... The forward p rimer 5¢-ATGACTATTTCTTCTGCACATCCAGAGACAGAAC-3¢ con tains the initiation codon,while the reverse primer 5¢-CTTTCTGCAGACTCATTCGCTGATATATATTC-3¢ linked a PstI restriction siteto the ... Okuda, S., Takesada, Y., I izuka, M.,Mimamiura, N. & Imanaka, T. (1992) Action of neopullulanasecatalyzes both hydrolysis and transglycosilation at a- (1-4)- and a- (1-6)-glucosidic linkages. J. ... a- glucosidaseMalL (sucras e-isomaltase-maltase) of Bacillus subtilis. J. Bacteriol.180, 2 574–2578.35. Nakao, M., N akayama, T., Kakudo, A. , Inohara, M., Harada,M., O mura, F. & Shibano,...