Molecular Biology Problem Solver 51 docx

... obtain a known gene of interest include purchase from a distributor of clones from the Integrated Molecular Analysis of Genomes and their Expression (IMAGE) consortium (http://image.llnl.gov/), ... inability to obtain homogeneously pure protein for crys- tallization is a frequently encountered problem due to the het- erogeneous carbohydrate content of many eukaryotic proteins (Gruening...

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... the sample is not allowed to reach the temperature of the weighing instrument. This is especially problematic when weighing very small samples. You the researcher are also a source of moisture that ... in the form of fingerprints and body oils. How to Properly Use and Maintain Laboratory Equipment 51 convection currents at the surface. Cold samples will appear heavier, and hot samples will b...

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... movement develops at the junction contributing a small elec- Table 4.4 Pipette Troubleshooting Guide Problem Possible Cause Solution Pipette drips or Tip is loose or does Use manufacturer recommended leaks ... section on pipetting technique technique you can reduce the amount of time redoing experiments, if a problem is found. How Can You Monitor the Performance of a Pipette? All of the i...

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... E., Moore, D. D., Seidman, J. G., Smith, J. A., and Struhl, K., eds., Current Protocols in Molecular Biology. Wiley, New York, pp. 10.1.1–10.1.3. US Pharmacopeia, USP645. Voet, D., and Voet, J. ... for them to check with their physician as to the potential hazards to them of working in a microbiology lab where even organisms that are normally nonpathogenic are being handled. The Inoculati...

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... enzymes are active, and the restriction sites are within several bases of each other, there may be a problem cutting close to the end of the fragment. Sequential Enzyme sets that are not compatible ... megabase mapping can be found in Nelson and McClelland (1992) and Qiang et al. (1990). A potential problem is that certain methyla- tion sites may react slowly allowing partial cleavage eve...

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... . . . . 309 How Can Sample Preparation Affect Your Results? . . . . . . . 311 291 Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 2001 by Wiley-Liss, ... appropriate PCR strategy, to avoid problems, and to help you think your way out of problems that do arise. While your research topic may be unique, the solutions to most PCR problems are le...

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... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 387 Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 2001 by Wiley-Liss, ... markers will provide precise molecular weight values. The pre-stained recombinant proteins of known, reproducible molecular weights discussed above can also determine the molecular we...

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... expression vector and the con- Eukaryotic Expression 517 firmed sequences. In some cases an overlooked point mutation or mistake in the original design is the problem. Ideally such prob- lems are best uncovered ... transient assays (e.g., in COS cells). Once you have ruled out problems with the expression con- struct, there are a few obvious places where problems may be occurring. First, on...

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... cuvettes, 100 for pH meters, 86 for spectrophotometers, 95 Scales, 51 55 calibrating, 55 Scaling up of eukaryotic expression, 514 515 , 527 of gene expression, 478–479 Index 573 Templates, troubleshooting ... conditions, as source of problems, 21–22, 40–42 Storage phosphor imagers dynamic range of, 442–443 erasure of, 447 in nucleic acid hybridization, 441–448 operation of, 441 problems wit...

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