... ¼ 200 nM).Steady-state data (kcat, Km and the arithmetically calcu-lated selectivity coefficient, kcat/Km) for oxidation of L-ascorbic acid and guaiacol by rAPX and H42E are shownin ... yeast cytochrome c peroxi-dase that strongly affects the kinetics of ferrocytochrome c oxidation. Biochemistry 27, 624 3– 6256 .7. Patterson, W.R. & Poulos, T.L. ( 1994 ) Characterization and crystallization ... and crystallization of recombinant pea cytosolic ascorbate peroxidase.J. Biol. Chem. 269, 1702 0–1 7024.8. Mittler, R. & Zilinskas, B.A. ( 1991 ) Purification and character-isation of pea cytosolic ascorbate...