Molecular Biology Problem Solver 43 pptx

... methylene blue staining upon subsequent hybridization. Some newer dyes (SYBR ® Gold and SYBR ® Green, Molecular Probes Inc.) are promoted as noninterfering stains. Otherwise, in light of the inconsistencies ... times. Linearity of transfer needs to be tested empirically with dilution series of nucleic acid molecular weight markers. What Are the Indicators of a Functional Membrane? Membran...

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... the wrong temperature. Solving Problems by Yourself In a perfect world, six steps will solve any problem: 1. Define the problem. What do you see? The first step in any problem- solving activity is ... nature of the problem without drawing conclusions. Understanding a problem consists of describing all the factual aspects about the problem. Do not try to determine what caused the probl...

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... apply little mechanical stress, so shearing is generally not a problem. Limitations If phenolic contaminants (i.e., from plants) are a problem, adding 1% polyvinylpyrrolidine to your extraction buffer ... http://web.bham.ac.uk/bcm4ght6/res.html, as does the E. coli Genetic Stock Center at Yale Univeristy (http://cgsc .biology. yale.edu). DNA Purification 181 based on solubility difference...

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... desirable. Inhibition may become a problem if the DNA has contaminants that influence enzyme activity. Salt and other contaminants in the DNA solution are more likely to be problematic if the DNA solution

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... water is also recommended, especially if one is proceeding to another step where EDTA might be a problem. The Volume of Enzyme in the Vial Appears Very Low. Did Leakage Occur during Shipment? Some ... nucleases. TROUBLESHOOTING What Can Cause a Simple Restriction Digestion to Fail? Faulty Enzyme or Problem Template Preparation? If the suspect enzyme fails to digest a second or control ta...

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... Department of Microbiology, University of Cape Town. In Molecular Biology Techniques Manual: Third Molecular Biology Techniques Manual, V. E. Coyne et al., eds. http://www.uct.ac.za/microbiology/pcroptim.htm Standard ... regarding the molecular weight vs. relative mobility curve, and to Lee Olech and Dave Garfin for fruitful discussions about many of the questions in this chapter. M...

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... 8–9 Reprobing in hybridization, 432 433 , 433 434 in Western blotting, 379, 380, 388–389 Reproducibility of DNA purification methods, 169 in electrophoresis, 341– 343 improving in electrophoresis, ... conditions, as source of problems, 21–22, 40–42 Storage phosphor imagers dynamic range of, 442– 443 erasure of, 447 in nucleic acid hybridization, 441–448 operation of, 441 problems with, 44...

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... MOLECULAR BIOLOGY PROBLEM SOLVER Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © ... will provide insight and perspective to help you solve problems that go beyond the content of this book. ix Preface MOLECULAR BIOLOGY PROBLEM SOLVER A LABORATORY GUIDE Edited by Alan S. Gerstein A ... address to receive your inquiries and co...

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... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 2001 by Wiley-Liss, ... the desired outcome. If the unexpected occurs, consider categorizing problems as either technical or global. Tech- nical problems are usually procedural in nature.The data obtained are eith...

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