Handbook of Microbiological Media, Fourth Edition part 80 pptx
Handbook of Microbiological Media, Fourth Edition part 80 pptx
... with 80% N 2 + 20% CO 2 . Autoclave for 15 min at 15 psi pressure–121°C. Aseptically and anaerobically add 30.0mL of sterile glucose solution, 10.0mL of sterile NaHCO 3 solution, 10.0mL of sterile ... 50–55°C. Preparation of Medium: Aseptically combine 100.0mL of sterile solution B with 400.0mL of sterile solution C. Mix thoroughly. Adjust pH to 8.4. Aseptically add 500.0mL of...
Ngày tải lên: 03/07/2014, 18:20
Handbook of Microbiological Media, Fourth Edition part 1 pptx
... the numbers of particular species of microorganisms. Most often, mixtures of growth factors are used in microbiological media. Acid hydrolysates of casein commonly are used as sources of amino acids. ... digest of soybean meal. It has a high vitamin and a high carbohydrate content. Polypeptone™ Peptone A mixture of peptones composed of equal parts of pancreatic digest of...
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Handbook of Microbiological Media, Fourth Edition part 17 pptx
... com- bine 500.0mL of solution 1 with 500.0mL of solution 2. Nutrient Agar: Composition per liter: Agar 15.0g Pancreatic digest of gelatin 5.0g Beef extract 3.0g Preparation of Nutrient Agar: ... psi pressure–121°C. Pour into sterile Petri dishes. Preparation of Medium: Aseptically add 10.0mL of the vitamin solution to 980. 0mL of Sonneborn's base Paramecium medium. Mix tho...
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Handbook of Microbiological Media, Fourth Edition part 20 pptx
... fragilis group. For the differentiation of Bacteroides species based on the hydrolysis of esculin and presence of catalase. After incu- bation for 48 hr, bacteria of the Bacteroides fragilis group ... For the cultivation of a wide variety of bacteria. BAGG Broth (Buffered Azide Glucose Glycerol Broth) Composition per liter: Pancreatic digest of casein 10.0g Peptic digest of a...
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Handbook of Microbiological Media, Fourth Edition part 35 pptx
... 45°–50°C. Preparation of Cellulose Overlay Agar: Aseptically combine 350.0mL of cooled, sterile solution A and 650.0mL of cooled, sterile solution B. Mix thoroughly. Preparation of Medium: Pour cooled, ... 0.25g Na 2 S·9H 2 O 0.25g Preparation of Alkaline Solution: Add components to 25.0mL of distilled/deionized water. Mix thoroughly. Prepare freshly. Preparation of Medium: Pre...
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Handbook of Microbiological Media, Fourth Edition part 53 pptx
... combine 870.0mL of sterile solution A with 1.0mL of sterile solution B, 100.0mL of sterile solution C, 10.0mL of sterile solution D, 10.0mL of sterile solution E, and 10.0mL of sterile solution ... Medium: To 1.0L of sterile solution A, add in or- der: 1.0mL of sterile solution B, 2.0mL of sterile solution C, 50.0mL of sterile solution D, 10.0mL of sterile solution E, 1....
Ngày tải lên: 03/07/2014, 18:20
Handbook of Microbiological Media, Fourth Edition part 54 pptx
... combine 870.0mL of sterile solution A with 1.0mL of sterile solution B, 100.0mL of sterile solution C, 10.0mL of sterile solution D, 10.0mL of sterile solution E, and 10.0mL of sterile solution ... 5.0mg Riboflavin 5.0mg Thiamine·HCl 5.0mg Biotin 5.0mg Folic acid 5.0mg Vitamin B 12 5.0mg Hemin 5.0mg Preparation of Solution E (Vitamin Solution): Add 1,4-naphtho- quinone and hemi...
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Handbook of Microbiological Media, Fourth Edition part 55 pptx
... 1.0mL of sterile solution E, 1.0mL of sterile solution F, 10.0mL of sterile solution G, 1.0mL of sterile solution H, 6.6mL of sterile solution I, 20.0mL of sterile solution J, and 10.0mL of sterile ... 10.0mL of sterile solution D, 1.0mL of sterile solution E, 10.0mL of sterile solution F, 6.6mL of sterile solution G, 20.0mL of sterile solution H, and 10.0mL of steri...
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Handbook of Microbiological Media, Fourth Edition part 57 pptx
... To 1.0L of sterile solution A, add in the fol- lowing order: 1.0mL of sterile solution B, 2.0mL of sterile solution C, 50.0mL of sterile solution D, 10.0mL of sterile solution E, 1.0mL of ster- ile ... solution B, 100.0mL of sterile solution C, 10.0mL of sterile solution D, 10.0mL of sterile solution E, 6.6mL of sterile solution F, 20.0mL of sterile solution G, and 10.0m...
Ngày tải lên: 03/07/2014, 18:20
Handbook of Microbiological Media, Fourth Edition part 58 pptx
... freshly. Preparation of Medium: To 920.0mL of cooled, sterile solution A, aseptically and anaerobically add in the following order: 1.0mL of ster- ile solution B, 50.0mL of sterile solution C, 10.0mL of sterile ... anaerobically add 10.0mL of sterile Na 2 S·9H 2 O solution and 5.0g of sterile powdered sulfur. Mix thor- oughly. Final pH of medium should be 5.5. Use: For the cul...
Ngày tải lên: 03/07/2014, 18:20