Handbook of Microbiological Media, Fourth Edition part 16 pot
Handbook of Microbiological Media, Fourth Edition part 16 pot
... to 45°–50°C. Preparation of Medium: To 1.0L of sterile Eagle’s basal medium, aseptically add 1.0L of sterile, cooled agarose solution and 100.0mL of fetal calf serum. Mix thoroughly. Pour into ... per 100.0mL: Tris[hydroxymethyl]aminomethane buffer 25.0g Preparation of Tris Buffer Solution: Add 25.0g of tris to 65.0mL of distilled/deionized water. Titrate to pH 7.6–7.7 with con...
Ngày tải lên: 03/07/2014, 18:20
Handbook of Microbiological Media, Fourth Edition part 4 potx
... Acid: Filter sterilize 40.0mL of acetic acid using a teflon filter. Preparation of Ethanol: Filter sterilize 30.0mL of ethanol using a teflon filter. Preparation of Medium: Add components, except ... anaerobically add 0.1mL of sterile glucose, 0.1mL of sterile L-cysteine·HCl·H 2 O solution, and 0.1mL of sterile Na 2 S·9H 2 O solution to each tube. Mix thoroughly. Use: For th...
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Handbook of Microbiological Media, Fourth Edition part 7 potx
... of Acid Fuchsin Solution: Add Acid Fuchsin to 50.0mL of 5% aqueous dioxan. Mix well to dissolve. Caution: Acid Fuchsin is a potential carcinogen and care must be tak- en to avoid inhalation of ... for 15 min at 15 psi pressure–121°C. Aseptically add 0.6mL of sterile raffinose solution, 0.2mL of sterile Na 2 CO 3 solution, and 0.2mL of sterile CoCl 2 ·6H 2 O solution to each tube....
Ngày tải lên: 03/07/2014, 18:20
Handbook of Microbiological Media, Fourth Edition part 9 pot
... of Medium: Aseptically combine 900.0mL of solu- tion A with 100.0mL of solution B. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation and maintenance of ... elements solution 20.0mL Preparation of Base Solution: Sparge 2.0L of distilled/deionized water with 100% N 2 to remove O 2 . Add components to 2000.0mL of O 2 - free distilled/deioni...
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Handbook of Microbiological Media, Fourth Edition part 22 pot
... 100.0mL of sterile vitamin B 12 solution. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation and maintenance of Synechococcus species. For the isolation of ... 100.0mL of sterile vitamin B 12 solution. Mix thoroughly. Distribute into sterile tubes or flasks. Use: For the cultivation and maintenance of Synechococcus species. For the isolatio...
Ngày tải lên: 03/07/2014, 18:20
Handbook of Microbiological Media, Fourth Edition part 23 pot
... 45°–50°C. Aseptically add 100.0mL of sterile asparagine solution, 100.0mL of ster- ile methionine solution, 100.0mL of sterile sorbose solution, 10.0mL of sterile yeast extract solution, 10.0mL of sterile sodium ... Nitrofurantoin Solution: Composition per 10.0mL: Sodium nitrofurantoin 0.01g Preparation of Sodium Nitrofurantoin Solution: Add sodium nitrofurantoin to distilled/dei...
Ngày tải lên: 03/07/2014, 18:20
Handbook of Microbiological Media, Fourth Edition part 36 pot
... isolation of various bacteria; with the addition of blood, for the cultivation of fastidious bacteria. For the cul- tivation of Bordetella pertussis for vaccine production and the mainte- nance of stock ... isolation of various bacteria; with the addition of blood, for the cultivation of fastidious bacteria. For the cul- tivation of Bordetella pertussis for vaccine productio...
Ngày tải lên: 03/07/2014, 18:20
Handbook of Microbiological Media, Fourth Edition part 38 potx
... 7.0mL into tubes that contain meat particles (1 part meat parti- cles to 5 parts fluid). Autoclave for 30 min at 15 psi pressure–121°C. Use: For the cultivation of various anaerobes. Chopped Meat ... 7.0mL into tubes that contain meat particles (1 part meat particles to 5 parts fluid). Autoclave for 30 min at 15 psi pressure–121°C. Use: For the cultivation of numerous anaerobes, inclu...
Ngày tải lên: 03/07/2014, 18:20
Handbook of Microbiological Media, Fourth Edition part 43 pot
... pressure–121°C. Preparation of Medium: To each tube containing 8.9mL of sterile solution A, add (using a syringe) 1.0mL of sterile solution B, 0.1mL of sterile solution C, and 0.01mL of sterile solution ... anaerobically add 100.0mL of sterile phosphate solution, 100.0mL of sterile glucose so- lution, 100.0mL of sterile NaHCO 3 solution, 10.0mL of sterile L- cysteine·HCl so...
Ngày tải lên: 03/07/2014, 18:20
Handbook of Microbiological Media, Fourth Edition part 48 potx
... 6.0mL of IM with the addition of 0.001mg/mL cycloheximide (final concentration)) and add 0.5– 1.0mL of EB stock solution (thawed quickly to 37°C). Centrifuge for 1 h onto the cell layer at 160 0 ... 20.0g Pancreatic digest of casein 20.0g MgSO 4 ·7H 2 O 2.0g Potassium phosphate buffer (0.02M solution, pH 7.6) 500.0mL pH 7.6 ± 0.2 at 25°C Preparation of Medium: Add agar, pancreatic...
Ngày tải lên: 03/07/2014, 18:20