... r a Þwhere rnand ruare the experimentally determinednumerical values of the ratio a/ b, and r a is the theoreticalnumerical value of ratio a/ b for a mixture of aromaticamino acids (Tyr and ... a value of 8.0, byadding, alternatively, pH-unadjusted stock solutions of Trisand EDTA. The final concentration of EDTA was 5 mM.Under these conditions, the pale blue of the supernatant at pH ... not mark any as pooror inappropriate. Another structural analysis, obtained by the VERIFY3Dprogram [61], gave an average value of 0.21,which is greater than zero, the quality value indicated...