Molecular Biology Problem Solver 48 doc

... England Biolabs. In this latter case fusion proteins with chitin-binding domain are bound to high molecular weight chitin chromatography media and incubated in the presence of a reducing agent, ... at the amino terminal of the protein of interest following cleavage. Whether or not this poses a problem depends entirely on the protein and the use to which it will be put. Even the most dema...

Ngày tải lên: 02/07/2014, 04:21

... the sample is not allowed to reach the temperature of the weighing instrument. This is especially problematic when weighing very small samples. You the researcher are also a source of moisture that ... moisture (up to 400 micrograms [mg]). The body will also transfer heat to the sample, creating problems addressed above. Sample Location The sample should be centered as much as possible on the...

Ngày tải lên: 02/07/2014, 04:21

... button and pull the tip up along the inside of the weighing vessel. Release the control button. 4. Document the value that appears in the display of the balance immediately after the display has ... movement develops at the junction contributing a small elec- Table 4.4 Pipette Troubleshooting Guide Problem Possible Cause Solution Pipette drips or Tip is loose or does Use manufacturer recomm...

Ngày tải lên: 02/07/2014, 04:21

... E., Moore, D. D., Seidman, J. G., Smith, J. A., and Struhl, K., eds., Current Protocols in Molecular Biology. Wiley, New York, pp. 10.1.1–10.1.3. US Pharmacopeia, USP645. Voet, D., and Voet, J. ... for them to check with their physician as to the potential hazards to them of working in a microbiology lab where even organisms that are normally nonpathogenic are being handled. The Inoculati...

Ngày tải lên: 02/07/2014, 04:21

... Preparation . . . 212 What Constitutes “RNase-Free Technique”? . . . . . . . . . . . . 212 Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 2001 by Wiley-Liss, ... 1989. Molecular Cloning: A Labora- tory Manual, 2nd ed. Cold Spring Laboratory, Cold Spring Harbor, NY. Schratter, P., Krowczynska, A. M., and Leonard, J. T. 1993. Ultrafiltration...

Ngày tải lên: 02/07/2014, 04:21

... Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G. and Struhl, K. 1995. Current Protocols in Molecular Biology. Wiley, New York. Bashyam, M., and Tyage, A. 1994. An efficient and high yielding method ... method for iso- lation of RNA from Mycobacteria. Biotech. 17:834–836. Brown, T. A., ed. 1991. Molecular Biology Labfax. Bios Scientific Publishers, Oxford, U.K. Cheung, A. L., Eberhar...

Ngày tải lên: 02/07/2014, 04:21

... . . . 241 Double Digests: Simultaneous or Sequential? . . . . . . . . . . . . 242 Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 2001 by Wiley-Liss, ... mycoparasitism. Microbiol. 143:3157–3164. Rapley, R., and Manning, D. L. 1998. Methods in Molecular Biology: RNA Isolation and Characterization Protocols, vol. 86. Humana Press, Totowa,...

Ngày tải lên: 02/07/2014, 04:21

... enzymes are active, and the restriction sites are within several bases of each other, there may be a problem cutting close to the end of the fragment. Sequential Enzyme sets that are not compatible ... megabase mapping can be found in Nelson and McClelland (1992) and Qiang et al. (1990). A potential problem is that certain methyla- tion sites may react slowly allowing partial cleavage eve...

Ngày tải lên: 02/07/2014, 04:21

... . . . . 309 How Can Sample Preparation Affect Your Results? . . . . . . . 311 291 Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 2001 by Wiley-Liss, ... appropriate PCR strategy, to avoid problems, and to help you think your way out of problems that do arise. While your research topic may be unique, the solutions to most PCR problems are le...

Ngày tải lên: 02/07/2014, 04:21

... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 387 Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 2001 by Wiley-Liss, ... markers will provide precise molecular weight values. The pre-stained recombinant proteins of known, reproducible molecular weights discussed above can also determine the molecular we...

Ngày tải lên: 02/07/2014, 04:21