Introduction to Modern Liquid Chromatography, Third Edition part 47 pps

... reduced too much, can be compared with Figure 9.7d, where initial-%B is increased too much; in each case the resulting separation is unsatisfactory—either resolution is too low or run time is too ... different purposes: • to clean the column between sample injections • to shorten run time • to increase resolution by adjusting selectivity for different parts of the chromatogram (for ‘‘ir...

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... 651 13.10.3 Liquid- Chromatography Modes for Polymer Analysis, 653 13.10.3.1 Size-Exclusion Chromatography, 653 13.10.3.2 Interactive Liquid Chromatography, 653 13.10.3.3 Liquid Chromatography under Critical ... RPC- 5 Chromatography, 623 13.5.3 Hydrophobic Interaction Chromatography, 624 13.6 Separation of Carbohydrates, 625 13.6.1 Hydrophilic Interaction Chromatography, 625 1...

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... the early days of HPLC the technique was sometimes referred to as ‘‘high-pressure liquid chromatography’’ or ‘‘high-speed liquid chromatography, ’ for reasons suggested by Figure 1.5g. Figure 1.5h ... liquid liquid partition chromatography that was later improved in various ways. HPLC with a liquid stationary phase was since replaced by bonded-phase HPLC, the use of CCC as an alter...

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... Snyder and J. J. Kirkland, Introduction to Modern Liquid Chromatography, 2nd ed., Wiley-Interscience, New York, 1979, pp. 34–36. 46. Reviewer Guidance. Validation of Chromatographic Methods, Center ... Chromatogr. A, 1119 (2006) 140. 22. J. C. Giddings, Dynamics of Chromatography, Part 1, Principles and Theory, Dekker, New York, 1965. 23. G. Guiochon, in High-Performance Liquid...

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... 130 3.8.5 Report Generation, 130 3.8.6 Regulatory Functions, 130 3.9 EXTRA-COLUMN EFFECTS, 131 Introduction to Modern Liquid Chromatography, Third Edition, by Lloyd R. Snyder, Joseph J. Kirkland, ... the pump. The frit acts as a weight to keep the inlet tubing at the bottom of the reservoir, but its primary function is to provide backup filtration to remove particulate matte...

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... developed, it is likely to be used over an extended period and in different laboratories. Columns degrade during routine use and must therefore be replaced from time to time. As discussed in ... has lim- ited practical application. The reader may therefore wish to skip to following Section 6.3.6. 284 REVERSED-PHASE CHROMATOGRAPHY FOR NEUTRAL SAMPLES 0 10203040 Original (primary) metho...

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... in flow rate from 2.0 to 1.0 mL/min (not shown) results in an insignificant increase in resolution (to R s = 1.2), while run time is doubled to 10 min. Alternatively, the particle size can be reduced, ... (called ‘‘phase-optimized liquid chromatography’’ or POPLC ® from Bischoff). Experiments with individual columns define sample retention for each column type, and it is then possible t...

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... the analysis of any mixture that contained organic acids or bases. Together with liquid liquid partition and adsorption chromatography, IEC then accounted for most of the reported separations ... CHROMATOGRAPHY (IEC) Ion-exchange chromatography (IEC) is an important separation technique, but for the most part, today, with limited applications: • mixtures of inorganic ions (ion chromatogr...

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... chromatography (NPC). Another term used to describe NPC is adsorption chromatography,in recognition of the fact that retained solute molecules are attached to (or adsorbed onto) the surface of particles ... standards by anion-exchange chro- matography with amperometric detection. Sample: 1, myo-inositol; 2, D-sorbitol; 3, lactitol; 4, L-fructose; 5, rhamnose; 6, D-galactose; 7, D-glucosam...

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... affected by which isomer can better position itself adjacent to the surface, so as to allow each polar methoxy group in the molecule to interact with an adjacent silanol group on the surface. In ... silica columns—compared to polar-bonded-phase columns. Consequently a change from silica to a polar-bonded-phase column, with further variation of the mobile phase (change in %B and/or B...

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