Promoter pot

... the T7 promoter (the Ø10 promoter from Bacteriophage T7) is positioned in the E. coli genome and governed by the lacUV5 promoter [1]. Another important criterion when choosing a suit- able promoter, ... E. coli include the T7 promoter, which originates from bacteriophage T7 [1] and the E. coli lac promoter [2], as well as its modified form lacUV5 [3]. The synthetic trc promoter [4]...

Ngày tải lên: 06/03/2014, 01:20

... bp PR-1a promoter proved to be stronger and more reliable on average than expression from the 0.9 kb promoter (Fig. 3) [7]. On the other hand, gene expression from the )1533 bp PR-1a promoter, ... as found in the CaMV 35S RNA promoter (in bold letters). The as-1 element was inserted at position )591 in the PR-1a promoter. The as-1 containing )906 bp PR-1a promoter region was added a...

Ngày tải lên: 23/03/2014, 15:21

... significant effects on the promoter: the 5¢- and 3¢-intron modules increased promoter activity with 127 AU and 47 AU, respectively, whereas the middle intron module decreased promoter activity with ... positioned in close proximity to the promoter as the consequence of a distance effect (see [25,26]): apparently, the upstream enhancer has difficulty contacting the promoter when the 178...

Ngày tải lên: 23/03/2014, 20:22

... (AP-2a), in the promoter region of KCTD10. We found that the proximal promoter region from )108 to +30 was indispensable for basal promoter activity of KCTD10; and SP1 and AP-2a can regulate the promoter ... KCTD10 promoter structures, cloning of the promoter fragment, construction of deletion and point-mutated vectors Promoter searching was performed using proscan (http:// www-...

Ngày tải lên: 30/03/2014, 02:20

... genome. Baculovirus containing the human Flt-1 promoter drives endothelial-specific gene expression in vitro We generated a novel recombinant baculovirus with the human flt-1 promoter driving the expression ... baculovirus containing a 761-bp DNA fragment of the cytomegalovirus (CMV) promoter driving the expression of GFP (Figure 1a). This promoter was selected because it drives high...

Ngày tải lên: 18/06/2014, 18:20

... GCV OD-595 0.0 0.1 0.2 0.3 0.4 0.5 Mock Ad5-EGFP Ad5-TK Ad5- TK/EGFP C Ad5-E6mut Mock E6mut (18 kDa , Anti-Flag) D CMV promoter SwaI BstBI ClaI Amp R PacI PacI ITR ITR pAd5CMV/TCS Anti-TK EGFP Hoechst Mock Ad5-EGFP Ad5-TK Ad5-TK/EGFP Anti-E6 Anti-Flag Ad5- E6mut Mock Hoechst SwaI BstBI ClaI ... Access Short report A novel adenovirus vector for easy cloning in the E3 region downstream of t...

Ngày tải lên: 20/06/2014, 01:20

... kết giữa promoter (P) đối với phức hệ nhân tố sigma - [RNAP]] (R) là một quá trình gồm 2 bước: 1. R+P ↔ RP(đóng). K = 10E7 2. RP(đóng) > RP(mở). K = 10E-2 Promoter Promoter ... thiết yếu cho phép một gene có thể tiến hành phiên mã tạo mRNA. Các thành phần của Promoter  Phần lõi (Core) promoter  Vị trí khởi đầu phiên mã (TSS)  vị trí khoảng -35  Vị tr...

Ngày tải lên: 06/07/2014, 03:20

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Ngày tải lên: 08/03/2014, 01:20

... the promoter variant pD7egrGFL by a factor of 1.4 (p < 0 .05). Similar data of induction of the Egr-1 promoter by ionizing radiation h ave been reported recently [29]. Using synthetic promoter ... of t he wild-type Egr-1 promoter construct was roughly 10±12% of the activity displayed b y a control RSV promoter. As demonstrated in Fig. 2, the relative activity of promoter varian...

Ngày tải lên: 17/03/2014, 17:20

... the lipoprotein lipase promoter. J. Biol. Chem. 275, 11404–11411. Ó FEBS 2002 Rat Bsep promoter analysis (Eur. J. Biochem. 269) 3503 therefore designated the minimal Bsep promoter, appar- ently ... box of transcription initiation could be found within the minimal Bsep promoter. Additional consensus motifs of the minimal promoter include three AP1 sites and a CCAAT box. As opposed to th...

Ngày tải lên: 17/03/2014, 23:20