... 1.0mL of sterile solution E, 1.0mL of sterile solution F, 10.0mL of sterile solution G, 1.0mL of sterile solution H, 6.6mL of sterile solutionI, 20.0mL of sterile solution J, and 10.0mL of sterile ... 10.0mL of sterile solutionD, 1.0mL of sterile solution E, 10.0mL of sterile solution F, 6.6mL of sterile solution G, 20.0mL of sterile solution H, and 10.0mL of sterilesolution I. Mix thoroughly. ... solu-tion.Preparation of Medium: To 890.0mL of cooled, sterile solution A,aseptically and anaerobically add in the following order: 1.0mL of ster-ile solution B, 50.0mL of sterile solution C, 10.0mL of sterile...