Handbook of Microbiological Media, Fourth Edition part 100 potx
Handbook of Microbiological Media, Fourth Edition part 100 potx
... aseptically dilute 200.0mL of 5× stock solution with 789.0mL of sterile distilled/deionized water. Aseptically add 10.0mL of sterile carbohydrate solution and 1.0mL of sterile MgSO 4 ·7H 2 O solution. ... anaerobically add 20.0mL of Wolfe’s vitamin solution, 10.0mL of sterile NaHCO 3 solu- tion, 10.0mL of sterile Na 2 S·9H 2 O solution, 1.0mL Na 2 SeO 3 /Na 2 WO 4 solution, 1.0...
Ngày tải lên: 03/07/2014, 18:20
Handbook of Microbiological Media, Fourth Edition part 4 potx
... Solution: Composition per 100. 0mL: Glucose 40.0g Preparation of Glucose Solution: Add glucose to distilled/deion- ized water and bring volume to 100. 0mL. Mix thoroughly. Filter ster- ilize. Preparation of Medium: ... Acid: Filter sterilize 40.0mL of acetic acid using a teflon filter. Preparation of Ethanol: Filter sterilize 30.0mL of ethanol using a teflon filter. Preparati...
Ngày tải lên: 03/07/2014, 18:20
Handbook of Microbiological Media, Fourth Edition part 7 potx
... for 15 min at 15 psi pressure–121°C. Aseptically add 0.6mL of sterile raffinose solution, 0.2mL of sterile Na 2 CO 3 solution, and 0.2mL of sterile CoCl 2 ·6H 2 O solution to each tube. Mix thoroughly. ... 0.01g Penicillin G 100, 000 units Preparation of Selective Supplement Solution: Add compo- nents to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter st...
Ngày tải lên: 03/07/2014, 18:20
Handbook of Microbiological Media, Fourth Edition part 38 potx
... 7.0mL into tubes that contain meat particles (1 part meat parti- cles to 5 parts fluid). Autoclave for 30 min at 15 psi pressure–121°C. Use: For the cultivation of various anaerobes. Chopped Meat ... 7.0mL into tubes that contain meat particles (1 part meat particles to 5 parts fluid). Autoclave for 30 min at 15 psi pressure–121°C. Use: For the cultivation of numerous anaerobes, inclu...
Ngày tải lên: 03/07/2014, 18:20
Handbook of Microbiological Media, Fourth Edition part 48 potx
... MnCl 2 ·4H 2 O to 100. 0mL of dis- tilled/deionized water. Mix thoroughly. Solution D: Composition per 100. 0mL: ZnSO 4 ·7H 2 O 0.06g Preparation of Solution D: Add ZnSO 4 ·7H 2 O to 100. 0mL of dis- tilled/deionized ... 0.1mg Preparation of Solution B: Add VOSO 4 ·2H 2 O to 100. 0mL of dis- tilled/deionized water. Mix thoroughly. Solution C: Composition per 100. 0mL: MnCl...
Ngày tải lên: 03/07/2014, 18:20
Handbook of Microbiological Media, Fourth Edition part 69 potx
... pressure–121°C. Cool under an atmo- sphere of 100% N 2 . Aseptically add 9.0mL of trace elements solution and 5.0mL of Wolfe’s vitamin solution under an atmosphere of 100% N 2 . Mix thoroughly. Aseptically ... Preparation of Medium: To 916.0mL of sterile solution A, add 70.0mL of sterile solution B, 10.0mL of sterile solution C, 10.0mL of sterile solution D, and 10.0mL...
Ngày tải lên: 03/07/2014, 18:20
Handbook of Microbiological Media, Fourth Edition part 92 potx
... Sparge with 100% N 2 . Autoclave for 15 min at 15 psi pressure–121°C. Asep- tically and anaerobically add 100. 0mL of sterile glucose solution, 10.0mL of sterile NaHCO 3 solution, 10.0mL of sterile ... 70.0mL Preparation of Egg Yolk Emulsion: Soak eggs with 1 :100 dilu- tion of saturated mercuric chloride solution for 1 min. Crack 11 eggs and separate yolks from whites. Mix egg...
Ngày tải lên: 03/07/2014, 18:20
Handbook of Microbiological Media, Fourth Edition part 95 potx
... Agar Preparation of Rabbit Blood Solution: Add 5.0mL of whole rabbit blood to 5.0mL of sterile distilled/deionized water. Freeze and thaw twice to lyse blood cells. Preparation of Medium: Add components, ... Cool to 50°–56°C. Aseptical- ly add 100. 0mL of rabbit serum. Mix thoroughly. Use: For the cultivation of Leptospira species. Leptospira HiVeg Medium Base, Korthof, Modifie...
Ngày tải lên: 03/07/2014, 18:20
Handbook of Microbiological Media, Fourth Edition part 96 potx
... 1.0mg Preparation of Synthetic Seawater: Add components to dis- tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Preparation of Medium: Add 10.0g of pancreatic digest of casein to 1.0L of synthetic ... screening of blood specimens from suspected cases of bacteremia. Listeria Enrichment Broth Composition per liter: Pancreatic digest of casein 17.0g Yeast extrac...
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Handbook of Microbiological Media, Fourth Edition part 111 potx
... Fluid: Composition per 100. 0mL: Yeast extract 0.4g Sludge 100. 0mL Preparation of Sludge Fluid: To 100. 0mL of sludge from an an- aerobic digester, add 0.4g of yeast extract. Sparge with 100% N 2 for a few ... Fluid: Composition per 100. 0mL: Sludge 100. 0mL Yeast extract 0.4g Preparation of Sludge Fluid: To 100. 0mL of sludge from an an- aerobic digester, add 0.4g of y...
Ngày tải lên: 03/07/2014, 18:20