Molecular Biology Problem Solver 47 doc
Molecular Biology Problem Solver 47 doc
... . . . . . . 475
What Are the Options for Cloning a Gene for
Expression? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 475
Molecular Biology Problem Solver: A Laboratory ... . . . . . . . . . . . 472
Is It Necessary to Cleave the Tag off the Fusion
Protein? 474
Will Extra Amino Acid Residues Affect Your Protein of
Interest after Digestion? 475...
Molecular Biology Problem Solver 7 docx
... the sample is not allowed to reach the temperature
of the weighing instrument. This is especially problematic when
weighing very small samples. You the researcher are also a source
of moisture that ... moisture (up to 400 micrograms [mg]). The body will
also transfer heat to the sample, creating problems addressed
above.
Sample Location
The sample should be centered as much as possible on the...
Molecular Biology Problem Solver 9 docx
... button and pull the tip up along the
inside of the weighing vessel. Release the control button.
4. Document the value that appears in the display of the
balance immediately after the display has ... movement develops at the junction contributing a small elec-
Table 4.4 Pipette Troubleshooting Guide
Problem Possible Cause Solution
Pipette drips or Tip is loose or does Use manufacturer recomm...
Molecular Biology Problem Solver 13 docx
... E., Moore, D. D., Seidman, J. G., Smith, J. A., and Struhl, K., eds., Current
Protocols in Molecular Biology. Wiley, New York, pp. 10.1.1–10.1.3.
US Pharmacopeia, USP645.
Voet, D., and Voet, J. ... for them to check with their physician as to
the potential hazards to them of working in a microbiology lab
where even organisms that are normally nonpathogenic are being
handled.
The Inoculati...
Molecular Biology Problem Solver 21 doc
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Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein
Copyright © 2001 by Wiley-Liss, Inc.
ISBNs: 0 -471 -37972-7 (Paper); 0 -471 -22390-5 (Electronic)
How ... 1989. Molecular Cloning: A Labora-
tory Manual, 2nd ed. Cold Spring Laboratory, Cold Spring Harbor, NY.
Schratter, P., Krowczynska, A. M., and Leonard, J. T. 1993. Ultrafiltration...
Molecular Biology Problem Solver 23 doc
... Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G. and Struhl,
K. 1995. Current Protocols in Molecular Biology. Wiley, New York.
Bashyam, M., and Tyage, A. 1994. An efficient and high yielding method ... method for iso-
lation of RNA from Mycobacteria. Biotech. 17:834–836.
Brown, T. A., ed. 1991. Molecular Biology Labfax. Bios Scientific Publishers,
Oxford, U.K.
Cheung, A. L., Eberhar...
Molecular Biology Problem Solver 24 doc
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Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein
Copyright © 2001 by Wiley-Liss, Inc.
ISBNs: 0 -471 -37972-7 (Paper); 0 -471 -22390-5 (Electronic)
and ... mycoparasitism. Microbiol.
143:3157–3164.
Rapley, R., and Manning, D. L. 1998. Methods in Molecular Biology: RNA
Isolation and Characterization Protocols, vol. 86. Humana Press,...
Molecular Biology Problem Solver 26 docx
... enzymes are active, and the restriction sites are
within several bases of each other, there may be a problem cutting
close to the end of the fragment.
Sequential
Enzyme sets that are not compatible ... chance
using restriction endonucleases or intron endonucleases, but the
Restriction Endonucleases 247
The NotI recognition site
5¢ GC
Ÿ
GGCCGC 3¢
3¢ . . . CGCCGG
Ÿ
CG 5¢
will not cleave whe...
Molecular Biology Problem Solver 30 docx
... . . . . . . 311
291
Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein
Copyright © 2001 by Wiley-Liss, Inc.
ISBNs: 0 -471 -37972-7 (Paper); 0 -471 -22390-5 (Electronic)
• ... appropriate
PCR strategy, to avoid problems, and to help you think your way
out of problems that do arise. While your research topic may be
unique, the solutions to most PCR problems...
Molecular Biology Problem Solver 38 docx
... . . . . . . . . 387
Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein
Copyright © 2001 by Wiley-Liss, Inc.
ISBNs: 0 -471 -37972-7 (Paper); 0 -471 -22390-5 (Electronic)
idea ... markers will provide precise molecular weight values.
The pre-stained recombinant proteins of known, reproducible
molecular weights discussed above can also determine the
mole...