Molecular Biology Problem Solver 49 pot

... conditions, as source of problems, 21–22, 40–42 Storage phosphor imagers dynamic range of, 442–443 erasure of, 447 in nucleic acid hybridization, 441–448 operation of, 441 problems with, 447–448 quantitative ... 350–353 native PAGE versus, 345–348 problems with, 480–481 standardized gels for, 363 Sealed containment rooms, in biosafety, 117 Secondary antibodies, 384 problems with, 395 Secondar...

Ngày tải lên: 02/07/2014, 04:21

... MOLECULAR BIOLOGY PROBLEM SOLVER Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 20 01 by Wiley-Liss, Inc. ISBNs: 0-4 71- 37972-7 (Paper); 0-4 71- 22390-5 ... Permissions Department, John Wiley & Sons, Inc., 605 Third Avenue, New York, NY 10 158-0 012 , ( 212 ) 850-6 011 , fax ( 212 ) 850-6008, E-Mail: PERMREQ@WILEY.COM. This p...

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... . . . . . . . . 9 Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 20 01 by Wiley-Liss, Inc. ISBNs: 0-471-379 72- 7 (Paper); 0-471 -22 390-5 (Electronic) nique ... Project Will Define You 2 Which Research Style Best Fits Your Situation? . . . . . . . . . . 2 Do You Have the Essential Resources? . . . . . . . . . . . . . . . . . . 2 Expe...

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... . . . . . . . 19 Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 2001 by Wiley-Liss, Inc. ISBNs: 0-471 -37 972-7 (Paper); 0-471-2 239 0-5 (Electronic) Big ... . . . . . . . . . . . . . 13 Does a Company Test Every Application for a Product? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 How Well Will...

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... the wrong temperature. Solving Problems by Yourself In a perfect world, six steps will solve any problem: 1. Define the problem. What do you see? The first step in any problem- solving activity is ... nature of the problem without drawing conclusions. Understanding a problem consists of describing all the factual aspects about the problem. Do not try to determine what caused the probl...

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... Properly Use and Maintain Laboratory Equipment 61 68 Troutman et al. adjustable pipette, set at 100 ml has an inaccuracy specification of ±0 .8% , whereas a Series 2100 100 ml fixed-volume pipettes ... move freely on the piston. Overall pipette design is similar from one brand to the next (Figure 4 .8) , but each pipette has a few slightly different features. Always refer to the manufacturer’...

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... Moore, D. D., Seidman, J. G., Smith, J. A., and Struhl, K. (eds.), 1998. Current Protocols in Molecular Biology. Wiley, New York. Beckman-Coulter Corporation. Application Note A—1790A. 1995. Biochrom ... 1988). 108 Troutman et al. Smith (1987) lists compounds that interfere with each assay and illustrates problems associated with the use of BSA as a standard (see also Harlow and Lane, 198...

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... environmental contamination persists, work in a laminar flow biosafety hood. If a contamination problem persists in the lab, consider an examination of the lab’s air delivery system. Placing filters ... it’s a trade-off between that and soaking the plate with the condensation. In the clinical microbiology lab, blood agar plates that contain cultures under evaluation are stored at the benchtop...

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... avoided because it may increase primer-dimer formation and reduce PCR effi- ciency. This is more problematic when you have a low number of target gene copies. • Avoid runs of G/C, especially guanidine. • When ... have the correct sequence. Inefficient desalting, incorrect labeling, and other quality control problems can ruin a primer’s performance. 314 Aoyagi which increases specificity of the...

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... principle here is that impurities in the water cause problems, and the purest water avail- able should be used for electrophoresis to help prevent these problems. Bacteria in your water purifier can also ... not controlled, the pore size will vary from day to day. Symptoms of Problems with Catalyst Potency The best indicator of a problem catalyst is poor polymeriza- tion of the gel. If you’...

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... 1997; Molloy, 2000).Very low and very high molecular weight proteins may also be problematic, as well as basic proteins. Procedures to avoid these problems must be worked out for each sample. How ... every gel. Is the Problem Caused by the Sample or the Sample Buffer? For lyophilized standards, make fresh standard buffer. Some- times it is difficult to determine whether the problem is i...

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... can be used to report only approximate molecular weights (within 10,000 daltons of the molecular weight as determined by an unstained standard). The molecular weight values of most pre-stained ... to Determine the Molecular Weight of an Unknown Protein? Pre-stained protein standards usually run as broad, fuzzy bands, making them useful for approximate, but not exact, molecular we...

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... . . . . . . . . . . . . . . . . . . . . . 412 Is It Necessary to Purify Every Probe? . . . . . . . . . . . . . . . . . 413 Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan ... . . . . . . . . . 441 Detection by Storage Phosphor Imagers . . . . . . . . . . . . . . . . . . 441 How Do Phosphor Imagers Work? . . . . . . . . . . . . . . . . . . . . . 441 Is a Stora...

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... loading buffer, DNaseI (10mg/ml), extended heating of the sample, or sonication should alleviate the problem. After electrophoresis, the gel should be stained (e.g., Coomassie Blue) to visualize the ... whole cell lysate. If expres- sion is good, an induced band will clearly be seen at the predicted molecular weight, and this will be absent in the no-insert control culture. If no band is...

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