Molecular Biology Problem Solver 52 doc

... 4.2 Centrifuge Application Guide Low Speed High Speed Ultra Application (70 00 rpm/ (30,000 rpm/ (100,000 rpm/ for Pelleting 70 00 ¥ g) 100,000 ¥ g) 1,000,000 ¥ g) Cells Yes Yes Feasible, but not recommended Nuclei ... the Visible Range? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 07 What Are the Strategies to Determine the Extinction Coefficient of a Compoun...

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... How to Properly Use and Maintain Laboratory Equipment 79 trical voltage to the overall measurement system. Generally this is a minor error. If the flow ... The fill solution most often contains potassium chloride, but incompatibility with some Figure 4 .9 Double-junction combination electrode. Rep- roduced with permission from Thermo Orion Inc. How ... liquid surface of the weighing vessel sh...

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... E., Moore, D. D., Seidman, J. G., Smith, J. A., and Struhl, K., eds., Current Protocols in Molecular Biology. Wiley, New York, pp. 10.1.1–10.1.3. US Pharmacopeia, USP645. Voet, D., and Voet, J. ... for them to check with their physician as to the potential hazards to them of working in a microbiology lab where even organisms that are normally nonpathogenic are being handled. The Inoculati...

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... Preparation . . . 212 What Constitutes “RNase-Free Technique”? . . . . . . . . . . . . 212 Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 2001 by Wiley-Liss, ... Reused? . . . 211 Can a Kit Designed to Isolate mRNA Directly from the Biological Sample Purify mRNA from Total RNA? . . . 212 Maximizing the Yield and Quality of an RNA Pr...

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... Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G. and Struhl, K. 1995. Current Protocols in Molecular Biology. Wiley, New York. Bashyam, M., and Tyage, A. 1994. An efficient and high yielding method ... method for iso- lation of RNA from Mycobacteria. Biotech. 17:834–836. Brown, T. A., ed. 1991. Molecular Biology Labfax. Bios Scientific Publishers, Oxford, U.K. Cheung, A. L., Eberhar...

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... . . . . . . . . . . . . . . . . . . . . . . 241 Double Digests: Simultaneous or Sequential? . . . . . . . . . . . . 242 Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. ... mycoparasitism. Microbiol. 143:3157–3164. Rapley, R., and Manning, D. L. 1998. Methods in Molecular Biology: RNA Isolation and Characterization Protocols, vol. 86. Humana Press, Totowa,...

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... enzymes are active, and the restriction sites are within several bases of each other, there may be a problem cutting close to the end of the fragment. Sequential Enzyme sets that are not compatible ... megabase mapping can be found in Nelson and McClelland (1992) and Qiang et al. (1990). A potential problem is that certain methyla- tion sites may react slowly allowing partial cleavage eve...

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... . . . . . . . . . . . . . . . . . . . . . . . 309 How Can Sample Preparation Affect Your Results? . . . . . . . 311 291 Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. ... appropriate PCR strategy, to avoid problems, and to help you think your way out of problems that do arise. While your research topic may be unique, the solutions to most PCR problems are les...

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... . . . . . . . . . . . . . . . 385 Amplification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 387 Molecular Biology Problem Solver: A Laboratory Guide. Edited ... markers will provide precise molecular weight values. The pre-stained recombinant proteins of known, reproducible molecular weights discussed above can also determine the molecular...

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... . . . . . . 475 What Are the Options for Cloning a Gene for Expression? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 475 Molecular Biology Problem Solver: A Laboratory ... . . . . . . . . . . . 472 Is It Necessary to Cleave the Tag off the Fusion Protein? 474 Will Extra Amino Acid Residues Affect Your Protein of Interest after Digestion? 475...

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... England Biolabs. In this latter case fusion proteins with chitin-binding domain are bound to high molecular weight chitin chromatography media and incubated in the presence of a reducing agent, ... at the amino terminal of the protein of interest following cleavage. Whether or not this poses a problem depends entirely on the protein and the use to which it will be put. Even the most dema...

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... obtain a known gene of interest include purchase from a distributor of clones from the Integrated Molecular Analysis of Genomes and their Expression (IMAGE) consortium (http://image.llnl.gov/), ... inability to obtain homogeneously pure protein for crys- tallization is a frequently encountered problem due to the het- erogeneous carbohydrate content of many eukaryotic proteins (Gruening...

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... for comparative purposes, and the numbers indicate how much stronger these promoters are. This problem is compounded by the tendency of overexpressing cells to grow slower than low or nonexpressors. In ... include the Ecdysone-inducible system, based on the heterodimeric ecdysone Eukaryotic Expression 509 Molecular Biochemicals, a combination of tiamulin and minocy- cline) (Uphoff, Gignac...

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... transient assays (e.g., in COS cells). Once you have ruled out problems with the expression con- struct, there are a few obvious places where problems may be occurring. First, one could perform Northern ... sequences. In some cases an overlooked point mutation or mistake in the original design is the problem. Ideally such prob- lems are best uncovered before weeks of work have been devoted t...

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