Molecular Biology Problem Solver 44 ppt

... ac- celerants. Rate accelerators can increase the hybridization rate several-fold, but if background is problematic, reduce the concentration to 5%. The performance of dextran sulfate (and perhaps other ... in boxes is higher than in bags or bottles, but these larger volumes can help reduce background problems. Larger capacity also makes it feasible to simultaneously manipulate multiple filt...

Ngày tải lên: 02/07/2014, 04:21

... the wrong temperature. Solving Problems by Yourself In a perfect world, six steps will solve any problem: 1. Define the problem. What do you see? The first step in any problem- solving activity is ... nature of the problem without drawing conclusions. Understanding a problem consists of describing all the factual aspects about the problem. Do not try to determine what caused the probl...

Ngày tải lên: 02/07/2014, 04:21

... . . . . . . . . 44 What Is the Initial pH of the Water?. . . . . . . . . . . . . . . . . . . . 44 What Organics Can Be Present in the Water? . . . . . . . . . . . 45 What Other Problems Occur ... might be affected. Chemical Compatibility It is important to anticipate (or be able to diagnose) problems due to interaction of your buffer components with other solution components. Certain inor...

Ngày tải lên: 02/07/2014, 04:21

... apply little mechanical stress, so shearing is generally not a problem. Limitations If phenolic contaminants (i.e., from plants) are a problem, adding 1% polyvinylpyrrolidine to your extraction buffer ... http://web.bham.ac.uk/bcm4ght6/res.html, as does the E. coli Genetic Stock Center at Yale Univeristy (http://cgsc .biology. yale.edu). DNA Purification 181 based on solubility difference...

Ngày tải lên: 02/07/2014, 04:21

... desirable. Inhibition may become a problem if the DNA has contaminants that influence enzyme activity. Salt and other contaminants in the DNA solution are more likely to be problematic if the DNA solution

Ngày tải lên: 02/07/2014, 04:21

... water is also recommended, especially if one is proceeding to another step where EDTA might be a problem. The Volume of Enzyme in the Vial Appears Very Low. Did Leakage Occur during Shipment? Some ... nucleases. TROUBLESHOOTING What Can Cause a Simple Restriction Digestion to Fail? Faulty Enzyme or Problem Template Preparation? If the suspect enzyme fails to digest a second or control ta...

Ngày tải lên: 02/07/2014, 04:21

... from from 25 to 37 mg per A 260 unit. This approach is inexact, but it is reliable for common molecular biology techniques as long as its limitations are considered. Computer software that predicts ... 94.17 Experiment 3 dATP 99.40 92.02 dCTP 99.66 93.84 dGTP 99.39 92.68 dTTP 99.15 93.69 Experiment 4 dATP 99 .44 92.77 dCTP 99.59 93.89 dGTP 99.43 92.88 dTTP 99.19 93.65 Source: Data from A...

Ngày tải lên: 02/07/2014, 04:21

... Department of Microbiology, University of Cape Town. In Molecular Biology Techniques Manual: Third Molecular Biology Techniques Manual, V. E. Coyne et al., eds. http://www.uct.ac.za/microbiology/pcroptim.htm Standard ... regarding the molecular weight vs. relative mobility curve, and to Lee Olech and Dave Garfin for fruitful discussions about many of the questions in this chapter. M...

Ngày tải lên: 02/07/2014, 04:21

... methylene blue staining upon subsequent hybridization. Some newer dyes (SYBR ® Gold and SYBR ® Green, Molecular Probes Inc.) are promoted as noninterfering stains. Otherwise, in light of the inconsistencies ... times. Linearity of transfer needs to be tested empirically with dilution series of nucleic acid molecular weight markers. What Are the Indicators of a Functional Membrane? Membran...

Ngày tải lên: 02/07/2014, 04:21