... addition of ATP, ANT and F 0 F 1 -ATP synthase operate in the reverse mode. Provision of exogenous ATP leads to ATP influx to mitochondria, followed by its hydrolysis by the reversed F 0 F 1 -ATP synthase, ... access to the F 0 F 1 -ATP synthase. In intact mitochondria, changes in ATP synthesis or hydrolysis rates by the F 0 F 1 -ATP synthase do not nec- essarily translat...
Ngày tải lên: 14/02/2014, 19:20
... bacterial ATP synthase raised the question whether the stoichiometry of V 0 :F 0 -like subunits may be flexible and thus a mechanism to change the action of the enzyme from ATP synthase to ATPase. ... V-type ATP synthases and ATPases: reversals in function and changes in the H + ⁄ ATP stoichiometry. FEBS Lett 576, 1–4. 13 Forgac M (1999) Structure and properties of the vacuo- lar H...
Ngày tải lên: 18/02/2014, 17:20
Tài liệu Báo cáo khoa học: A point mutation in the ATP synthase of Rhodobacter capsulatus results in differential contributions of DpH and Du in driving the ATP synthesis reaction pptx
... of DpH and Du in driving ATP synthesis. Keywords: ATP synthase; mutagenesis; Rhodobacter cap- sulatus; DpH; Du. Membrane-bound F 0 F 1 -ATPases (ATP synthases) catalyze ATP synthesis in bacteria, ... the ATP synthesis rate o f an ATP synthase differently according to whether a Du is present or not. To our knowledge, no previous reports of a similar effect exist in the literature...
Ngày tải lên: 21/02/2014, 03:20
Báo cáo khoa học: Expression of the Drosophila melanogaster ATP synthase a subunit gene is regulated by a transcriptional element containing GAF and Adf-1 binding sites pptx
... and functionally, with highly developed c ristae full of ATP synthase complexes. For this reason, ATP synthase and in particular the a-F1-ATPase and b-F1-ATPase catalytic subunits have been often used ... the d-aminolevulinate synthase (ALAS)andb-F1-ATPase genes [29,34]. In both constructs there was a s ubstantial Fig. 3. Functional analysis of the D. melanogaster a-F1-ATPase promot...
Ngày tải lên: 07/03/2014, 16:20
Báo cáo khoa học: Structural evidence for a constant c11 ring stoichiometry in the sodium F-ATP synthase doc
... sodium ATP synthases. J Mol Biol 325, 389–397. 21 Seelert H, Dencher NA & Mu ¨ ller DJ (2003) Fourteen protomers compose the oligomer III of the proton-rotor in spinach chloroplast ATP synthase. ... stability of the turbines of Na + -F 1 F o ATP synthases. EMBO Rep 3, 1094–1098. 23 Van der Laan M, Bechtluft P, Kol S, Nouwen N & Driessen AJ (2004) F 1 F 0 ATP synthase subuni...
Ngày tải lên: 07/03/2014, 21:20
Báo cáo Y học: Membrane embedded location of Na+ or H+ binding sites on the rotor ring of F1F0 ATP synthases ppt
... F 1 F 0 ATP synthases. Labelling of these sites with the fluorescent derivative N-PCD provides unique options to monitor by fluorescence Fig. 1. SDS/PAGE of purified E. coli ATP synthase. Purified ATP synthase ... of F 1 F 0 ATP synthase from I. tartaricus The F 1 F 0 ATP synthase was purified from whole cells of I. tartaricus by fractionated precipitation with polyethyl- eneglyco...
Ngày tải lên: 08/03/2014, 09:20
Báo cáo khoa học: NMR investigations of subunit c of the ATP synthase from Propionigenium modestum in chloroform/methanol/water (4 : 4 : 1) pot
... subunit c of E. coli. Keywords: ATP synthase; stable isotope labeling, NMR spectroscopy; Propionigenium modestum; subunit c. F 1 F 0 ATP synthases catalyse the formation of ATP from ADP and inorganic ... 1632 55 23, E-mail: matthey@micro.biol.ethz.ch Enzymes:H + -transporting ATP synthase (EC 3.6.1.34); Na + -transporting ATP synthase (EC 3.6.1.37). Note: web page available at...
Ngày tải lên: 08/03/2014, 10:20
Báo cáo khoa học: Arginine-induced conformational change in the c-ring ⁄a-subunit interface of ATP synthase ppt
... whole atp operon (atpIBEFHAGDC)ofI. tartaricus [24] with the following modifications: the start codons of atpF and atpA were changed from TTG to ATG, a Bsu15I single site was intro- duced between atpE ... indicate Time (s) 0 102030405060 mol ATP/ mol enzyme 0 200 400 600 800 wt cG25I cG68C cT67C Fig. 5. ATP synthase activities in the wild-type I. tartaricus ATP synthase and c subuni...
Ngày tải lên: 16/03/2014, 06:20
Báo cáo khoa học: Efficient ATP synthesis by thermophilic Bacillus FoF1-ATP synthase ppt
... and Dw-driven ATP synthesis catalyzed by the H + -ATPases from Escherichia coli or chloroplasts reconstituted into liposomes. FEBS Lett 457, 327–332. ATP synthesis by thermophilic ATP synthase N. ... ATP synthesis by thermophilic ATP synthase FEBS Journal 278 (2011) 2647–2654 ª 2011 The Authors Journal compilation ª 2011 FEBS 2649 Efficient ATP synthesis by thermophilic Bacillu...
Ngày tải lên: 22/03/2014, 16:20
Báo cáo khoa học: Pigment epithelium-derived factor binds to cell-surface F1-ATP synthase pdf
... endothelial cell-surface F 1 -ATPase ⁄ ATP synthase b-subunit, and inhibits endothelial extracellular ATP synthesis activity. The findings imply that F 1 F o -ATP synthase may act as a receptor ... F 1 -ATP synthase b-subunit (Table S1). Until recently, F 1 F o -ATP synthase expres- sion was assumed to be strictly confined to mitochon- dria, where it generates most of the cell...
Ngày tải lên: 22/03/2014, 21:21