Molecular Biology Problem Solver 45 pps
Molecular Biology Problem Solver 45 pps
... under-represented when working with screens and flours. When working with radioactive labels, this problem can be cor- rected by a combination of exposure at -70°C and sensitizing the film with a ... Autoradiography Film Static electricity can produce background signals on film. A solution to this problems has been proposed by Register (1999). The use of fluorescent crayolas to mark the orienta...
Ngày tải lên: 02/07/2014, 04:21
Molecular Biology Problem Solver 58 ppsx
... 447–448 quantitative capabilities of, 443– 445 resolution of, 441–442 screens for, 445 447 sensitivity of, 441–442 speed of, 441–442 Straight percent gels, 345 346 Stray light, as affecting spectrophotometer ... counters, 155 Screens, for storage phosphor imagers, 444, 445 447 SDS-PAGE, 337, 338, 349 electrical power for, 350–353 native PAGE versus, 345 348 problems with, 480–481 standardize...
Ngày tải lên: 02/07/2014, 04:21
Molecular Biology Problem Solver 10 pps
... Equipment 87 change due to temperature as they would in a manual calibration. A second source of problems is not matching a buffer or group of buffers with the correct buffer set in the meter. ... This is observed in ultrapure water and is part of the USP injection water testing protocol (USP 645, US Pharmacopeia). 90 Troutman et al.
Ngày tải lên: 02/07/2014, 04:21
Molecular Biology Problem Solver 11 pps
... causing the problem. Is the Sample the Problem? If the sample reading seems inappropriate, measure the pH of a buffer standard. A correct measurement of the standard points to a sample problem. ... recom- mended. Sometimes the “expected” value is not obtained for a mea- surement but the correct value is. The problem is simply an incorrect perceived value. Competing ions, sodium ions...
Ngày tải lên: 02/07/2014, 04:21
Molecular Biology Problem Solver 15 ppsx
... Radioactivity on the Outside of the Vial. Does This Indicate a Problem? . . . . . . . . . . . . 151 Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright ... particulate matter by centrifugation and a prefiltration step can help prevent clogging of the 0 .45 or 0.22 micron filters used to remove bacte- ria and fungi. These filters will not remove my...
Ngày tải lên: 02/07/2014, 04:21
Molecular Biology Problem Solver 16 ppsx
... fully below. A Wipe Test Detected Radioactivity on the Outside of the Vial. Does This Indicate a Problem? If you detect contamination on the outside of your vial, contact your RSO. She will tell
Ngày tải lên: 02/07/2014, 04:21
Molecular Biology Problem Solver 18 ppsx
... DNA and subsequent ethanol washes eliminate such a contaminant. Phenol can also be problematic. If you experience problems with DNA purified by a phenol-based strategy, apply chloroform to extract ... based on yields pub- lished for samples similar if not identical to your own. Do You Require High Molecular Weight Material? The average size of genomic DNA prepared will vary between commercia...
Ngày tải lên: 02/07/2014, 04:21
Molecular Biology Problem Solver 20 pps
... R., Kingston, R. E., Moore, D. D., Seidman, J. G., and Struhl, K. 1998. Current Protocols in Molecular Biology. Wiley, New York. Azad, A. K., Coote, J. G., and Parton, R. 1992. An improved method ... high. Limitations Cost, which may be minimized by reuse of resin. However cleaning of resin and its validation may be problematic. WHAT ARE THE OPTIONS FOR PURIFICATION AFTER IN VITRO REACTION...
Ngày tải lên: 02/07/2014, 04:21
Molecular Biology Problem Solver 22 ppsx
... total RNA isolation. In this section we address problems presented by difficult tissues and offer troubleshooting techniques to help over- come these problems. A separate section will discuss the ... break open the cells and simultaneously RNA Purification 203 9.5 – 7.5 – 4.4 – 2.4 – 1.35 – .24 – 12 3456 7891011 Figure 8.1 Assessing qual- ity of RNA preparation via agarose gel electrophoresis (...
Ngày tải lên: 02/07/2014, 04:21
Molecular Biology Problem Solver 31 ppsx
... electrophoresis may not allow you to reach the conclusion that there are two different mRNA forms if their molecular weights are similar.You might require a separation technique with greater resolving power. Suppose ... primer degradation by the 3¢–5¢ exonuclease activity (de Noronha and Mullins, 1992 and Skerra, 1992).The problem of reduced yield can be corrected by including an enzyme w...
Ngày tải lên: 02/07/2014, 04:21