Handbook of Microbiological Media, Fourth Edition part 166 ppt
Handbook of Microbiological Media, Fourth Edition part 166 ppt
... freshly.
Preparation of Medium: To 850.0mL of cooled, sterile solution A,
aseptically and anaerobically add in the following order: 1.0mL of ster-
ile solution B, 100.0mL of sterile solution C, 10.0mL of sterile ... 100.0mL volumes. Add 1.0mL of solu-
tion 6A, 6B, 6C, 6D, or 6E to each bottle containing 100.0mL of basal
medium. Add 0.1mL of solution 7, 0.1mL of solution 8, a...
Handbook of Microbiological Media, Fourth Edition part 166 doc
... For the differentiation of bacteria based on their ability to produce
glucan. Production of glucan is indicated when the broth is partially or
completely gelled—typical of Streptococcus sanguis—or ... on the bottom and walls of the tube—typi-
cal of Streptococcus mutans. An increase in the viscosity indicates the
production of slime (unknown polysaccharide)—typical of Streptococ...
Handbook of Microbiological Media, Fourth Edition part 1 pptx
... the numbers of particular species of
microorganisms. Most often, mixtures of growth factors are used in
microbiological media. Acid hydrolysates of casein commonly are used
as sources of amino acids. ... digest of soybean meal. It has a high vitamin and a high
carbohydrate content.
Polypeptone™ Peptone
A mixture of peptones composed of equal parts of pancreatic
digest of...
Handbook of Microbiological Media, Fourth Edition part 10 ppt
... 5.2g
Preparation of A & A FeEDTA Solution: Dissolve 5.2g of KOH
in 186.0mL of distilled/deionized water. Add 20.4g of disodium
EDTA·2H
2
O. Add 13.7g of FeSO4·7H2O to 364.0mL of distilled/deion-
ized ... pressure–121°C.
Preparation of Medium: Saturate cooled solution A under 100%
CO
2
at 0.05–0.1 atm pressure for 30 min with magnetic stirring. Add
860.0mL of solution B, 5...
Handbook of Microbiological Media, Fourth Edition part 17 pptx
... com-
bine 500.0mL of solution 1 with 500.0mL of solution 2.
Nutrient Agar:
Composition
per liter:
Agar 15.0g
Pancreatic digest of gelatin 5.0g
Beef extract 3.0g
Preparation of Nutrient Agar: ... 0.1g
Preparation of NAD Solution: Add NAD to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add 10.0g of agar to 700.0mL...
Handbook of Microbiological Media, Fourth Edition part 19 ppt
... 15 min at 15 psi pressure–121°C.
Preparation of Medium: Aseptically combine 500.0mL of sterile
solution A, 500.0mL of sterile solution C, and 1.0mL of sterile solution
B. Mix thoroughly. Pour ...
Preparation of Medium: Aseptically combine 1.0L of sterile solu-
tion A and 1.0mL of sterile solution B. Mix thoroughly. Aseptically
distribute into sterile tubes or flasks.
Use: For...
Handbook of Microbiological Media, Fourth Edition part 20 pptx
... fragilis group. For the differentiation of Bacteroides species
based on the hydrolysis of esculin and presence of catalase. After incu-
bation for 48 hr, bacteria of the Bacteroides fragilis group ... For the cultivation of a wide variety of bacteria.
BAGG Broth
(Buffered Azide Glucose Glycerol Broth)
Composition per liter:
Pancreatic digest of casein 10.0g
Peptic digest of a...
Handbook of Microbiological Media, Fourth Edition part 25 ppt
... liter:
Agar 20.0g
NaCl 5.5g
Pancreatic digest of casein 5.0g
Peptic digest of animal tissue 5.0g
Preparation of Bordet-Gengou Agar Base: Add components to
1.0L of 1% glycerol solution. Autoclave for ... 10.0g
NaCl 5.0g
Pancreatic digest of casein 5.0g
Peptic digest of animal tissue 5.0g
Charcoal 4.0g
Nicotinic acid 1.0mg
Preparation of Charcoal Agar Base: Add components of cha...
Handbook of Microbiological Media, Fourth Edition part 35 pptx
... 45°–50°C.
Preparation of Cellulose Overlay Agar: Aseptically combine
350.0mL of cooled, sterile solution A and 650.0mL of cooled, sterile
solution B. Mix thoroughly.
Preparation of Medium: Pour cooled, ... 0.25g
Na
2
S·9H
2
O 0.25g
Preparation of Alkaline Solution: Add components to 25.0mL of
distilled/deionized water. Mix thoroughly. Prepare freshly.
Preparation of Medium: Pre...
Handbook of Microbiological Media, Fourth Edition part 45 ppt
... from other coliform colonies
which have a salmon to red color. As part of an additional confirmation
of E. coli, the inclusion of tryptophan improves the indole reaction,
thereby increasing detection ... For the detection of E.coli and total coliforms. The combination
of suitable peptones and the buffering using MOPS allows rapid
growth of coliforms and an optimal transformation...
Từ khóa: