Introduction to Modern Liquid Chromatography, Third Edition part 49 ppsx
Introduction to Modern Liquid Chromatography, Third Edition part 49 ppsx
... Section 9.2.2.5: (1) to clean the column between
sample injections, (2) to shorten run time, or (3) to improve separation by adjusting
selectivity for different parts of the chromatogram. Because ... that the sample may be too nonpolar for separation with the usual
RPC conditions. In such cases an acetonitrile/buffer gradient can be replaced by
a gradient from acetonitrile to a less-p...
Introduction to Modern Liquid Chromatography, Third Edition part 3 ppsx
... 651
13.10.3 Liquid- Chromatography Modes for Polymer
Analysis, 653
13.10.3.1 Size-Exclusion
Chromatography, 653
13.10.3.2 Interactive Liquid
Chromatography, 653
13.10.3.3 Liquid Chromatography under
Critical ... RPC-
5
Chromatography, 623
13.5.3 Hydrophobic Interaction
Chromatography, 624
13.6 Separation of Carbohydrates, 625
13.6.1 Hydrophilic Interaction
Chromatography, 625
1...
Introduction to Modern Liquid Chromatography, Third Edition part 13 ppsx
... Snyder and J. J. Kirkland, Introduction to Modern Liquid Chromatography, 2nd
ed., Wiley-Interscience, New York, 1979, pp. 34–36.
46. Reviewer Guidance. Validation of Chromatographic Methods, Center ... Chromatogr. A, 1119 (2006) 140.
22. J. C. Giddings, Dynamics of Chromatography, Part 1, Principles and Theory, Dekker,
New York, 1965.
23. G. Guiochon, in High-Performance Liquid...
Introduction to Modern Liquid Chromatography, Third Edition part 34 ppsx
... in flow rate
from 2.0 to 1.0 mL/min (not shown) results in an insignificant increase in resolution
(to R
s
= 1.2), while run time is doubled to 10 min. Alternatively, the particle size
can be reduced, ... (called
‘‘phase-optimized liquid chromatography’’ or POPLC
®
from Bischoff). Experiments
with individual columns define sample retention for each column type, and it is then
possible t...
Introduction to Modern Liquid Chromatography, Third Edition part 63 ppsx
... proportional to
the accessible surface area of the packing (Section 5.2.1). For smaller molecules
(<1000 Da), particles with pore-diameters of 8 to 12 nm permit free access of
solutes into the pore ... surface of the particle (which represents < 1% of the total surface and
column capacity within the pores). In order to achieve adequate column capacity,
retention, and column efficie...
Introduction to Modern Liquid Chromatography, Third Edition part 66 ppsx
... elution strategy is to bind proteins at
pH values between 7 and 8, followed by elution with a step or gradient to pH values
between 4 and 5, in order to convert histidine residues to the ionized form.
An ... silanols.
Poly(2-hydroxyethyl aspartamide) is a stationary phase that was designed expressly
for HILIC [77]; it is prepared by incorporating ethanolamine into a coating of
polysuc...
Introduction to Modern Liquid Chromatography, Third Edition part 75 ppsx
... found to be powerful chiral selectors with complementary
enantioselectivity (different enantioselectivities and elution orders), leading to CSPs
based on vancomycin [111], teicoplanin [112], ristocetin ... antibiotic columns,
exemplified by enantiomer separations of Z-Ala (top) and 5-methyl-5-phenylhydantoin
(bottom). Experimental conditions: Ristocetin A, 20:80 methanol/0.1% triethylammon...
Introduction to Modern Liquid Chromatography, Third Edition part 84 ppsx
... as
acetonitrile or acetone to a homogenized portion of a vegetable sample allows the
extraction of pesticides into the solvent (step 2 of Fig. 16.7). Subsequent addition
of salt (step 3) leads to ... extractors and
thimbles are available for mg-size samples. The analyte concentration, the necessary
mass to obtain a representative sample, and the chromatographic detector sensitivity,
tog...
Introduction to Modern Liquid Chromatography, Third Edition part 87 ppsx
... screwdriver or other metal tool, to avoid damaging the pump head. Do
not use the piston to pry out the seal because the piston is likely to break. As
you remove the seal, be sure to note how the seal ... sapphire
piston.
• Inspect the piston for any scratches or defects. A simple way to highlight any
problems is to hold a laser pointer up to the end of the piston, which will
cau...
Introduction to Modern Liquid Chromatography, Third Edition part 88 ppsx
... (arrow).
counter to automatically record the number of injection cycles, so as to allow the
remaining rotor seal life to be estimated. When viewed in cross section, the grooves
of the rotor shown in ... split and distorted peaks in
Section 17.4.5.3); otherwise, it may be necessary to replace the column.
Before putting the system back into service, consider if steps need to be taken...
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