeluting mutarotase activity was ... epimerase activity also showed mutarotase activity. If S. cerevisiae epimerase were a truly bifunctional enzyme, then induction with galactose should induce both epimerase and mutarotase...

Ngày tải lên: 19/02/2014, 12:20

... Prm3 +786 8 01234567 0246 12810 0 5 10 15 20 25 Luciferase Activity (RLU) Luciferase Activity (RLU) Luciferase Activit y (RLU) Luciferase Activity (RLU) A CD B -1394 +1 Luc +1 Luc -975 pGL3Basic +1 Luc -50 +1 ... However, the level of )404 to +119 directed luciferase activity was not significantly different from that of the Prm3-directed luciferase activity (e.g. )404 to +1) express...

Ngày tải lên: 19/02/2014, 16:20

... partial activity could be recovered under reducing conditions, this activity remaining threefold lower than that of the wild-type. Figure 3 illustrates the effect of reduced MV + on H–D exchange activity ... than 15%. Proteins Aerobiosis Anaerobiosis Anaerobiosis + MV + Activity HD formation H 2 formation Activity HD formation H 2 formation Activity HD formation H 2 formation HupU...

Ngày tải lên: 20/02/2014, 01:20

... structure of MGD1, only the loop part seems to convey activity. Activity is directed mainly against Gram-positive bacteria and fungi The activity spectra of peptides B, K, M and Q were compared ... the loop 3 sequence and its bacteriostatic activity is in agreement with the general observation that the basicity of defensins is an important parameter for activity, in particular because...

Ngày tải lên: 20/02/2014, 11:20

... family and by modulating the intrinsic activity (or, degree of occlusion) of glucose transporters (Fig. 9). Fig. 5. The involvement of tyrosine kinase activity in arsenite-induced glucose uptake. ... Biochem. 270) Ó FEBS 2003 activity of this enzyme (in conjunction with other signals) to induce GLUT-4 translocation in 3T3-L1 adipocytes. Of note, the basal levels of PKC-k activity are...

Ngày tải lên: 21/02/2014, 00:20

... mutation resulted in the selenoenzyme Se-2F3- scFv. The GPX activity of Se-2F3-scFv was determined by the coupled coenzyme system. One unit of activity is defined as the amount of compound that utilizes ... 100 m M potassium phosphate buffer, pH 7.0, and an aliquot was taken for assay of CCO activity [35]. The CCO activity was measured in 2 mL of the reaction system, in which the cytoc...

Ngày tải lên: 21/02/2014, 00:20

... levels of luciferase activity than did L4.0. Among the deletion constructs tested, L1.2 had the highest relative luciferase activity. L0.5 also showed a comparable high activity. These results ... promoter activity. MKN1 cells were transfected with wild type (K3S2) or mutated constructs. Relative luciferase activity was determined in triplicate, and data were nor- malized to b-galactos...

Ngày tải lên: 21/02/2014, 03:20