Molecular Biology Problem Solver 42 ppsx
Molecular Biology Problem Solver 58 ppsx
... conditions, as source of
problems, 21–22, 40–42
Storage phosphor imagers
dynamic range of, 442–443
erasure of, 447
in nucleic acid hybridization,
441–448
operation of, 441
problems with, 447–448
quantitative ... 350–353
native PAGE versus, 345–348
problems with, 480–481
standardized gels for, 363
Sealed containment rooms, in
biosafety, 117
Secondary antibodies, 384
problems with, 395
Secondar...
Molecular Biology Problem Solver 1 pdf
... MOLECULAR BIOLOGY
PROBLEM SOLVER
Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein
Copyright © 20 01 by Wiley-Liss, Inc.
ISBNs: 0-4 71- 37972-7 (Paper); 0-4 71- 22390-5 ... Permissions Department, John Wiley & Sons, Inc., 605 Third Avenue,
New York, NY 10 158-0 012 , ( 212 ) 850-6 011 , fax ( 212 ) 850-6008, E-Mail:
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This p...
Molecular Biology Problem Solver 2 potx
... . . . . . . . . 9
Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein
Copyright © 20 01 by Wiley-Liss, Inc.
ISBNs: 0-471-379 72- 7 (Paper); 0-471 -22 390-5 (Electronic)
nique ... Project Will Define
You 2
Which Research Style Best Fits Your Situation? . . . . . . . . . . 2
Do You Have the Essential Resources? . . . . . . . . . . . . . . . . . . 2
Expe...
Molecular Biology Problem Solver 3 pdf
... . . . . . . . 19
Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein
Copyright © 2001 by Wiley-Liss, Inc.
ISBNs: 0-471 -37 972-7 (Paper); 0-471-2 239 0-5 (Electronic)
Big ... . . . . . . . . . . . . . 13
Does a Company Test Every Application for a
Product? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
How Well Will...
Molecular Biology Problem Solver 4 pptx
... the wrong temperature.
Solving Problems by Yourself
In a perfect world, six steps will solve any problem:
1. Define the problem. What do you see?
The first step in any problem- solving activity is ... nature of the problem without drawing conclusions.
Understanding a problem consists of describing all the factual
aspects about the problem. Do not try to determine what caused
the probl...
Molecular Biology Problem Solver 15 ppsx
... . . . . . . . . . . 150
A Wipe Test Detected Radioactivity on the Outside
of the Vial. Does This Indicate a Problem? . . . . . . . . . . . . 151
Molecular Biology Problem Solver: A Laboratory ... Requirements of Autoclaving?
The minimum requirements for sterilization are 15 lb of pres-
sure per square inch at 115 C for 15 minutes of sterilization time
(not including the exhaus...
Molecular Biology Problem Solver 16 ppsx
... . . . . . . . 164
How Can You Concentrate a Radioactive Solution? . . . . . . 164
Bibliography . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 166
Appendix ... . . . . . . . . . . . . 161
What Can You Do to Achieve Minimum Radioactive
Dose? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 162
How Can You Organ...
Molecular Biology Problem Solver 18 ppsx
... . . . . . . 185
Silica Resin-Based Strategies . . . . . . . . . . . . . . . . . . . . . . . . . . . 186
Isolation from Electrophoresis Gels . . . . . . . . . . . . . . . . . . . . 187
What Are ... DNA and subsequent ethanol washes eliminate such a
contaminant. Phenol can also be problematic. If you experience
problems with DNA purified by a phenol-based strategy, apply
chloroform to extr...
Molecular Biology Problem Solver 22 ppsx
... total
RNA isolation. In this section we address problems presented by
difficult tissues and offer troubleshooting techniques to help over-
come these problems. A separate section will discuss the ... RNA-
binding filters. Increasing the ratio of sample mass: volume of lysis
buffer can help alleviate this problem in filter-based isolations.
Multiple acid–phenol extractions can be done to ensure...
Molecular Biology Problem Solver 31 ppsx
... electrophoresis may not allow you to reach the conclusion that
there are two different mRNA forms if their molecular weights
are similar.You might require a separation technique with greater
resolving power.
Suppose ... primer
degradation by the 3¢–5¢ exonuclease activity (de Noronha and
Mullins, 1992 and Skerra, 1992).The problem of reduced yield can
be corrected by including an enzyme w...
Molecular Biology Problem Solver 50 ppsx
... 521
Planning the Baculovirus Experiment . . . . . . . . . . . . . . . . . . . . . 521
Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein
Copyright © 2001 by Wiley-Liss, ... an Appropriate Expression Host . . . . . . . . . . . . . . . 501
Selecting an Appropriate Expression Vector . . . . . . . . . . . . . 506
Implementing the Eukaryotic Expression Expe...
Molecular Biology Problem Solver 54 ppsx
... Acad.
Sci. U.S.A. 95:2509–2514.
Higgins, D. R., and Cregg, J. M. 1998. Pichia Protocols: Methods in Molecular
Biology, vol 103. Humana Press, Totowa NJ.
Hill-Perkins, M. S., and Possee, R. D. 1990. A baculovirus ... baculovirus-infected insect cells:
Expression, purification and characterization. Prot. Expr. Purif. 10: 154 161.
Gignac, S. M., Brauer, S., Hane, B., Quentmeier, H., and Drexle...
Molecular Biology Problem Solver 57 ppsx
... hybridization
experiments, 402
Problem reduction, 2
Problem resolution, failed, 28
Problems. See also Global problems;
Technical problems
with acrylamide polymerization,
342–343
Index 557
Ink, for marking ... (piperazine diacrylamide), as
crosslinker, 338
Peer-reviewed journals, 5
Pelleting, 56, 57, 65
improving, 66–67
Pellets, problems with RNA, 220–221
Peptide electrophoresis, buffer...
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