... same(Fig. 5G, lanes 5 and 6). Nevertheless, we sometimesobserved a decrease in the intensity of the expectedbands and appearance of a large smear, even in anunboiled sample of cells transfected with ... Crystal structure analysis revealed that theresidues Pro 42 , Lys 46 , Arg 15 4 , Ser 15 7, Ser 17 8and Lys 17 9are located close to each other on the surface of themouse Shh-N protein and are essential ... N-terminalpalmitoylation of ShhMammalian Gup1 has been described in the gene data-base cited above as a homolog of the S. cerevisiae GUP1gene product, based on its sequence homology. Align-ment of mouse and...