... from the amino terminus, was amplified by PCR using the following primers: forward, 5¢-CATGCCATGGCGCCATTTTTCTTGAGACATGCC-3¢; reverse,5¢-CTGGGATCCGTCCGAATCAGGTTCCTTC-3¢(purchased from Sigma), and ... 5,6-dibromo- 1-( b-D-ribofuranosyl)benzimidazole; DRBT, 5,6-dichloro- 1-( b-D-ribofuranosyl)benzo-triazole; a-DMRB, 5,6-dimethyl- 1-( a-D-ribofuranosyl)benzimidazole;TCBT, 4,5,6,7,-tetrachlorobenzotriazole; ... unlike the case with casein kinase 2, the modeof action of the inhibitors vs the helicases is not by interaction with the catalytic ATP-binding site, but rather by occupation of an allosteric nucleoside/nucleotide...