Handbook of Microbiological Media, Fourth Edition part 76 docx
Handbook of Microbiological Media, Fourth Edition part 76 docx
... Red 0.5g
Preparation of Phenol Red Solution: Add Phenol Red to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Preparation of Medium: To 800.0mL of sterile solution B, ... add
20.0mL of sterile NaHCO
3
solution and 10.0mL of sterile Na
2
S·9H
2
O
solution. Mix thoroughly. Aseptically and anaerobically distribute into
sterile tubes or flasks.
Use: For the...
Handbook of Microbiological Media, Fourth Edition part 8 docx
... 10.0mL of CaCl
2
solution, 10.0mL of MgSO
4
·7H
2
O solution,
10.0mL of NaNO
3
solution, 10.0mL of K
2
HPO
4
solution, 10.0mL of
KH
2
PO
4
solution, and 10.0mL of NaCl solution to distilled/deionized
water ... 1.0g
Preparation of FeCl
3
Solution: Add FeCl
3
to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly.
Preparation of Bristol's Solution: A...
Handbook of Microbiological Media, Fourth Edition part 15 docx
... determination of phenol coefficients of disinfectant prod-
ucts containing cationic surface-active materials. Use according to
Official Methods of Analysis of the Association of Official Analytical
Chemists ... inoculation of cultures, add 0.25mL of
sterile Na
2
S·9H
2
O solution to each tube containing 9.75mL of sterile basal
medium.
Use: For the cultivation and maintenance...
Handbook of Microbiological Media, Fourth Edition part 18 docx
... and
anaerobically add 50.0mL of sterile cellobiose solution, 10.0mL of
sterile Wolfe’s mineral solutionn, 10.0mL of sterile Wolfe’s vitamin so-
lution, and 10.0mL of sterile Na
2
S·9H
2
O solution. ... pressure–121°C. Aseptically and anaerobically add 10.0mL of
sterile Wolfe’s mineral solution, 10.0mL of sterile Wolfe’s vitamin so-
lution, and 10.0mL of sterile Na
2
S·9H
2
O sol...
Handbook of Microbiological Media, Fourth Edition part 34 docx
... Aseptically add 50.0mL
of sterile blood and 1.5mL of sterile water-lysed blood solution (one
part blood to three parts water). Water-lysed blood may be omitted if
sterile blood is partially lysed due ... Aseptically add 50.0mL
of sterile blood and 1.5mL of sterile water-lysed blood solution (one
part blood to three parts water). Water-lysed blood may be omitted if
sterile blood is...
Handbook of Microbiological Media, Fourth Edition part 42 docx
... 15.0mL
Preparation of Part B: Prepare a 1M solution of K
2
CO
3
and filter
sterilize. Filter sterilize 25.0mL of 95% ethanol solution. Aseptically
combine 20.0mL of sterile K
2
CO
3
solution and 15.0mL of ... K
2
CO
3
solution and 15.0mL of sterile etha-
nol.
Preparation of Medium: Add 35.0mL of sterile Part B to 965.0mL
of sterile, cooled Part A. Adjust pH to 7.0...
Handbook of Microbiological Media, Fourth Edition part 60 docx
... add
2.0mL of sterile tellurite soltuion and 50.0mL of sterile defibrinated
blood. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of a wide variety of microor-
ganisms. ... add
2.0mL of sterile tellurite soltuion and 50.0mL of sterile defibrinated
blood. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and main...
Handbook of Microbiological Media, Fourth Edition part 61 docx
... add 100.0mL of sterile glucose solution,
10.0mL of sterile Wolfe’s mineral solution, 10.0mL of sterile Wolfe’s
vitamin solution, 10.0mL of sterile Na
2
S·9H
2
O solution, and 10.0mL
of sterile ... Medium: To 80.0mL of cooled, sterile basal agar,
aseptically add 20.0mL of sterile horse serum, 1.0mL of sterile fresh
yeast extract solution, 1.0mL of sterile urea solution, 0.5mL...
Handbook of Microbiological Media, Fourth Edition part 62 docx
... of Solution C: Add NaHCO
3
to distilled/deionized
water and bring volume to 14.0mL. Mix thoroughly. Sparge with 80%
N
2
+ 20% CO
2
. Filter sterilize.
Preparation of Medium: Inject 1.0 ml of ... sterilize.
Preparation of Medium: Inject 1.0 ml of solution B and 0.2 ml of
solution C in each tube of solution A.
Use: For the cultivation of Rhodovibrio sodomensis=Rhodospirillum...
Handbook of Microbiological Media, Fourth Edition part 67 docx
... pressure–121°C.
Preparation of Medium: To each tube containing 8.9mL of sterile
solution A, add (using a syringe) 1.0mL of sterile solution B, 0.1mL of
sterile solution C, and 0.01mL of sterile solution ... order: 2.0mL of sterile menadione solution, 10.0mL of sterile
L-cysteine·HCl·H
2
O solution, 10.0mL of sterile dithiothreitol solution,
10.0mL of sterile glucose solution...
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