Introduction to Modern Liquid Chromatography, Third Edition part 60 pot
Introduction to Modern Liquid Chromatography, Third Edition part 60 pot
... the transfer, and to carry out the method so as to identify any potential issues that may need to be resolved prior to finalization of the transfer protocol. The sending laboratory should provide ... concentrations might be provided to both the sending and receiving laboratories; the individual laboratory results would then be compared to the true values to qualify the receiving...
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Introduction to Modern Liquid Chromatography, Third Edition part 2 potx
... 158 4.3 Introduction to Individual Detectors, 160 4.4 UV-Visible Detectors, 160 4.4.1 Fixed-Wavelength Detectors, 163 4.4.2 Variable-Wavelength Detectors, 164 4.4.3 Diode-Array Detectors, 165 4.4.4 ... 113 3.6 Autosamplers, 113 3.6.1 Six-Port Injection Valves, 114 3.6.1.1 Filled-Loop Injection, 114 3.6.1.2 Partial-Loop Injection, 115 3.6.2 Autosampler Designs, 116 3.6.2.1 Pull -to- Fill...
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Introduction to Modern Liquid Chromatography, Third Edition part 12 potx
... reasons: • to avoid an undesirable change in separation due to a sample size that is too large • to increase detection sensitivity for trace analysis, by using the largest possible sample size • to maximize ... 1 to 4, the peak begins to widen and then develops a flat top. For Equation (2.27) and the examples of Figure 2.22, we assume the delivery of an undistorted (i.e., cylindric...
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Introduction to Modern Liquid Chromatography, Third Edition part 15 pot
... the total of the autosampler -to- column plus column -to- detector connections. Sometimes it is advantageous to use a short piece of 0.007-in i.d. tubing between the autosampler and column, so as to ... with pressures up to 600 0 psi between the pump and detector, so the tubing must be able to withstand such pressures. Also tubing used to transport the sample from the autosampler...
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Introduction to Modern Liquid Chromatography, Third Edition part 22 potx
... directed to a photodetector, where it is monitored and converted to an electronic signal for data processing. Because fluorescence is emitted in all directions, it is common to monitor the emitted ... 240 250 260 270 280 Wavelength (nm) Absorbance (b) X Y X ( 260 nm) Y ( 260 nm) X + Y ( 260 nm) (a) Figure 4.12 Illustration of spectral deconvolution of analytes. (a) Hypothetical chro-...
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Introduction to Modern Liquid Chromatography, Third Edition part 29 potx
... pack- ing particles. To prevent particle aggregation, the interaction of the particle with the slurry liquid should be stronger than interactions between particles. For example, C 8 -orC 18 -modified particles ... as illustrated by the cartoon of Figure 5.25a, the liquid may be acceptable. However, if the particles tend to aggregate as in Figure 5.25b, the liquid is likely a poor cho...
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Introduction to Modern Liquid Chromatography, Third Edition part 30 pot
... REVERSED-PHASE CHROMATOGRAPHY (NARP), 295 6.6 SPECIAL PROBLEMS, 297 6.6.1 Poor Retention of Very Polar Samples, 297 6.6.2 Peak Tailing, 298 Introduction to Modern Liquid Chromatography, Third Edition, by ... and J. J. Kirkland, Introduction to Modern Liquid Chromatography, 2nd ed., Wiley, New York, 1979, ch. 5. 78. H. A. Claessens, M. A. Van Straten, and J. J. Kirkland,...
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Introduction to Modern Liquid Chromatography, Third Edition part 42 pot
... so that on average a change in %B by a factor of two (e.g., a change in mobile phase from 100% B to 50% B) will change values of k by a factor of 2 to 4. Similar plots of log k against log %B ... Wavelength (nm) 200 210 220 230 240 250 260 Acetonitrile a,b 0.52 3.1 0.03 0.02 0.00 0.00 0.00 0.00 0.00 Ethoxynonafluorobutane 0.01 h Presumed similar to ethyl ether Chloroform c 0.26 5.0 >...
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Introduction to Modern Liquid Chromatography, Third Edition part 53 pot
... gradient time t G (20, 60 min) and temperature T (30, 60 ◦ C), in order to simultaneously optimize %B and temperature T. The following experimental data are entered into the computer (see Fig. ... column. Because of the increase in pressure due to the smaller particles, it is necessary to reduce the flow rate from 2.0 mL/min in Figure 10.3b to 1.0 mL/min in the separa- tion of Figu...
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Introduction to Modern Liquid Chromatography, Third Edition part 55 pot
... hand. Second, the data system and associated software must be able to convert the HPLC Introduction to Modern Liquid Chromatography, Third Edition, by Lloyd R. Snyder, Joseph J. Kirkland, and John W. ... quantification. One way to minimize this problem is to stop integration when the peak is sure to have left the detector. This can be accomplished by visually determining w...
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