Molecular Biology Problem Solver 41 pot

... . . . . . . . . . . . . . . . . . . . . . 412 Is It Necessary to Purify Every Probe? . . . . . . . . . . . . . . . . . 413 Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan ... . . . . . . . . . 441 Detection by Storage Phosphor Imagers . . . . . . . . . . . . . . . . . . 441 How Do Phosphor Imagers Work? . . . . . . . . . . . . . . . . . . . . . 441 Is a Stora...

Ngày tải lên: 02/07/2014, 04:21

... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 2001 by Wiley-Liss, ... the desired outcome. If the unexpected occurs, consider categorizing problems as either technical or global. Tech- nical problems are usually procedural in nature.The data obtained are eith...

Ngày tải lên: 02/07/2014, 04:21

... manufacturer will indicate those speeds that could cause centrifugation media to precipitate and potentially damage the rotor and or centrifuge (Figure 4.4). Is a Vertical Rotor the Right Angle ... the DNA as the gra- dient re-orientes. A near-vertical rotor from Beckman-Coulter eliminates this problem (Figure 4.6).The 9° angle of this rotor allows the RNA to pellet to the bottom of the tub...

Ngày tải lên: 02/07/2014, 04:21

... peptide bonds form a purple complex with copper sulphate in the pres- ence of sodium potassium tartrate and potassium iodide in the reagent. There are very few interfering agents apart from ammonium ... Moore, D. D., Seidman, J. G., Smith, J. A., and Struhl, K. (eds.), 1998. Current Protocols in Molecular Biology. Wiley, New York. Beckman-Coulter Corporation. Application Note A—1790A. 199...

Ngày tải lên: 02/07/2014, 04:21

... that produces sparks or smoke has the potential to cause harm by electric shock or fire. If in doubt, leave the room and call the fire department. What Are the Potential Sources of Contamination ... appropriate types of laboratory clothing that should be worn in the microbiology laboratory, important safety equipment and supplies, and potential sources of harm. 122 Haidaris and Brownlow ... p...

Ngày tải lên: 02/07/2014, 04:21

... avoided because it may increase primer-dimer formation and reduce PCR effi- ciency. This is more problematic when you have a low number of target gene copies. • Avoid runs of G/C, especially guanidine. • When ... have the correct sequence. Inefficient desalting, incorrect labeling, and other quality control problems can ruin a primer’s performance. 314 Aoyagi which increases specificity of the...

Ngày tải lên: 02/07/2014, 04:21

... not controlled, the pore size will vary from day to day. Symptoms of Problems with Catalyst Potency The best indicator of a problem catalyst is poor polymeriza- tion of the gel. If you’re confident ... principle here is that impurities in the water cause problems, and the purest water avail- able should be used for electrophoresis to help prevent these problems. Bacteria in your water purifi...

Ngày tải lên: 02/07/2014, 04:21

... 1997; Molloy, 2000).Very low and very high molecular weight proteins may also be problematic, as well as basic proteins. Procedures to avoid these problems must be worked out for each sample. How ... every gel. Is the Problem Caused by the Sample or the Sample Buffer? For lyophilized standards, make fresh standard buffer. Some- times it is difficult to determine whether the problem is i...

Ngày tải lên: 02/07/2014, 04:21

... can be used to report only approximate molecular weights (within 10,000 daltons of the molecular weight as determined by an unstained standard). The molecular weight values of most pre-stained ... to Determine the Molecular Weight of an Unknown Protein? Pre-stained protein standards usually run as broad, fuzzy bands, making them useful for approximate, but not exact, molecular we...

Ngày tải lên: 02/07/2014, 04:21

... loading buffer, DNaseI (10mg/ml), extended heating of the sample, or sonication should alleviate the problem. After electrophoresis, the gel should be stained (e.g., Coomassie Blue) to visualize the ... whole cell lysate. If expres- sion is good, an induced band will clearly be seen at the predicted molecular weight, and this will be absent in the no-insert control culture. If no band is...

Ngày tải lên: 02/07/2014, 04:21