Handbook of Microbiological Media, Fourth Edition part 16 pot
Handbook of Microbiological Media, Fourth Edition part 16 pot
... to 45°–50°C.
Preparation of Medium: To 1.0L of sterile Eagle’s basal medium,
aseptically add 1.0L of sterile, cooled agarose solution and 100.0mL of
fetal calf serum. Mix thoroughly. Pour into ... per 100.0mL:
Tris[hydroxymethyl]aminomethane buffer 25.0g
Preparation of Tris Buffer Solution: Add 25.0g of tris to 65.0mL of
distilled/deionized water. Titrate to pH 7.6–7.7 with con...
Handbook of Microbiological Media, Fourth Edition part 4 potx
... Acid: Filter sterilize 40.0mL of acetic acid
using a teflon filter.
Preparation of Ethanol: Filter sterilize 30.0mL of ethanol using a
teflon filter.
Preparation of Medium: Add components, except ... anaerobically add 0.1mL of sterile
glucose, 0.1mL of sterile
L-cysteine·HCl·H
2
O solution, and 0.1mL of
sterile Na
2
S·9H
2
O solution to each tube. Mix thoroughly.
Use: For th...
Handbook of Microbiological Media, Fourth Edition part 7 potx
... of Acid Fuchsin Solution: Add Acid Fuchsin to
50.0mL of 5% aqueous dioxan. Mix well to dissolve.
Caution: Acid Fuchsin is a potential carcinogen and care must be tak-
en to avoid inhalation of ... for 15 min at 15 psi
pressure–121°C. Aseptically add 0.6mL of sterile raffinose solution,
0.2mL of sterile Na
2
CO
3
solution, and 0.2mL of sterile CoCl
2
·6H
2
O
solution to each tube....
Handbook of Microbiological Media, Fourth Edition part 9 pot
... of Medium: Aseptically combine 900.0mL of solu-
tion A with 100.0mL of solution B. Mix thoroughly. Pour into sterile
Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of ... elements solution 20.0mL
Preparation of Base Solution: Sparge 2.0L of distilled/deionized
water with 100% N
2
to remove O
2
. Add components to 2000.0mL of O
2
-
free distilled/deioni...
Handbook of Microbiological Media, Fourth Edition part 22 pot
... 100.0mL of sterile vitamin B
12
solution.
Mix thoroughly. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Synechococcus species.
For the isolation of ... 100.0mL of sterile vitamin B
12
solution.
Mix thoroughly. Distribute into sterile tubes or flasks.
Use: For the cultivation and maintenance of Synechococcus species.
For the isolatio...
Handbook of Microbiological Media, Fourth Edition part 23 pot
... 45°–50°C.
Aseptically add 100.0mL of sterile asparagine solution, 100.0mL of ster-
ile methionine solution, 100.0mL of sterile sorbose solution, 10.0mL of
sterile yeast extract solution, 10.0mL of sterile sodium ... Nitrofurantoin Solution:
Composition
per 10.0mL:
Sodium nitrofurantoin 0.01g
Preparation of Sodium Nitrofurantoin Solution: Add sodium
nitrofurantoin to distilled/dei...
Handbook of Microbiological Media, Fourth Edition part 36 pot
... isolation of various bacteria; with the
addition of blood, for the cultivation of fastidious bacteria. For the cul-
tivation of Bordetella pertussis for vaccine production and the mainte-
nance of stock ... isolation of various bacteria; with the
addition of blood, for the cultivation of fastidious bacteria. For the cul-
tivation of Bordetella pertussis for vaccine productio...
Handbook of Microbiological Media, Fourth Edition part 38 potx
... 7.0mL into tubes that contain meat particles (1 part meat parti-
cles to 5 parts fluid). Autoclave for 30 min at 15 psi pressure–121°C.
Use: For the cultivation of various anaerobes.
Chopped Meat ... 7.0mL into
tubes that contain meat particles (1 part meat particles to 5 parts fluid).
Autoclave for 30 min at 15 psi pressure–121°C.
Use: For the cultivation of numerous anaerobes, inclu...
Handbook of Microbiological Media, Fourth Edition part 43 pot
... pressure–121°C.
Preparation of Medium: To each tube containing 8.9mL of sterile
solution A, add (using a syringe) 1.0mL of sterile solution B, 0.1mL of
sterile solution C, and 0.01mL of sterile solution ... anaerobically add
100.0mL of sterile phosphate solution, 100.0mL of sterile glucose so-
lution, 100.0mL of sterile NaHCO
3
solution, 10.0mL of sterile L-
cysteine·HCl so...
Handbook of Microbiological Media, Fourth Edition part 48 potx
... 6.0mL of IM with the addition
of 0.001mg/mL cycloheximide (final concentration)) and add 0.5–
1.0mL of EB stock solution (thawed quickly to 37°C). Centrifuge for
1 h onto the cell layer at 160 0 ... 20.0g
Pancreatic digest of casein 20.0g
MgSO
4
·7H
2
O 2.0g
Potassium phosphate buffer (0.02M solution, pH 7.6) 500.0mL
pH 7.6 ± 0.2 at 25°C
Preparation of Medium: Add agar, pancreatic...
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