Handbook of Microbiological Media, Fourth Edition part 113 docx

... 10.0mL of sterile vi- tamin solution, 10.0mL of sterile NaHCO 3 solution, and 10.0mL of sterile Na 2 S·9H 2 O solution to each liter of medium or, using a syringe, inject the appropriate amount of ... ster- ilize. Preparation of Methanol: Filter sterilize 10.0mL of methanol. Na 2 S·9H 2 O Solution: Composition per 100.0mL: NaOH 1 pellet Na 2 S·9H 2 O 2.5g Preparation of Na 2...

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... 10.0mL of CaCl 2 solution, 10.0mL of MgSO 4 ·7H 2 O solution, 10.0mL of NaNO 3 solution, 10.0mL of K 2 HPO 4 solution, 10.0mL of KH 2 PO 4 solution, and 10.0mL of NaCl solution to distilled/deionized water ... 1.0g Preparation of FeCl 3 Solution: Add FeCl 3 to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Preparation of Bristol's Solution: A...

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... determination of phenol coefficients of disinfectant prod- ucts containing cationic surface-active materials. Use according to Official Methods of Analysis of the Association of Official Analytical Chemists ... inoculation of cultures, add 0.25mL of sterile Na 2 S·9H 2 O solution to each tube containing 9.75mL of sterile basal medium. Use: For the cultivation and maintenance...

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... and anaerobically add 50.0mL of sterile cellobiose solution, 10.0mL of sterile Wolfe’s mineral solutionn, 10.0mL of sterile Wolfe’s vitamin so- lution, and 10.0mL of sterile Na 2 S·9H 2 O solution. ... pressure–121°C. Aseptically and anaerobically add 10.0mL of sterile Wolfe’s mineral solution, 10.0mL of sterile Wolfe’s vitamin so- lution, and 10.0mL of sterile Na 2 S·9H 2 O sol...

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... Aseptically add 50.0mL of sterile blood and 1.5mL of sterile water-lysed blood solution (one part blood to three parts water). Water-lysed blood may be omitted if sterile blood is partially lysed due ... Aseptically add 50.0mL of sterile blood and 1.5mL of sterile water-lysed blood solution (one part blood to three parts water). Water-lysed blood may be omitted if sterile blood is...

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... 15.0mL Preparation of Part B: Prepare a 1M solution of K 2 CO 3 and filter sterilize. Filter sterilize 25.0mL of 95% ethanol solution. Aseptically combine 20.0mL of sterile K 2 CO 3 solution and 15.0mL of ... K 2 CO 3 solution and 15.0mL of sterile etha- nol. Preparation of Medium: Add 35.0mL of sterile Part B to 965.0mL of sterile, cooled Part A. Adjust pH to 7.0...

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... add 2.0mL of sterile tellurite soltuion and 50.0mL of sterile defibrinated blood. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation and maintenance of a wide variety of microor- ganisms. ... add 2.0mL of sterile tellurite soltuion and 50.0mL of sterile defibrinated blood. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation and main...

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... add 100.0mL of sterile glucose solution, 10.0mL of sterile Wolfe’s mineral solution, 10.0mL of sterile Wolfe’s vitamin solution, 10.0mL of sterile Na 2 S·9H 2 O solution, and 10.0mL of sterile ... Medium: To 80.0mL of cooled, sterile basal agar, aseptically add 20.0mL of sterile horse serum, 1.0mL of sterile fresh yeast extract solution, 1.0mL of sterile urea solution, 0.5mL...

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... of Solution C: Add NaHCO 3 to distilled/deionized water and bring volume to 14.0mL. Mix thoroughly. Sparge with 80% N 2 + 20% CO 2 . Filter sterilize. Preparation of Medium: Inject 1.0 ml of ... sterilize. Preparation of Medium: Inject 1.0 ml of solution B and 0.2 ml of solution C in each tube of solution A. Use: For the cultivation of Rhodovibrio sodomensis=Rhodospirillum...

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... pressure–121°C. Preparation of Medium: To each tube containing 8.9mL of sterile solution A, add (using a syringe) 1.0mL of sterile solution B, 0.1mL of sterile solution C, and 0.01mL of sterile solution ... order: 2.0mL of sterile menadione solution, 10.0mL of sterile L-cysteine·HCl·H 2 O solution, 10.0mL of sterile dithiothreitol solution, 10.0mL of sterile glucose solution...

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