Handbook of Microbiological Media, Fourth Edition part 55 pptx
Handbook of Microbiological Media, Fourth Edition part 55 pptx
... 1.0mL of sterile solution E, 1.0mL of sterile solution F, 10.0mL of
sterile solution G, 1.0mL of sterile solution H, 6.6mL of sterile solution
I, 20.0mL of sterile solution J, and 10.0mL of sterile ... 10.0mL of sterile solution
D, 1.0mL of sterile solution E, 10.0mL of sterile solution F, 6.6mL of
sterile solution G, 20.0mL of sterile solution H, and 10.0mL of steri...
Handbook of Microbiological Media, Fourth Edition part 1 pptx
... the numbers of particular species of
microorganisms. Most often, mixtures of growth factors are used in
microbiological media. Acid hydrolysates of casein commonly are used
as sources of amino acids. ... digest of soybean meal. It has a high vitamin and a high
carbohydrate content.
Polypeptone™ Peptone
A mixture of peptones composed of equal parts of pancreatic
digest of...
Handbook of Microbiological Media, Fourth Edition part 17 pptx
... com-
bine 500.0mL of solution 1 with 500.0mL of solution 2.
Nutrient Agar:
Composition
per liter:
Agar 15.0g
Pancreatic digest of gelatin 5.0g
Beef extract 3.0g
Preparation of Nutrient Agar: ... 0.1g
Preparation of NAD Solution: Add NAD to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add 10.0g of agar to 700.0mL...
Handbook of Microbiological Media, Fourth Edition part 20 pptx
... fragilis group. For the differentiation of Bacteroides species
based on the hydrolysis of esculin and presence of catalase. After incu-
bation for 48 hr, bacteria of the Bacteroides fragilis group ... For the cultivation of a wide variety of bacteria.
BAGG Broth
(Buffered Azide Glucose Glycerol Broth)
Composition per liter:
Pancreatic digest of casein 10.0g
Peptic digest of a...
Handbook of Microbiological Media, Fourth Edition part 35 pptx
... 45°–50°C.
Preparation of Cellulose Overlay Agar: Aseptically combine
350.0mL of cooled, sterile solution A and 650.0mL of cooled, sterile
solution B. Mix thoroughly.
Preparation of Medium: Pour cooled, ... 0.25g
Na
2
S·9H
2
O 0.25g
Preparation of Alkaline Solution: Add components to 25.0mL of
distilled/deionized water. Mix thoroughly. Prepare freshly.
Preparation of Medium: Pre...
Handbook of Microbiological Media, Fourth Edition part 53 pptx
... combine
870.0mL of sterile solution A with 1.0mL of sterile solution B,
100.0mL of sterile solution C, 10.0mL of sterile solution D, 10.0mL of
sterile solution E, and 10.0mL of sterile solution ... Medium: To 1.0L of sterile solution A, add in or-
der: 1.0mL of sterile solution B, 2.0mL of sterile solution C, 50.0mL
of sterile solution D, 10.0mL of sterile solution E, 1....
Handbook of Microbiological Media, Fourth Edition part 54 pptx
... combine
870.0mL of sterile solution A with 1.0mL of sterile solution B,
100.0mL of sterile solution C, 10.0mL of sterile solution D, 10.0mL of
sterile solution E, and 10.0mL of sterile solution ... 5.0mg
Riboflavin 5.0mg
Thiamine·HCl 5.0mg
Biotin 5.0mg
Folic acid 5.0mg
Vitamin B
12
5.0mg
Hemin 5.0mg
Preparation of Solution E (Vitamin Solution): Add 1,4-naphtho-
quinone and hemi...
Handbook of Microbiological Media, Fourth Edition part 57 pptx
... To 1.0L of sterile solution A, add in the fol-
lowing order: 1.0mL of sterile solution B, 2.0mL of sterile solution C,
50.0mL of sterile solution D, 10.0mL of sterile solution E, 1.0mL of ster-
ile ... solution B,
100.0mL of sterile solution C, 10.0mL of sterile solution D, 10.0mL of
sterile solution E, 6.6mL of sterile solution F, 20.0mL of sterile solution G,
and 10.0m...
Handbook of Microbiological Media, Fourth Edition part 58 pptx
... freshly.
Preparation of Medium: To 920.0mL of cooled, sterile solution A,
aseptically and anaerobically add in the following order: 1.0mL of ster-
ile solution B, 50.0mL of sterile solution C, 10.0mL of sterile ... anaerobically add 10.0mL of sterile
Na
2
S·9H
2
O solution and 5.0g of sterile powdered sulfur. Mix thor-
oughly. Final pH of medium should be 5.5.
Use: For the cul...
Handbook of Microbiological Media, Fourth Edition part 70 pptx
... psi pressure–121°C. Cool to 45°–50°C.
Aseptically add 10.0mL of sterile vitamin solution, 10.0mL of sterile
glucose solution, and 1.0mL of sterile trace elments solution. Mix thor-
oughly. Pour ... 20.0g
Pancreatic digest of casein 5.0g
Peptic digest of animal tissue 5.0g
pH5.7 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparat...
Từ khóa: